Umut Çağın ARI, Necdet Cankat LEHİMCİOĞLU, Yavuz ÖZTÜRKLER, Savaş YILDIZ

Progesteron Temelli Östrus Senkronizasyonu Protokolüne Tabi Tutulan Repeat Breeder İneklerde Suni Tohumlama Sonrası İntrauterin Carvacrol İnfüzyonunun Gebelik Oranları Üzerine Etkisi

Repeat Breeder (RB), süt ineklerinde ekonomik kaybın önemli nedenlerinden biridir. Bu çalışmanın amacı, RB ineklerinde zamanlanmış suni tohumlama sonrası intrauterin carvacrol uygulamasının gebelik oranlarına (GO) etkilerini belirlemektir. Çalışma, 3 defadan fazla geri dönen, genital sisteminde önemli patolojik bozukluk saptanmayan 155 adet RB inekte yürütüldü. Tüm inekler aşağıdaki östrus senkronizasyonu yöntemine tabi tutuldu: 1.55 g progesteron içeren bir intra-vajinal aparat (IVA), 9 gün boyunca yerleştirildi ve IVA çıkarılmasından bir gün önce bir doz PGF2α uygulandı ve suni tohumlamadan 6 saat önce bir doz GnRH enjekte edildi. Tüm inekler, IVA çıkarıldıktan 56 saat sonra tohumlandı. Tüm hayvanlar rastgele üç gruba ayrıldı. Uygulama grupları olan grup I (GI; n=52) ve grup II (GII; n=51)’ye sırasıyla %0.1 seyreltilmiş Carvacrol ve %0.1 Lugol (GII) çözeltileri verildi. Kontrol grubu grup III’e (GIII; n=51) sadece normal serum fizyolojik infüze edildi. Tüm infüzyonlar, tohumlamadan yarım saat sonra uterus içine bir doz 30-50 mL (uterus boyutuna göre) olacak şekilde uygulandı. Tohumlamadan 21 ila 30 gün sonra yapılan gözlemlerde, geri dönmeyenlerin oranı (NRR), GI, GII ve GIII gruplarında sırasıyla %71.15, %69.23 ve %68.62 olarak belirlendi. Suni tohumlamayı takiben 60. günde yapılan rektal palpasyonla teşhis edilen GO ise GI, GII ve GIII’de sırasıyla %67.30, %63.46 ve %46.15 olarak saptandı. Ancak, gruplar arasındaki farklar istatistiksel olarak anlamlı değildi. Sonuç olarak: RB ineklerin tedavisinde, uterusa carvacrol infüzyonunun gebelik oranlarını bir dereceye kadar arttırdığı için tercih edilebileceği kanaatine varıldı. Fakat, bu sonuçları pekiştirmek için başka araştırmalara ihtiyaç olduğu düşünülmektedir.

The Effect of Intrauterine Infusion of Carvacrol After Insemination on Conception Rate in Repeat Breeder Cows Subjected to Progesteron Based Estrus Synchronization Protocol

Repeat breeder (RB) is one of the crucial causes of economic loss in dairy cows. The aim of this study was to determine the effects ofintrauterine carvacrol administration after timed artificial insemination (TAI) on the conception rate (CR) in RB cows. The study involved 155RB cows returned to service for more than three times and without any significant pathologic defects in genital tract. All cows were subjectedto following estrus synchronization method: An intra-vaginal apparatus (IVA) containing 1.55 g progesteron were inserted for 9 days andadministered one dose of PGF2α one day before removing IVA, then injected one dose of GnRH 6 h before TAI. All the cows were inseminatedin 56th h after removing IVA. All RB cows were randomly divided to three groups. Group I (GI; n=52) and Group II (GII; n=51), treatmentsgroups were infused with 0.1% diluted Carvacrol and 0.1% Lugol’s (GII) solution. Group III (GIII; n=51) the control group, was received normalsaline only. All infusions were administered one dose of 30-50 mL (according to uterine size) into uterus half hour after insemination. In theobservations performed in 21 to 30 days after insemination, non-return rates (NRR) were 71.15%, 69.23% and 68.62% in groups of GI, GII andGIII, respectively. CR detected by rectal palpation at 60 days following artificial insemination were 67.30%, 63.46% and 46.15% in GI, GII andGIII, respectively. However, the differences between groups were not statistically significant. As a result: In the treatment of RB cows, it maybe suggested that carvacrol infusion to the uterus may be preferred as it has improved pregnancy rates to some extent. Nevertheless, furtherinvestigations are needed to confirm these results.

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1. Pothmann H, Prunner I, Wagener K, Jaureguiberry M, de la Sota RL, Erber R, Aurich C, Ehling-Schulz M, Drillich M: The prevalence of subclinical endometritis and intrauterine infections in repeat breeder cows. Theriogenology, 83, 1249-1253, 2015. DOI: 10.1016/j. theriogenology.2015.01.013
2. Öner Y, Yılmaz O, Okut H, Ata N, Yılmazbaş-Mecitoğlu G, Keskin A: Associations between GH, PRL, STAT5A, OPN, PIT-1, LEP and FGF2 polymorphisms and fertility in Holstein-Friesian heifers. Kafkas Univ Vet Fak Derg, 23 (4): 527-534. 2017. DOI: 10.9775/kvfd.2016.17192
3. Bartletta PC, Kirka JH, Mather EC: Repeated insemination in Michigan Holstein-Friesian cattle: Incidence, descriptive epidemiology and estimated economic impact. Theriogenology, 26 (3): 309-322, 1986. DOI: 10.1016/0093-691x(86)90150-0
4. Thakur S, Singh M, Vasishta NK: Study on etiology of repeat breeding in Himchal Pradesh. Punjab Vet J, 4, 27-29, 2006.
5. Gupta AG, Deopurkar RL: Microbial study of gynaecologyical infection in cattle. IJAR, 14, 118-119, 2005.
6. Narayan Rao V: Infertility problems in crossbred cows in Andhra Pradesh. ISSAR Second Annual convention and National symposium held at Universityof Agricultural Sciences, Bangalore, and pp.191-195, 1980.
7. Bhosrekar M: Investigation into the incidence and causes of repeat breeding in dairy cattle at National Dairy Research Institute, Karnal (Haryana). India Vet J, 50, 418-429, 1973
8. Singh M, Sharma A, Sharma A and Kumar P: Repeat breeding and its treatment in dairy cattle of Himachal Pradesh (India) - A Revıew. Indian J Anim Reprod, 38 (2): 1-5, 2017.
9. Hartigan PJ, Murphy JA, Nunn WR, Griffin JFT: An investigation into the causes of reproductive failure in dairy cows: Intra uterine infection and endometrial histopathology in clinically normal-repeat breeder cows. Irish Vet J, 2, 245-247, 1972.
10. Levine HD: The repeat breeder cow. Bov Pract, 33, 97-105, 1999.
11. Rani P, Dutt R, Singh G and Chandolia RK: Embryonic mortality in cattle- A review. Int J Curr Microbiol App Sci, 7 (7): 1501-1516, 2018. DOI: 10.20546/ijcmas.2018.707.177
12. Singh NJ, Singh A and Patel AK: Employing the effect of gentamycin and enrofloxacin treatment on pregnancy rate of repeat breeder dairy cross bred cows. World J Pharmaceut Res, 4 (8): 1144-1148, 2015.
13. Salasel B, Mokhtari A, Taktaz T: Prevalence, risk factors for and impact of subclinical endometritis in repeat breeder dairy cows. Theriogenology, 74 (7): 1271-1278, 2010. DOI: 10.1016/j.theriogenology.2010.05.033
14. Sarıbay MS, Köse AM, Yılmaz MA: Repeat breeder ineklerin tedavisinde GnRH ve gonadotropinlerin (LH, hCG, PMSG) kullanımı. Lalahan Hay Araşt Enst Derg, 58 (1): 34-41, 2018.
15. Oxender WD, Seguin BE: Bovine intrauterine therapy. J Am Vet Med Assoc, 168, 217-219, 1976.
16. Polat B, Cengiz M, Çolak A, Cannazik O: Comparison of ıntrauterine ozone and rifaximine treatment in cows with subclinical endometritis. Kafkas Univ Vet Fak Derg, 21 (5): 773-776, 2015. DOI: 10.9775/kvfd.2015.13690
17. Armengol R, Fraile L: Comparison of two treatment strategies for cows with metritis in high risk lactating dairy cows. Theriogenology, 83 (8): 1344–1351, 2015. DOI: 10.1016/j.theriogenology.2015.01.024
18. Öztürkler Y, Uçar Ö: İneklerde suni tohumlama başarısını artırıcı uygulamalar. Kafkas Univ Vet Fak Derg, 9 (2): 219-222, 2003.
19. Oral H, Kuru M, Kulaksız R, Kaya S: Kronik endometritisli ineklerde intrauterin uygulanan kekik yağının gebe kalma oranı üzerine etkisi. Lalahan Hay Araşt Enst Derg, 54 (2): 57-61, 2014.
20. Khillare K, Birade HS, Maini S, Ravikanth K: Role of polyherbal intrauterine infusion in treatment ofvarious reproductive disorders in cattle. Vet World, 3 (8): 373-374, 2010.
21. Friedman M: Chemistry and multibeneficial bioactivities of carvacrol (4-isoprpyl-2-methylphenol), a component of essential oils produced by aromatic plants and spicies. J Agric Food Chem, 62 (31): 7652-7670, 2014. DOI: 10.1021/jf5023862
22. Nostro A, Roccaro AS, Bisignano G, Marino A, Cannatelli MA, Pizzimenti FC, Cioni PL, Procopio F, Blanco AR: Effect of oregano, carvacrol and thymol on Staphylococcus epidermidis biofilms. J Med Microbiol, 56, 519-523, 2007.
23. Bnyan IA, Abid AT, Hamid HN: Antibacterial activity of carvacrol against different types of bacteria. J Nat Sci Res, 4 (9): 13-16, 2014.
24. Baser KHC: Biological and pharmacological activites of carvacrol and carvacrol bearingessansial oils. Curr Pharm Desing, 14 (29): 3106-3119, 2008. DOI: 10.2174/138161208786404227
25. Austgulen LT, Solheim E, Scheline RR: Metabolism in rats of p-cymene derivatives: Carvacrol and thymol. Pharmacol Toxicol, 61 (2): 98-102, 1987. DOI: 10.1111/j.1600-0773.1987.tb01783.x
26. O’Donnell SL: The efficacy of antibiotic residue screening tests for the detection of natural antimicrobials in milk. MSc Thesis, University of Connecticut, United States of America, 175, 2011.
27. Baskaran SA, Kazmer GW, Hinckley L, Andrew SM, Venkitanarayanan K: Antibacterial effect of plant-derived antimicrobialson major bacterial mastitis pathogens in vitro. J Dairy Sci, 92, 1423-1429, 2008. DOI: 10.3168/ jds.2008-1384
28. Alagawany M, Abd El-Hack ME, Farag MR, Tiwari R, Dhama K: Biological effects and modes of action of carvacrol in animal and poultry production and health - A review. Adv Anim Vet Sci, 3 (2s): 73-84, 2015. DOI: 10.14737/journal.aavs/2015/3.2s.73.84
29. Suntres ZE, Coccimiglo J, Alipour M: The bioactivity and toxicological actions of carvacrol. Crit Rev Food Sci Nutr, 55 (3): 304-318, 2015. DOI: 10.1080/10408398.2011.653458
30. Pinedo PJ, Velez JS, Bothe H, Merchan D, Piñeiro JM, Risco CA: Effect of intrauterine infusion of an organic-certified product on uterine health, survival, and fertility of dairy cows with toxic puerperal metritis. J Dairy Sci, 98, 3120-3132, 2015. DOI: 10.3168/jds.2014-8944
31. Öztürkler Y, Uçar Ö, Lehimcioğlu NC: İneklerde suni tohumlamayı takiben intra uterin ilaç uygulamasının gebelik oranları üzerine etkisi. Kafkas Univ Vet Fak Derg, 7 (2): 197-200, 2001.
32. İleri İK: Suni Tohumlamaya bağlı olarak gebelik oranlarını artırıcı klinik tedavi uygulamaları. In, İleri İK, Ak K, Papuççoğlu S. Usta S (Eds): Reprodüksiyon ve Suni Tohumlama İstanbul Üniversitesi Veteriner Fakültesi Yayını, Ders Notu No:23, İstanbul 141-145, 1994.
33. Çolak A, Öztürkler Y: Repeat breeder ineklerde rifaksimin ve lügol solüsyonu uygulamasını takiben, östrus sinkronizasyonu ve suni tohumlamanın gebelik oranı üzerine etkisi. VETAŞ Bülten, 3, 8-10, 1998.
34. Öztürkler Y, Uçar Ö, Yıldız S, Güngör Ö: The effect of hCG and gentamicin administration related to artificial insemination following oestrus synchronisation upon the calvingrates in repeat breeder cows. Kafkas Univ Vet Fak Derg, 7 (2): 207-211, 2001.
35. Tasal İ: İneklerde repeat breeder (Dönen İnek) sendromunun klinik yönden irdelenmesi. Turkiye Klinikleri J Vet Sci, 2 (1): 74-84, 2011
36. Vandeplassche M: Neu vergleichende der involution und der puerperalen metritis bei stute, kuh und sau. Mh Vet Med, 36, 804-807, 1981.
37. Ahmed WM, El-khadrawy HH, Hanafi EM, Amal HA, Shalaby SA: Clinical perspective of repeat breeding syndrome in buffaloes. J Am Sci, 6 (11): 661-666, 2010.
38. Sharma S, Sing M: Mycotic endometritisin cows and its therapeutic managment. Intas Polivet, 13 (1): 29-30, 2012.
39. Kasimanickam R, Duffield TF, Foster RA, Gartley CJ, Leslie KE, Walton JS, Johnson WH: A comparison of the cytobrush and uterine lavage techniques toevaluate endometrial cytology in clinically normal postpartum dairy cows. Can Vet J, 46 (3): 255-259, 2005.
40. Sheldon IM, Noakes DE, Rycroft AN, Pfeiffe DU, Dobson H: Influence of uterine bacterial contamination after parturition on ovarian dominant follicle selection and follicle growth and function in cattle. Reproduction, 123, 837-845, 2002. DOI: 10.1530/rep.0.1230837
41. Messier S, Higgins R, Couture Y, Morin M: Comparison of swabbing and biopsy for studying the flora of bovine uterus. Can Vet J, 25 (7): 283- 288, 1984.
42. Sheldon IM, Lewis GS, LeBlanc S, Gilbert RO: Defining postpartum uterine disease in cattle. Theriogenology, 65 (8): 1516-1530, 2006. DOI: 10.1016/j.theriogenology.2005.08.021
43. Maurer RR, Echternkamp SE: Repeat breeder females in bef cattle influences and causes. J Anim Sci, 61 (3): 625-636, 1985.
44. Miller HV, Kimsey PB, Kendrick JW, Darien B, Doering L: Endometritis of dairy cattle: Diagnosis, treatment and fertility. Bovine Pract, 15,13-23, 1980.
45. Shams-Esfandabadi N, Shirazi A, Ghasemzadeh-nava H: Pregnancy rate following post-insemination intrauterine treatment of endometritis in dairy cattle. J Vet Med, 51 (3): 155-156, 2004. DOI: 10.1111/j.1439- 0442.2004.00618.x
46. Gümen A, Yılmazbaş Mecitoğlu G, Keskin A, Karakaya E, Alkan A, Taşdemir U, Okut H: The effect of intrauterine cephapirin treatment after insemination on conception rate in repeat breeder dairy cows subjected to the progesterone-based Ovsynch protocol. Turk J Vet Anim Sci, 36 (6): 622-627, 2012. DOI: 10.3906/vet-1104-13
47. Mosaferi S, Badie AD, Nikniaz H: Effect of intrauterine antibiotic injection 24 hours after insemination on conception rate in cows with endometritis. Ann Biol Res, 4 (5): 312-315, 2013.
48. Ahmadi MR, Dehghan SA: Evaluation of the treatment of repeat breeder dairy cows with uterine lavage plus PGF2α, with and without cephapirin. Turk J Vet Anim Sci, 31 (2): 125-129, 2007.
49. Kumar R, Singh RK, Singh JB, Singh S: Clinical management of repeat breeding syndrome in bovines. Intas Polivet, 13 (I): 23-25, 2012.
50. Parikh SS, Savaliya BD, Makwana RB, Patbandha TK, Gajbhiye PU: Therapeutıc effıcacy of varıous intrauterıne drugs on repeat breeder Gır Cows. Int J Sci Environ Technol, 6 (3): 2107-2111, 2017.
51. Saili T, Baa LO, Napirah A, Syamsuddin, Sura IW and Lopulalan F: Pregnancy rate of Bali Cows following artificial insemination using chilled sexed sperm under intensive management in tropical area. The 7th International Seminar on Tropical Animal Production, At Yogyakarta, Indonesia. Contribution of Livestock Production on Food Sovereignty in Tropical Countries September 12-14, Proceedings, Yogyakarta, Indonesia, 2017.
52. Inskeep EK, Dailey RA: Embryonic death in cattle. Vet Clin North Am Food Anim Pract, 21 (2): 437-461, 2015. DOI: 10.1016/j.cvfa.2005.02.002
53. Barth AD: Factors affecting fertility with artificial insemination. Vet Clin North Am Food Anim Pract, 9 (2): 275-289, 1993. DOI: 10.1016/s0749- 0720(15)30646-0
54. García-Ispierto I, López-Gatius F, Santolaria P, Yániz JL, Nogareda C, López-Béjar M: Factors affecting the fertility of high producing dairy herds in northeastern Spain. Theriogenology, 67 (3): 632-638, 2007. DOI: 10.1016/j.theriogenology.2006.09.038
55. Arı UÇ, Pancarcı ŞM, Kaçar C, Güngör Ö, Lehimcioğlu NC, Öztürkler Y, Yıldız S: Effect of progestagen application during ovsynch protocol on pregnancy rates of lactating-grazing cows. Kafkas Univ Vet Fak Derg, 23 (2): 319-324, 2017. DOI: 10.9775/kvfd.2016.16522