Pathologic changes and use of immunohistochemical methods in naturally occuring avian encephalomyelitis of the chicks

Çalışmada, üç kümesten sağlanan doğal enfekte civcivlerdeki avian ensefalomiyelitis (AE)’in patolojik ve immunohistokimyasal bulguları tanımlanır. Civcivlerde, ataksi, parezis, paralizis, tremor ve tortikollis göze çarpan klinik bulgulardı. Santral sinir sistemin (CNS)’de nöron dejenerasyonu ve nekrozlarını da içeren nonpurulent ensefalomiyelitis en dikkat çekici histopatolojik bulgulardı. Viseral organlardaki değişiklikler; lenfoid kümeler ve/ya da mononuklear hücre infiltrasyonlarından oluşmaktaydı. Viral antijeni belirlemede; formalin tespitli parafin kesitlere avidin-biotin peroksidaz kompleks (ABC) ve immunofloresan (IF) metotlar uygulandı ve sonuçları karşılaştırmalı olarak değerlendirildi. Her iki testte de, AE viral antijen pozitifliği CNS, bağırsaklar, bezli mide, böbrekler, akciğerlerde ve kalpte şiddetli iken; kaslı mide, karaciğer, pankreas, dalak, gözler ve Bursa fabrisius’ta ise zayıftı. Her iki metot, immunreaksiyonların şiddeti ve dokulardaki dağılımı yönünden karşılaştırıldığında, ABC metot IF’ye göre daha duyarlı idi.

Anahtar Kelimeler:

dokular, peroksidaz, patoloji, bağışıklık bilimi teknikleri, immünohistokimya, immünofloresan, kanatlı ensefalomiyelit virüsü, avidin, biyotin antogonist, civciv, ensefalit

Civcivlerin doğal avian ensefalomyelitis’inde patolojik değişiklikler ve immunohistokimyasal metotların uygulanması

The present study describes the pathological and immunohistochemical findings of avian encephalomyelitis (AE) in naturally infected 31 chicks obtained from three broiler rearing farms. Clinically, ataxia, paresis, paralysis, tremor and torticollis were observed in the chicks. Nonpurulent encephalomyelitis accompanied by neuronal degeneration and necrosis was the most pronounced histopathological findings of the central nervous system (CNS). Lymphoid aggregations and/or mononuclear cell infiltrations were observed in the visceral organs. The avidin-biotin peroxidase complex (ABC) and immunofluorescence (IF) methods were compared for AE viral antigen detection in formalin-fixed, paraffin embedded tissues. With the both tests, condensed viral immunolabelling was detected particularly in the CNS, intestines, proventriculus, kidneys, lungs and heart and, to a lesser extend, in the gizzard, liver, pancreas, spleen, eyes and fabricius of Bursa. Compared of both methods according to labelling intensity and distribution of the viral antigen, ABC method was found to be more sensitive than IF method.

Keywords:

tissues, peroxidase, pathology, immunological techniques, immunohistochemistry, immunofluorescence, avian encephalomyelitis virus, avidin, biotin antagonists, chicks, encephalitis,

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