HARUN ÜLGER, SAİM ÖZDAMAR, ERDOĞAN UNUR, Margaret PRATTEN

The effect of basic fibroblast Growth factor (bFGF) on in vitro embryonic Growth, heart and neural tube development in rat

Basic Fibroblast Growth Faktörün (bFGF) neural tüp, kalp ve total embriyonik gelişim üzerine etkisi, total rat serumunda (WRS), 30 kilodalton’un (kDa) altındaki serum fraksiyonu filtre edilen serumda [retenate (R)] ve R+bFGF’de kültüre edilen 9.5 günlük 60 embriyoda (yedi hamile rattan elde edilen) incelendi. 48 saatlik kültürden sonra her gruba ait embriyolar tekrar iki gruba bölündü. Birinci grup embriyolar morfolojik incelemeye ve protein içeriğini tespit etmek için biyokimyasal analize tabi tutulurken, ikinci gruptan seri kesitler alınarak ışık mikroskobunda incelendi. Morfolojik skor, embriyo protein içeriği, somit sayısı ve baş-kıç uzunluğu, R’de kültüre edilen embriyolarda gerileme gösterirken, bFGF ilaveli R’de kültüre edilen embriyolarda embriyonik büyüme ve gelişmenin arttığı görüldü. WRS, R ve R+bFGF’ de kültüre edilen embriyoların morfolojik skorları sırasıyla 57.7±0.87, 46.6±1.90 ve 52.1±0.97, somit sayıları 26.5±0.47, 20.1±0.63 ve 24.4±0.46, baş-kıç uzunlukları 3±0.07, 2.4±0.06 ve 2.7±0.06 mm ve protein içerikleri 160.5±7.41, 98.2±4.81 ve 141.1±10.96 mg olarak tespit edildi. Histolojik sonuçlar R’de kültüre edilen embriyoların kalplerinde bölünmenin olmadığı ve tübüler yapısını koruduğunu ortaya koydu. Bu embriyolar ayrıca cranial ve caudal açıklığı bulunan neural tüp deffeklerine sahipti. R’ye bFGF ilavesi kalp gelişimini düzeltti. WRS ve R+bFGF grubunda septum interventriculare’ye ait kas gelişimi başlamıştı. Hem morfolojik analiz ve hem de histolojik kesitler bFGF’in kalp ve neural tüp gelişimi üzerinde etkin olduğunu ortaya koymaktadır.

Anahtar Kelimeler:

iyileşme, anormallikler, biyolojik gelişim, defekt, embriyonik gelişim, fibroblast, büyüme faktörleri, kalp, doku bilimi, morfoloji, sıçan

Basic Fibroblast Growth faktörün (bFGF) ratlarda in vitro embriyonik büyüme, kalp ve neural tüp gelişimi üzerine etkisi

In vitro effects of bFGF on neural tube, heart, and total embryonic development were investigated in 60 rat embryos (obtained from seven pregnant females) at 9.5 days of gestation that were cultured in whole rat serum (WRS), in >30 kDa serum fractions [retenate (R)], and in R+bFGF. After 48 h culture, the embryos from each group were harvested and divided into two groups. One group was analysed morphologically and biochemically to obtain embryo protein content, the second group was serially sectioned and examined by light microscopy. Morphological score, embryo protein content, somite number and crown-rump length of embryos indicated that embryos cultured in R had significant embryonic retardation, whereas the addition of bFGF to R increased embryonic growth and development. The morphological scores for WRS, R and R+bFGF were 57.7±0.87, 46.6±1.90 and 52.1±0.97, somite numbers were 26.5±0.47, 20.1±0.63 and 24.4±0.46, crown-rump lengths were 3±0.07, 2.4±0.06 and 2.7±0.06 mm, and embryo protein contents were 160.5±7.41, 98.2±4.81 and 141.1±10.96 mg per embryo, respectively. The results of histological examination showed that the hearts of embryos grown in R were unseptated and tubular. These embryos also have open cranial and caudal neural tube defects. The addition of bFGF to R improved heart development. In WRS and R+bFGF groups, development of the muscular interventricular septum had begun. Both the morphological analyse and histological sections showed that the bFGF caused improved growth in heart and neural tube formation.

Keywords:

healing, abnormalities, biological development, defects, embryonic development, fibroblasts, growth factors, heart, histology, morphology, rats,

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