Mohammad NARIMANI RAD, Vahab BABAPOUR, Morteza ZENDEHDEL, Mehran MESGARİ ABBASİ, Sara FARHANG

Investigation on serum hormonal parameters (Ghrelin, Corticosterone, Insulin, T3 and T4) in chronic mild stress rat model of depression

Bu çalışmada, depresyon etkilerinin kronik hafif stres (CMS) işlemi ile stres ve depresif bozukluklarla etkileşim halinde olan serum hormonları (ghrelin, kortikosteron, insülin, T3 ve T 4) üzerine etkilerinin araştırılması amaçlanmıştır. Kırk beş adet 40-45 günlük laboratuvar sıçanları kontrol (n: 10) ve CMS (CMS işlemine tabi, n: 30) olmak üzere iki gruba ayrıldı. Kontrol grubundaki sıçanlar herhangi bir çevresel stres olmadan tek bir kafeste yetiştirildi. CMS grubu sıçanlar CMS işlemine dahil edildi. Bu protokol hafif öngörülemeyen stres oluşturucularının (kesintili aydınlatma, stroboskopik ışık, gruplama, yiyecek veya su yoksunluğu, boş bir su şişesine, katı kafese, kafes eğimine maruziyet, vb) oluşmaktadır. CMS işleminden sonra sükroz tercih (SP) testi depresif hayvanların tanımlanması için kullanıldı. %65 SPden daha düşük sıçanlar depresif hayvan olarak tanımlandı. Kan serumu ELISA kitleri ile kan hormonsal değişkenlerin tespiti için iki grutan (kontrol ve CMS) alındı. Analiz edilen sonuçlar kontrol grubu ile karşılaştırıldığında çok önemli kortikosteron artışıyla beraber CMS grupta (depresif hayvanlar) ghrelin, kortikosteron ve insülin seviyelerindeki önemli artışı gösterdi. CMS gruplarında kontrol grubu ile karşılaştırıldığında düşmüş T4e rağmen T3 için önemli bir değişiklik gözlemlenmedi. Kronik hafif stres kaynaklı depresyonun ghrelin, kortikosteroid ve insülin artışına ve T4 azalmasına sebep olabileceği sonucuna varıldı. Hormonsal endeks üzerindeki (tiroit hormonları hariç) CMS kaynaklı depresyonun adı geçen etkileri majör depresyon etkileri ile benzer olabilir.

Depresyonun kronik hafif stres sıçan modelinde serum hormonal parametrelerin (Ghrelin, Kortikosteron, İnsülin, T ve T) araştırılması

The aim of present study was to investigate the effects of depression induced with chronic mild stress (CMS) protocol on serum hormones (ghrelin, corticosterone, insulin, T3 and T4) which is in interaction with stress or depressive disorders. Forty-five 40-45 d old laboratory rats were assigned to two groups; control (n: 10) and CMS (subjected to CMS procedure, n: 30). Rats in the control group were reared in single cages without any environmental stress. Rats in CMS were entered the CMS procedure. This protocol consisted of mild unpredictable stressors (intermittent illumination, stroboscopic light, grouping, food or water deprivation, exposure to an empty water bottle, solid cage, cage tilting, etc.). After CMS protocol sucrose preference (SP) test was used for the identification of depressed animals. Rats with lower than 65% SP were defined as depressed animal. Blood serum was taken from two groups (control and CMS) for the determination of blood hormonal variables by Elisa kits. Analyzed data showed a significant elevation in ghrelin, corticosterone, and insulin levels for CMS group (depressed animals) in comparison with control group, as elevation was very significant for corticosterone. There was not any significant change for T3, whereas decreased T4 was observed for CMS groups when compared with the control. It was concluded that chronic mild stress induced depression can cause ghrelin, corticosteroid, and insulin increases, and T4 decreases. Mentioned effects of CMS induced depression on hormonal indices (with exception to thyroid hormones), can be similar with effects of major depression.

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