Tiwaporn RADEEROM, Kriangkrai THONGKORN, Kittisak BUDDHACHAT, Waranee PRADİT, Siriwadee CHOMDEJ, Puntita SIENGDEE, Korakot NGANVONGPANIT

Investigation of the Calculus Microbiome in Canines and Felines Using Next-Generation Sequencing

The oral cavity of dogs and cats is colonized by hundreds of bacterial species. Here, we describe the bacterial composition in the dental calculus of dogs and cats. Dental calculus samples from 43 dogs and 4 cats were pooled into four different groups. Dogs were categorized into three groups: non-small breed dogs (NSB), non-brachycephalic small breed dogs (SB) and brachycephalic small breed dogs (SBb). The fourth group included cats. Bacterial communities were identified based on 16S rRNA sequencing (V3 and V4 hypervariable regions) with the Illumina platform. The numbers of operational taxonomic units (OTUs) identified in the three groups of dogs were 180, 190 and 150 and in NSB, SBb and SB, respectively, while in cats there were 111 OTUs. In dental calculus from both dogs and cats, the phylum Firmicutes had the highest proportion of read number, especially the class Clostridia. PCoA and UPGMA analysis revealed differences in the microbiomes of canine and feline calculus. Our findings demonstrated that the bacterial communities in calculus seemed to differ from those in other sites of the oral cavity. Calculus may serve as a potential habitßat for the growth of bacteria linked to canine and feline periodontal disease.

Köpek ve Kedi Diş Taşı Mikrobiyomunun İleri Jenerasyon Sekanslama Kullanılarak Araştırılması

Köpek ve kedilerin ağız boşluğu yüzlerce bakteri türü tarafından kolonize edilmiştir. Bu çalışmada; köpek ve kedilerin diş taşlarının bakteriyal kompozisyonu tanımlanmıştır. 43 köpek ve 4 kediye ait diş taşı örnekleri dört faklı grupta toplandı. Köpekler üç grupta kategorize edildi: küçük olmayan ırk köpekler, brakisefalik olmayan küçük ırk köpekler ve brakisefalik küçük ırk köpekler. Dördüncü grup ise kedileri içermekteydi. Bakteriler Illumina platform kullanılarak 16S rRNA sekanslama (V3 ve V4 çokdeğişken bölgeler) temeline göre belirlendi. Üç grup köpekte operasyonel taksonomik birimlerin sayısı küçük olmayan ırk köpekler, brakisefalik küçük ırk köpekler ve brakisefalik olmayan küçük ırk köpekler için sırasıyla 180, 190 ve 150 olarak tespit edilirken kedilerde 111 olarak belirlendi. Hem köpek hem de kedi diş taşlarında, Firmicutes filumu, özellikle de Clostridia sınıfı, en fazla okuma sayısına sahipti. PCoA ve UPGMA analizi köpek ve kedi diş taşlarının mikrobiyomu arasında farklılık olduğunu gösterdi. Elde edilen sonuçlar diş taşlarındaki bakteriyal topluluklarının ağız boşluğunun diğer taraflarından belirlenenlerden farklı olduğunu gösterdi. Diş taşları köpek ve kedilerde periodontal hastalıklar ile ilişkili bakterilerin büyümesi için uygun bir ortam oluşturabilir.

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