Biochemical profile of paenibacillus iarvae repetitive element polymerase chain reaction (rep-PCR) genotypes in Bulgaria

Bu çalışmanın amacı Bulgaristanda Paenibacillus larvae repetitive element polymerase chain reaction (rep-PCR) genotiplerini belirlemek ve genotip ile fenotip arasındaki ilişkiyi ortaya koymaktır. Toplam 103 izolat (genotip AB, n=21; genotip ab, n=82) ve eferans suş olarak NBIMCC 8478 BioLog Gen III identifikasyon sistemi kullanılarak ve nitrat indirgeme kabiliyeti yönünden analiz edildi. Genotip AB ve ab, BioLog system ile 71 karbon temelli özel bir metabolik parmak izi gösterdi. Nitrat indirgeme kabiliyeti, mannitol ve alisin kullanımı göz önüne alınarak suşlar biyotip I, III, IV ve VIII olarak ayrıldı. Paenibacillus larvae АВnin büyük bölümü biyotip IIIde oplanırken ab çoğunlukla biyotip Ide gruplandı. Biyotip I ve IV test edilen AB suşları arasında tespit edilmedi. Bu çalışma rep-PCR genotipleri AB/ab ile biyokimyasal fenotipler arasındaki ilişki koyarak epidemiyolojik çalışmalarda enfeksiyon kaynağını tespit etme ve hastalığın kontrolünde faydalı olacaktır.

Bulgaristanda paenibacillus iarvae repetitive element polymerase chain reaction (rep-PCR) genotiplerinin biyokimyasal profili

The aim of the present study was to determine the biochemical profile of Paenibacillus larvae repetitive element polymerase chain reaction (rep-PCR) genotypes in Bulgaria and to assess the link between genotype and phenotype. A total of 103 isolates (genotype AB, n=21; genotype ab, n=82) and a reference strain NBIMCC 8478 were analyzed using identification system BioLog Gen III and nitrate reducing ability. Genotypes AB and ab showed a particular metabolic fingerprint based on 71 carbon sources provided by BioLog system. Considering the nitrate reducing ability, mannitol and salicin utilization, the strains were distributed into biotypes I, III, IV and VIII. The majority of Paenibacillus larvae АВ clustered into biotype III while ab were grouped mainly into biotype I. Biotypes I and IV were not found among the tested AB strains. This study showed the obvious link between rep-PCR genotypes AB/ab and biochemical phenotype that can be useful in epidemiologic situations to trace the source of infection and to control the disease.

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