Qiu-Yong CHEN, Ru-Jing CHEN, Xue-Min WU, Yong-Liang CHE, Bo HOU, Chen-Yan WANG, Long-Bai WANG, Lun-Jiang ZHOU

Study on the Antibody Level Differences of PED IgG and IgA, TGE IgG and PoR IgG After Immunization with Different Porcine Viral Diarrhea Vaccine Combinations

To prevent porcine viral diarrhea, a vaccine combination that can provide good antibody levels needs to be determined. In this study, we screened 30 pregnant sows divided into six experimental groups, namely, five immunized groups and one control group, to investigate the antibody level differences of different vaccine combinations on porcine epidemic diarrhea (PED), porcine transmissible gastroenteritis (TGE), porcine rotavirus (PoR) IgG, and PED IgA. The antibody level was detected by ELISA. Results showed that the antibody levels of PED and TGE IgG in serum and PED IgA in breast milk of the “PT+PT*” vaccine combination group were higher than those of the other groups, and vaccine combination including “PTR” could stimulate the sows to produce PoR IgG antibody. These findings revealed that the vaccine combination of “PT+PT*” is optimal for preventing porcine viral diarrhea, and “PTR+PT*” may be an alternative option in areas under PoRV infection risk. This study suggested that pig farms should select suitable immunization on the basis of the local epidemic situation of porcine viral diarrhea.

Farklı Domuz Viral Diyare Aşı Kombinasyonları İle İmmunizasyon Sonrası PED IgG ve IgA, TGE IgG ve PoR IgG Antikor Seviyelerindeki Farklılıkların Araştırılması

Domuz viral diyareyi önlemek için iyi antikor seviyesi sağlayan bir aşı kombinasyonunu belirlemeye ihtiyaç bulunmaktadır. Bu çalışmada, farklı aşı kombinasyonlarının domuz epidemik diyare, domuz bulaşıcı gastroenteritisi ve domuz rotavirus IgG seviyeleri ile domuz epidemik diyare IgA seviyesine etkilerini araştırmak amacıyla 30 gebe domuz 5’i immunize grup 1’i kontrol olmak üzere altı gruba ayrıldı. Antikor seviyeleri ELISA ile belirlendi. Elde edilen sonuçlar, domuz epidemik diyare ve domuz bulaşıcı gastroenteritisi serum IgG seviyeleri ile meme sütünde domuz epidemik diyare IgA seviyesinin “PT+PT*” aşı kombinasyonu grubunda diğerlerinden daha yüksek olduğunu ve “PTR”yi içeren aşı kombinasyonunun domuzları domuz rotavirus IgG antikoru üretmek üzere stimüle ettiğini gösterdi. Bu sonuçlar “PT+PT*” aşı kombinasyonunun domuz viral diyareyi önlemede en iyi olduğunu ve “PTR+PT*”in domuz rotavirus enfeksiyon riski bulunan bölgelerde bir alternatif olabileceğini gösterdi. Çalışma sonucunda domuz viral diyarenin bölgesel epidemik durumuna göre domuz çiftliklerinin immunizasyon seçiminde bulunması önerilir.

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