SEVİL ERDENLİĞ GÜRBİLEK, E. Ayhan BALKAN, H. Yavuz AKSOY, Judy STACK

Comparative production of rapid slide agglutination test (RSAT) antigen used in serological diagnosis of brucella canis in different culture media in the fermenter

Bu çalışmada Brucella canis infeksiyonunun çabuk, pratik ve ekonomik teşhisi için homolog ve heterolog suşlar ile hazırlanmış çabuk lam aglütinasyon test (ÇLAT) antijenleri üretildi. Antijen üretiminde homolog suşlar (B. canis RM6/66 suşu ve saha B. canis suşu) ve heterolog suşlar (B. abortus 45/20 ve B. melitensis B115) kullanıldı. Bu amaçla çalışmada kullanılacak test suşları, tripton sıvı besi yeri (TSB), brusella broth besiyeri (BBB) ve brain heart infüzyon broth besiyeri (BHIB) olmak üzere 3 ayrı besi yerinde optimal üremeyi değerlendirmek üzere karşılaştırmalı olarak üretildiler. Test besiyerlerinde en iyi üremeyi B. canis RM6/66 suşu ve en zayıf üremeyi B. melitensis B 115 suşu gösterdi. Suşların en iyi üredikleri besi yerleri TSB oldu. Sonuçta tüm test suşlarından hazırlanan ÇLAT antijenleri başarılı bir şekilde üretilip standardize edildiler. Test sonuçlarına göre B. canis RM6/66 ve/veya saha B. canis suşlarının ticari ÇLAT antijeni üretimi için seçilebileceği düşünüldü. Sonuç olarak üretilen ÇLAT antijeninin Türkiyede köpeklerde, yaygın olarak rutin klinik muayenenin bir parçası olarak hastalığın serolojik teşhisinde kullanılabileceği ve bu testin insanlardaki klasik bruselloz tanısına dahil edilmesinin, hastalığın insanlardaki durumunun daha sağlıklı olarak değerlendirilmesine olanak sağlayacağı sonucuna varıldı.

Brucella canisin serolojik tanısında kullanılan çabuk lam aglütinasyon test (ÇLAT) antijeninin fermentörde farklı nesiyerlerinde karşılaştırmalı olarak üretimi

In this study, rapid slide agglutination test (RSAT) antigens were produced by using homologous (Brucella canis RM6/66 and a wild B. canis) and heterologous brucella strains (B. abortus 45/20 and B. melitensis B115) for a quick, practical and economic diagnosis of B. canis infection in dogs and humans. All the test strains were grown in three different culture media, namely, trypton liquid media (TLM), brucella broth media (BBM) and brain heart infusion broth media (BHIBM) to compare their optimal growths. The best growth rate was obtained by B. canis RM6/66 strain. B. melitensis B 115 showed the poorest growth in all test media. The culture media that supported the best growth rate in all tested strains was TLM. RSAT antigens from each test strains were produced without any stringy formation and standardised successfully. It was thought that B. canis RM6/66 and/or wild B. canis might be suitable candidate in commercial RSAT antigen production according to the test results supporting the fact that using homotypic strain increases the diagnostic sensitivity. As a conclusion, it was decided that this antigen will be able to used extensively as a part of routine clinical examination in dogs in Turkey. Furthermore, it was considered that to include RSAT in humans as a part of routine brucellosis diagnosis could help to evaluate the disease more accurately.

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