Pathologic findings and immunohistochemical distribution of viral antigen in experimental avian encephalomyelitis of the chick and quail

Bu çalışmada, avian ensefalomyelitis virusunun van Roekel suşunun (referans suş) intraserebral ve intramuskuler yollarla inokule edildiği civciv ve bıldırcınlarda; patolojik ve immunohistokimyasal bulgulardaki farklılıklar ve tanıdaki önemleri karşılaştırmalı olarak incelendi. Histopatolojik incelemede, her iki türde perivasküler mononüklear hücre infiltrasyonları ve gliozisi içeren nonpurulent ensefalomyelitis tanımlandı. Civcivlerde nöronal lezyonlar, sentral kromatolizis ve nöronal büzüşme (nöronal piknoz) ile karakterize olurken, bıldırcınlarda ise yalnızca nöronal büzüşme görüldü. Viseral organlardan bezli mide, kaslı mide, bağırsaklar, dalak, karaciğer, pankreas, böbrekler, kalp, akciğerler ve gözlerde; lenfoid kümeler ve/ya da mononüklear hücre infiltrasyonları şeklinde yangısal yanıt gözlendi. Ancak, bıldırcınlarda benzeri yangısal reaksiyonlar bezli mide, bağırsaklar, kalp ve gözlerde sınırlı kalmıştı. Immunofloresan incelemede, viral antijen özellikle orta beyin, serebral hemisferler ve serebellum nöronlarında dikkati çekerken, medulla spinalis ve medulla oblongatada ise daha zayıftı. Immunreaksiyonun civcivlerin beyin ve medulla spinalislerinde, yangısal reaksiyonların şiddetiyle doğru orantılı olarak azaldığı dikkati çekti. Her iki türde viral antijen bezli mide, kaslı mide, bağırsaklar, kalp kası, pankreas, dalak, böbrekler, akciğerler ve gözde belirlendi.

Anahtar Kelimeler:

patoloji, civciv, deneysel enfeksiyon, immünohistokimya, kanatlı ensefalomiyelit virüsü, sinir sistemi hastalıkları, bıldırcın

Civciv ve bıldırcınların deneysel avian ensefalomyelitis’inde patolojik bulgular ve viral antijenin immunohistokimyasal dağılımı

In this study, the differences and diagnostic importances of the pathological and immunohistochemical findings in avian encephalomyelitis were investigated comparatively in chicks and quails by inoculating van Roekel strain (reference strain) via intracerebral and intramuscular routes. Histopathologically, nonpurulent encephalomyelitis characterized with infiltration of perivascular mononuclear cells and gliosis was described in both species. Neuronal lesion in the chicks consisted of central chromatolysis and neuronal shrinkage (neuronal pyknosis), whereas in quails it was composed of only neuronal shrinkage. Lymphoid aggregates and/or infiltration of mononuclear cells were observed in the proventriculus, gizzard, intestines, liver, pancreas, kidneys, myocardium, lungs, spleen and eyes. However, these lesions in quails were restricted only in the proventriculus, intestines, myocardium and eyes. In the immunofluorescein examination, viral antigen was found especially in neurons of the midbrain, cerebral hemispheres, cerebellum, and occasionally in medulla oblongata and medulla spinalis in both species. Viral antigen in the brain and spinal cord samples of the chicks decreased gradually by increasing inflammatory reaction. Viral antigen in both species was continously detected in the proventriculus, gizzard, intestines, myocardium, pancreas, spleen, liver, kidneys, lungs and eyes..

Keywords:

pathology, chicks, experimental infection, immunohistochemistry, avian encephalomyelitis virus, nervous system diseases, quails,

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