Radyo frekans sinyalinin rat spinal ganglion hücrelerinde kalsiyum bağımlı klor kanallarına etkisi

Bu araştırma ile küresel mobil iletişim sistemlerinden (GSM: Global system for mobil communications) yayılan radyo frekans dalgalarının rat spinal ganglion sinir hücreleri hücre zarında bulunan kalsiyum bağımlı klor kanallarına etkisinin araştırılması amaçlanmıştır. Bu amaçla 20 adet 2 günlük Wistar sıçanlardan elde edilen ve kültür ortamında hazırlanan spinal ganglion sinir hücreleri kullanılmıştır. Hazırlanan çözelti içinde bulunan sinir hücreleri 5 santim mesafede 10 V/m elektrik alan şiddetinde 900 Mhz. frekansında toplam 6 dakika radyo frekansa maruz bırakıldı. Patch kenetleme tekniği ile hücre klor kanal akımlarında meydana gelen değişiklikler kaydedildi. Kayıtların analizinde ortama uygulanan radyo frekans sinyalinin, kalsiyum bağımlı klor kanallarının akımlarında ve membran potansiyelinde anlamlı değişimler oluşturmadığı gözlenmiştir.

Anahtar Kelimeler:

sinir dokusu, kalsiyum, klor, haberleşme, ganglion, sinir sistemi, sinir hücresi, sıçan

The effect of radio frequency signals on calcium dependent chlorur channels of spinal ganglial cells

This study is intended to predict the effect of radio frequency waves of global mobile communication systems (GSM: Global system for mobile communication systems) on the calcium dependent chlorur channels of rat dorsal root ganglia neural cell membranes. For this purpose the spinal ganglia cells from 20 Wistar rats which were 2 days old and those prepared in culture media were used. The nerve cells were radiated by 10V/m electric field strength at 900 Mhz. from a distance of 5 cm for six minutes. Changes in cell current were recorded by patch clamp techniques. In the analysis of the records the applied radio frequency signal was not found to change the currents of calcium dependent chlorur channels and the membrane potential meaningfully. The analysis of recordings does not show any meaningful change in the currents of calcium dependent chlorur channels and the membrane potential of the spinal ganglia cells after exposure to a 900 Mhz radiofrequency field.

Keywords:

nerve tissue, calcium, chlorine, communication, ganglia, nervous system, neurons, rats,

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