Adem KARA, Deniz TEKİNER, Nejdet ŞİMŞEK, HÜLYA BALKAYA, ZEKERİYA ÖZÜDOĞRU

Distribution and location of endocrine cells in the pancreas of the sparrowhawk, accipiter nisus

Bu çalışmada, atmaca pankreasının dorsal, ventral ve splenik loblarında bulunan endokrin hücrelerin dağılımı immun- histokimysal metotlarla belirlenmiştir. Pankreas dokusu anti-glukagon, anti-insulin, anti-gastrin ve anti-somatostatin primer antikorları kullanılarak streptavidin-biotin peroxidase metodu ile boyandı. Çalışmada, glukagon (alfa hücresi), insülin (beta hücresi) ve somatostatin (delta hücresi) salgılayan hücrelerin splenik lobda sayısının en fazla olduğu saptandı. Atmalarda pankreasın endokrin bölümlerinin alfa, beta ve miks adacıklar halinde gruplaştıkları saptandı. Beta adacıklarının çok sayıda beta hücresi, az sayıda deltaalfa hücrelerini içerdiği belirlendi. Alfa adacıkları, genellikle alfa ve delta hücrelerinden nadiren de beta hücrelerinden oluşuyordu. Ayrıca, gastrin pozitif hücreler, sadece ekzokrin pankreasta 1 ya da 2-3 adet hücre grubu halinde bulunurken, seratonin pozitif hücrelere ise ekzokrin ve endokrin pankreasın herhangi bir bölümünde rastlanmadı. Sonuç olarak, atmaca pankreasının lop sayısı, endokrin adacık tipleri ve endokrin hücre lokalizasyonu açısından etçil kuşlarla benzerliği, evcil kuş türleriyle de farklılıkları belirlenmiştir.

Atmaca (Accipiter nisus) pankreas dokusu endokrin hücrelerin dağılımı ve lokalizasyonu

The distribution and location of endocrine cells were determined in the dorsal, ventral, and splenic lobes of sparrowhawk pancreas using immunohistochemical methods. In this study, pancreatic tissues were stained with anti-insulin, anti-glucagon, anti-somatostatin, nti-gastrin, and anti-serotonin primary antibodies using the streptavidin-biotin-peroxidase method. The results showed thatnumbers of glucagon- (alpha cell), insulin- (beta cell), and somatostatin- (delta cell) releasing cells were high and located in the splenic obe of the pancreas. These endocrine cells were grouped into alpha, beta, and mixed islets. Alpha islets were mainly composed of alpha nd delta cells and also occasionally beta cells. Beta islets contained numerous beta cells and a few delta and alpha cells. Furthermore, n the exocrine tissue were showed as only one cell or 2-3 gastrin immunopositive cell groups, whereas serotonin immunopositive cells were not found anywhere in the exocrine and endocrine pancreas. In conclusion, the endocrine islet types, endocrine cell localizations nd lobe numbers of pancreas in sparrowhawks are similar to predator bird species, but are determined different to domestic fowls.

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