SONGÜL ÇAKMAKÇI, Emel ÖZ, Kübra ÇAKIROĞLU, Atilla POLAT, İlhami GÜLÇİN, Şaziye ILGAZ, Kimya SEYYEDCHERAGHI, İzzet ÖZHAMAMCI

Probiotic Shelf Life, Antioxidant, Sensory, Physical and Chemical Properties of Yogurts Produced with Lactobacillus acidophilus and Green Tea Powder

The aim of the present study was to determine the effects of the addition of green tea powder (GTP) in the production of yogurt on survival of Lactobacillus acidophilus (P) and the yogurt properties. Four yogurt groups (yogurt without P and GTP, Control, C; yogurt with P and without GTP, PC; P + 1% GTP; and P + 2% GTP) were produced. The yogurt samples were stored at 4°C. The addition of GTP into milk did not affect the viability of yogurt bacteria during fermentation. The highest count of L. acidophilus was detected in P + 2% GTP. The L. acidophilus count was high up to the 7th day (7.54 log cfu/g). Yeast and mold were not counted (

Lactobacillus acidophilus ve Yeşil Çay Pudrası İle Üretilen Yoğurtların Probiyotik Raf Ömrü, Antioksidan, Duyusal, Fiziksel ve Kimyasal Özellikleri

Bu araştırmanın amacı, yoğurt üretiminde yeşil çay pudrası (GTP) ilavesinin Lactobacillus acidophilus (P) canlılığı ve yoğurt özellikleri üzerine etkilerini belirlemekti. Bu amaçla, dört farklı yoğurt çeşidi üretildi [P ve GTP ilavesiz yoğurt (Kontrol, C); sadece P içeren yogurt (PC); P + %1 GTP ilaveli yoğurt ve P + %2 GTP ilaveli yoğurt]. Yoğurt örnekleri 4°C’de muhafaza edildi. Süte GTP ilavesi, fermantasyon sırasında yoğurt bakterilerinin canlılığını etkilemedi. En yüksek L. acidophilus sayısı P + %2 GTP ilaveli yoğurtta tespit edildi. L. acidophilus sayısı 7. güne kadar yüksekti (7.54 log kob/g). Depolama süresince yoğurt örneklerinin hiçbirinde maya ve küf bulunmadı (

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1. Tamime AY, Robinson RK: Yoghurt: Science and Technology. Cambridge; Woodhead Publishing Limited, 2007.

2. Najgebauer-Lejko D: Effect of green tea supplementation on the microbiological, antioxidant, and sensory properties of probiotic milks. Dairy Sci Technol, 94, 327-339, 2014. DOI: 10.1007/s13594-014-0165-6

3. Mishra V, Shah C, Mokashe N, Chavan R, Yadav H, Prajapati J: Probiotics as potential antioxidants: A systematic review. J Agric Food Chem, 63, 3615-3626, 2015. DOI: 10.1021/jf506326t

4. Kailasapathy K, Harmstorf I, Phillips M: Survival of Lactobacillus acidophilus and Bifidobacterium animalis ssp. lactis in stirred fruit yogurts. LWT-Food Sci Technol, 41, 1317-1322, 2008. DOI: 10.1016/j.lwt.2007.08.009

5. Tripathi MK, Giri SK: Probiotic functional foods: Survival of probiotics during processing and storage. J Funct Foods, 9, 25-241, 2014. DOI: 10.1016/j.jff.2014.04.030

6. Namal Senanayake SPJ: Green tea extract: Chemistry, antioxidant properties and food applications - A review. J Funct Foods, 5, 1529-1541, 2013. DOI: 10.1016/j.jff.2013.08.011

7. Najgebauer-Lejko D, Sady M, Grega T, Walczycka M: The impact of tea supplementation on microflora, pH and antioxidant capacity of yoghurt. Int Dairy J, 21, 568-574, 2011. DOI: 10.1016/j.idairyj.2011.03.003

8. Marhamatizadeh MH, Ehsandoost E, Gholami P: The influence of green tea (Camellia sinensis L.) extract on characteristic of probiotic bacteria in milk and yoghurt during fermentation and refrigerated storage. Int J Farm Alli Sci, 2, 599-606, 2013.

9. Zhao D, Shah NP: Tea and soybean extracts in combination with milk fermentation inhibit growth and enterocyte adherence of selected foodborne pathogens. Food Chem, 180, 306-316, 2015. DOI: 10.1016/j. foodchem.2015.02.016

10. Ankolekar C, Johnson D, Pinto MS, Johnson K, Labbe R, Shetty, K: Inhibitory potential of tea polyphenolics and influence of extraction time against Helicobacter pylori and lack of inhibition of beneficial lactic acid bacteria. J Med Food, 14, 1321-1329, 2011. DOI: 10.1089/jmf.2010.0237

11. Amirdivani S, Baba ASHJ: Green tea yogurt: major phenolic compounds and microbial growth. J Food Sci Technol, 52, 4652-4660, 2015. DOI: 10.1007/s13197-014-1670-6

12. Lim ES: Effect of green tea supplementation on probiotic potential, physico-chemical, and functional properties of yogurt. Korean J Microbiol, 53, 103-117, 2017. DOI: 10.7845/kjm.2017.7035

13. Harrigan WF: Laboratory Methods in Food Microbiology, 3rd ed., Academic Press, San Diego, CA, 1998.

14. Vinderola CG, Reinheimer JA: Culture media for the enumeration of Bifidobacterium bifidum and Lactobacillus acidophilus in the presence of yoghurt bacteria. Int Dairy J, 9, 497-505, 1999. DOI: 10.1016/S0958- 6946(99)00120-X

15. AOAC: Official Methods of Analysis, 15th ed., Association of Official Analytical Chemists, Washington, DC, 1990.

16. Atamer M, Sezgin E: Yoğurtlarda kuru madde artırımının pıhtının fiziksel özellikleri üzerine etkisi. Gıda, 11, 327-331, 1986.

17. Aksu K, Özgeriş B, Taslimi P, Naderi A, Gülçin İ, Göksu S: Antioxidant activity, acetylcholinesterase and carbonic anhydrase inhibitory properties of novel ureas derived from phenethylamines. Arch Pharm, 349, 944-954, 2016. DOI: 10.1002/ardp.201600183

18. Apak R, Güçlü K, Özyürek M, Karademir SE: A novel total antioxidant capacity index for dietary polyphenols, vitamin C and E, using their cupric ion reducing capability in the presence of neocuproine: The CUPRAC method. J Agric Food Chem, 52, 7970-7981, 2004. DOI: 10.1021/ jf048741x

19. Gülçin İ, Topal F, Öztürk Sarikaya SB, Bursal E, Bilsel G, Gören AC: Polyphenol contents and antioxidant properties of medlar (Mespilus germanica L.). Rec Nat Prod, 5, 158-175, 2011.

20. Blois MS: Antioxidant determinations by the use of a stable free radical. Nature, 26, 1199-1200, 1958.

21. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice- Evans C: Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med, 26, 1231-1237, 1999. DOI: 10.1016/s0891-5849(98)00315-3

22. Fogliano V, Verde V. Randazzo G, Ritieni A: Method for measuring antioxidant activity and its application to monitoring the antioxidant capacity of wines. J Agric Food Chem, 47, 1035-1040, 1999. DOI: 10.1021/ jf980496s

23. Gülçin İ, Topal F, Çakmakçı R, Gören AC, Bilsel M, Erdoğan U: Pomological features, nutritional quality, polyphenol content analysis and antioxidant properties of domesticated and three wild ecotype forms of raspberries (Rubus idaeus L.). J Food Sci, 76, C585-C593, 2011. DOI: 10.1111/j.1750-3841.2011.02142.x

24. Gülçin İ, Daştan A: Synthesis of dimeric phenol derivatives and determination of in vitro antioxidant and radical scavenging activities. J Enzyme Inhib Med Chem, 22, 685-695, 2007. DOI: 10.1080/ 14756360601164903

25. Singleton VL, Rossi JA: Colorimetry of total phenolics with phosphomolybdic phosphotungstic acid reagents. Am J Enol Vitic, 16, 144-148, 1965.

26. Ertem H, Cakmakci S: Shelf life and quality of probiotic yogurt produced with Lactobacillus acidophilus and Gobdin. Int J Food Sci Technol, 53, 776-783, 2018. DOI: 10.1111/ijfs.13653

27. Kullen MJ, Klaenhammer TR: Identification of the pH-inducible, proton-translocating F1F0-ATPase (atpBEFHAGDC) operon of Lactobacillus acidophilus by differential display: Gene structure, cloning and characterization. Mol Microbiol, 33, 1152-1161, 1999. DOI: 10.1046/j.1365- 2958.1999.01557.x

28. Cakmakci S, Tahmas-Kahyaoglu D, Erkaya T, Cebi K, Hayaloglu AA: β-carotene contents and quality properties of set type yoghurt supplemented with carrot juice and sugar. J Food Process Pres, 38, 1155- 1163, 2014. DOI: 10.1111/jfpp.12075

29. Lee HC, Jenner AM, Low CS, Lee YK: Effect of tea phenolics and their aromatic fecal bacterial metabolites on intestinal microbiota. Res Microbiol, 157, 876-884, 2006. DOI: 10.1016/j.resmic.2006.07.004

30. López de Lacey AM, Pérez-Santin E, López-Caballero ME, Montero P: Survival and metabolic activity of probiotic bacteria in green tea. LWT Food Sci Technol, 55, 314-322, 2014. DOI: 10.1016/j.lwt.2013.08.021

31. Turgut T, Cakmakci S: Probiotic strawberry yogurts: Microbiological, chemical and sensory properties. Probiotics Antimicrob Proteins, 10, 64-70, 2018. DOI: 10.1007/s12602-017-9278-6

32. Shah N, Jelen P: Survival of lactic acid bacteria and their lactases under acidic conditions. J Food Sci, 55, 506-509, 1990. DOI: 10.1111/ j.1365-2621.1990.tb06797.x

33. Turgut T: The effect of microwave heating on the some quality properties and shelf life of yoghurt. Kafkas Univ Vet Fak Derg, 22 (6): 809- 814, 2016. DOI: 10.9775/kvfd.2016.14875

34. Gülçin İ: Antioxidant activity of food constituents-An overview. Arch Toxicol, 86, 345-391, 2012. DOI: 10.1007/s00204-011-0774-2

35. Bursal E, Köksal E, Gülçin İ, Bilsel G, Gören AC: Antioxidant activity and polyphenol content of cherry stem (Cerasus avium L.) determined by LC-MS/MS. Food Res Int, 51, 66-74, 2013. DOI: 10.1016/j. foodres.2012.11.022

36. Chai PC, Long LH, Halliwell B: Contribution of hydrogen peroxide to the cytotoxicity of green tea and red wines. Biochem Biophys Res Commun, 304, 650-654, 2003. DOI: 10.1016/S0006-291X(03)00655-7

37. Ranjbar Nedamani E, Mahoonak AS, Ghorban, M, Kashaninejad M: Evaluation of antioxidant interactions in combined extracts of green tea (Camellia sinensis), rosemary (Rosmarinus officinalis) and oak fruit (Quercus branti). J Food Sci Technol, 52, 4565-4571, 2015. DOI: 10.1007/ s13197-014-1497-1

38. Drake MA. 2007: Invited review: Sensory analysis of dairy foods J Dairy Sci, 90, 4925-4937, 2007. DOI: 10.3168/jds.2007-0332

39. Sharif MK, Butt MS, Sharif HR, Nasir M: Sensory Evaluation and Consumer Acceptability. In, Handbook of Food Science and Technology, Chapter 14, 361-386, 2017.

40. Yang J, Lee J: Application of sensory descriptive analysis and consumer studies to investigate traditional and authentic foods: A review. Foods, 8 (2): 54, 2019. DOI: 10.3390/foods8020054

41. Tarakçı Z: Influence of kiwi marmalade on the rheology characteristics, color values and sensorial acceptability of fruit yogurt. Kafkas Univ Vet Fak Derg, 16 (2): 173-178, 2010. DOI: 10.9775/kvfd.2009.273

42. Wang H, Provan GJ, Helliwell K: Tea flavonoids: Their functions, utilisation and analysis. Trends Food Sci Technol, 11, 152-160, 2000. DOI: 10.1016/S0924-2244(00)00061-3

43. Chaturvedula VSP, Prakash I: The aroma, taste, color and bioactive constituents of tea. J Med Plants Res, 5, 2110-2124, 2011.