Sırrı AVKİ, Hülya TÜRÜTOĞLU, Rüçhan ALP, KÜRŞAD YİĞİTARSLAN, M. Doğa TEMİZSOYLU

Determination of Clostridium botulinum toxins in dairy cows with abomasal displacement

Clostridium botulinum toksikasyonunun yeni bir formu ‘visceral botulizm’ olarak tanımlanmaktadır. Botulinum toksininin uzun sürede ve çok düşük miktarlarda emilmesi, akut ya da sub-akut ölümcül patolojiler yerine, intestinal organların nörolojik kontrolünde bozukluklara neden olmaktadır. Sunulan çalışma, abomasum deplasmanı tanısı konmuş ineklerin serum ve abomasum içeriğinde C. botulinum toksinlerinin varlığını ve olası tiplerini belirlemek amacıyla yapıldı. Abomasum deplasmanı tanısı laparaskopik olarak doğrulanmış 17 inekten, cerrahi tedavi sırasında abomasum içeriği ve serum örnekleri toplandı. Örneklerde C. botulinum toksinlerinin varlığı ve tiplendirilmesi, fare toksisite ve nötralizasyon testleri ile yapıldı. Abomasum içeriği örneklerinden 2’sinde ve serum örneklerinden 1’inde C tipi C. botulinum toksini tespit edildi. Toksin belirlenen ineklerin silaj ile beslendiği belirlendi. C tipi toksin varlığının %17.6 oranında tespit edilmiş olması, abomasum deplasmanı gelişiminde C tipi C. botulinum toksininin etkili olduğu şeklinde yorumlanamaz. Ancak abomasum deplasmanı’nın multifakröriyel etiyolojili bir hastalık olduğu dikkate alınırsa bu toksinlerin olası varlığı göz ardı edilmemelidir.

Anahtar Kelimeler:

toksisite, Clostridium botulinum, abomasum, botulizm, inek, deplasman, nötralizasyon testi, toksinler, silaj

Abomasum deplasmanlı ineklerde Clostridium botulinum toksinlerinin araştırılması

A new form of bovine Clostridium botulinum toxicity was described as visceral botulism. Long-lasting absorption of very low quantities of botulinum toxin may interfere with neurological control of intestinal physiology instead of acute or subacute lethal effects. The objective of this study was to investigate the presence of C. botulinum toxins and their types in abomasal content and serum of dairy cows diagnosed with abomasal displacement. Abomasum displacement was confirmed in 17 cows by laparoscopic examination and abomasal content and serum samples were collected during surgical correction. The mouse toxicity and neutralization bioassay was used to detect and identify the C. botulinum toxins in samples. Clostridium botulinum toxin type C was detected in 2 of abomasums contents and in 1 of sera. The toxin detected animals were fed with silage. Although the identification ratio (17.6%) of C. botulinum type C toxin in this study seems so farther to correlate AD with C. botulinum toxins; we are of the opinion that C. botulinum toxins, especially type C, may be associated with AD because AD is considered to be a multifactorial disease.

Keywords:

toxicity, Clostridium botulinum, abomasum, botulism, cows, displacement, neutralization tests, toxins, silage,

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