Bir İnfeksiyöz Koyun Keratokonjonktivit Salgınından Moraxella ovis ve Mycoplasma conjunctivae İzolasyonu ve Güçlendirilmiş Tedavi Yaklaşımı
Bu çalışmada, semi-entansif olarak yetiştirilen bir Tuj koyun sürüsünde doğal olarak gözlenen bir infeksiyöz keratokonjonktivit (IKC) salgınının etyolojisinin belirlenmesi ve semptom gösteren koyunlarda kuvvetlendirilmiş tedavi seçeneklerinin denenmesi amaçlanmıştır. Klinik keratokonjunktivitis belirtisi olan 42 koyunun konjunktival örnekleri, steril sıvap kullanılarak aseptik koşullarda toplandı. Tüm örnekler bakteri izolasyonu için kültüre edildi. Tedavi seçeneklerini test etmek için ergin koyunlardan oluşan semptomatik hayvanlar rastgele 3 gruba ayrıldı. Birinci grupta (n=13) penisilin G potasyum’un (250.000 IU/hayvan) subkonjunktival uygulanarak, ikinci grupta (n=14) oksitetrasiklin ve polimiksin B sülfat’ın gözkapağı içine mercimek tanesi büyüklüğünde konularak ve üçüncü grupta (n=15) ise amikasin sülfat, seftriakson disodyum (0.5 g) ve flukonazol (2 mg/mL) kombinasyonu damlatma şeklinde uygulanarak tedavi denemeleri yapıldı. Bakteriyolojik muayene sonucunda, 42 konjunktival sıvap örneğinin 36’sında (%85.71) Moraxella ovis ve bu pozitif örneklerin 3’ünde (%7.14) Mycoplasma conjunctivae izole edildi. Tedavi sonrası, birinci grubun %84.61’sı (11/13); ikinci grubun %71.42’si (10/14) ve üçüncü grubun ise tümünde iyileşme görüldü. Bu çalışma, M. ovis’in Tuj koyunlarında IKC’ye neden olan primer patojen, M. conjunctivae’nın ise sekonder etken olduğunu göstermiştir. Amikasin sülfat, seftriakson disodyum ve flukonazol kombinasyonu, koyunlarda IKC’nin en etkili tedavi seçeneği olarak tanımlanmıştır.
Moraxella ovis and Mycoplasma conjunctivae Isolation from an Ovine Infectious Keratoconjunctivitis Outbreak and Fortified Treatment Approaches
The aim of this study was to determine the etiology of naturally occurring infectious keratoconjunctivitis (IKC) outbreak observed in a Tujsheep herd rearing semi-intensively and to try fortified treatment options in symptomatic sheeps. Conjunctival samples from 42 sheepwith keratoconjunctivitis were collected aseptically using sterile cotton swabs. All swab were cultivated for bacterial isolation. To testtreatment options symptomatic animals were randomly divided into 3 groups each were composed of adult ewes. The first group (n=13)was treated with the subconjunctival administration of penicillin G potassium (250.000 IU/animal), the second group (n=14) was treated withoxytetracycline and polymyxin B sulfate, and the third group (n=15) was treated with amikacin sulfate (diluted with isotonic NaCl to 25 mgper mL), ceftriaxone disodium and fluconazole (2 mg/mL) combination. As the results of the bacteriological examination, 36 (85.71%) of 42conjunctival samples were found positive for Moraxella ovis. Also, Mycoplasma conjunctivae was identified in 3 (7.14%) conjunctival samplespositive for M. ovis. After treatment, 84.61% (11/13) of the first group; 71.42% (10/14) of the second group and all animals in the third groupwere observed to be healed. This study demonstrated that M. ovis was the primary pathogen causing IKC in Tuj sheeps and M. conjunctivaewas the second rank. The combination of amikacin sulfate, ceftriaxone disodium and fluconazole have been identified as the most effectivetreatment option of IKC in sheep.
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- Alexander D: Infectious bovine keratoconjunctivitis: A review of cases
in clinical practice. Vet Clin North Am: Food Anim Pract, 26, 487-503, 2010.
DOI: 10.1016/j.cvfa.2010.09.006
- Rankin GO, Sutherland CH: Nephrotoxicity of aminoglycosides and
cephalosporins in combination. Adverse Drug React Acute Poisoning Rev, 8
(2): 73-88, 1989.
- Loy JD, Brodersen BW: Moraxella spp. isolated from field outbreaks
of infectious bovine keratoconjunctivitis: A retrospective study of case
submissions from 2010 to 2013. J Vet Diagn Invest, 26 (6): 761-768, 2014. DOI:
10.1177/1040638714551403
- Egwu GO: In vitro antibiotic sensitivity of Mycoplasma conjunctivae and
some bacterial species causing ovine infectious kerato-conjunctivitis. Small
Rumin Res, 7, 85-92, 1992. DOI: 10.1016/0921-4488(92)90071-B
- Chapman HM, Murdoch FR, Robertson ID, Beatty DT: Detection,
identification and treatment of infectious ovine keratoconjunctivitis (Pink
Eye) in sheep from a Western Australian pre-export feedlot. Meat & Livestock
Australia, North Sydney, Australia, 2010.
- 39. Abdou NEMI, Abdou MY: Bacterial conjunctivitis in cattle and antibiotic
sensitivity of the isolates. Res J Anim Vet Sci, 5, 38-42, 2010.
- 38. Kibar M, Gümüşsoy KS, Öztürk A: Evaluation of various antibiotic
treatments in calves with ınfectious bovine keratoconjunctivitis. Turk J Vet
Anim Sci, 30, 553-559, 2006.
- Gökçe I, Çitil M, Genç O, Erdoğan HM, Güneş V, Kankavi O:
Comparision of the efficacy of florfenicol and oxytetracycline in the
treatment of naturally occurring infectious bovine keratoconjunctivitis. Ir
Vet J, 55 (11): 573-576, 2002.
- Işık N: Investigation on isolation and sensitivity of antibiotic on Morexella
bovis in cattle with infectious bovine keratoconjuctivitis. Dicle Üniv Vet Fak
Derg, 1, 17-22, 2008.
- Samsar E, Akın F, Gökçe P, Bilir B: Sığırların İnfeksiyöz Keratokonjuktivitisinde
subkonjuktival antibiyotik ve alfakimotripsin uygulamaları.
Ankara Üniv Vet Fak Derg, 40 (4): 453-474, 1993.
- Erdeğer J, Aydın N: Sığırlarda izole edilen Moraxella bovis suşlarının
çeşitli özelliklerinin araştırılması. Turk J Vet Anim Sci, 15, 140-147, 1991.
- Vaid RK, Anand T, Bera BC, Shukla BN, Nagar DK, Singh G, Virmani
N, Barua S, Singh K, Singh NK: Isolation and molecular identification of
Moraxella ovis and Moraxella spp. from Infectious Keratoconjunctivitis in
sheep in India. J Veterinar Sci Technol, DOI: 10.4172/2157-7579.S1.006
- Motha MXJ, Frey J, Hansen MF, Jamaludin R, Tham KM: Detection
of Mycoplasma conjunctivae in sheep affected with conjunctivitis and
infectious keratoconjunctivitis. N Z Vet J, 51 (4): 186-190, 2003. DOI:
10.1080/00480169.2003.36362
- Taghavi Razavizadeh AR, Razmyar J: Molecular diagnosis of Mycoplasma
conjunctivae in an outbreak of infectious keratoconjunctivitis in sheep. IJVR,
15 (1): 72-74, 2014. DOI: 10.22099/IJVR.2014.1988
- Shahzad W, Munir R, Rana MY, Ahmad R, Khan MS, Akbar G, Ijaz M,
Mehmood F: Prevalence, molecular diagnosis and treatment of Mycoplasma
conjunctivae isolated from infectious keratoconjunctivitis affected Lohi
sheep maintained at Livestock Experiment Station, Bahadurnagar, Okara,
Pakistan. Trop Anim Health Prod, 45 (3): 737-742, 2013. DOI: 10.1007/s11250-
012-0282-2
- Karthik K, Manimaran K, Mahaprabhu R, Shoba K: Isolation of
Moraxella sp. from cases of keratoconjunctivitis in an organized sheep farm
of India. Open J Vet Med, 7, 138-143, 2017. DOI: 10.4236/ojvm.2017.710014
- Elad D, Yeruham I, Bernstein M: Moraxella ovis in cases of infectious
bovine keratoconjunctivitis (IBK) in Israel. Zentralbl Veterinarmed B, 35, 431-
434, 1988. DOI: 10.1111/j.1439-0450.1988.tb00516.x
- Scott PR: Sheep Medicine. 265-270, London, UK, The Veterinary Press,
Manson Publishing, 2007.
- Kalkan Akçay E, Açıkgöz ZC, Can ME, Çelikbilek N, Dereli Can G, Çağıl
N: Fungal keratitis caused by Seedosporium apiospermum: First report from
Turkey. Mikrobiyol Bul, 47 (4): 727-733, 2013. DOI: 10.5578/mb.5262
- Hu FR, Huang WJ, Huang SF: Clinicopathologic study of satellite lesions
in nontuberculous mycobacterial keratitis. Jpn J Ophthalmol, 42 (2): 115-118,
1998. DOI: 10.1016/S0021-5155(97)00117-2
- Quinn PJ, Markey BK, Carter ME, Donnelly WJ, Leonard FC: Veterinary
Microbiology and Microbial Disease. 536, Iowa State University Press,
Ames, 2002.
- Barile MF, Del Giudice RA, Tully JG: Isolation and characterization
of Mycoplasma conjunctivae sp. n. from sheep and goats with keratoconjunctivitis.
Infect Immun, 5 (1): 70-76, 1972.
- Gupta B: Mycoplasma capricolum: Isolation and molecular diagnosis.
MSc. Thesis submitted to the U. P. Pt. Deen Dayal Upadhyaya Pashu-chikitsa
Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan, Mathura (U. P.). pp.
12-44, 2005.
- Baas EJ, Trotter SL, Franklin RM, Barile MF: Epidemic caprine
keratoconjunctivitis: Recovery of Mycoplasma conjunctivae and its possible
role in pathogenesis. Infect Immun, 18 (3): 806-815, 1977.
- Maboni G, Gressler LT, Espindola JP, Schwab M, Tasca C, Potter
L, Vargas AC: Differences in the antimicrobial susceptibility profiles of
Moraxella bovis, M. bovoculi and M. ovis. Braz J Microbiol, 46 (2): 545-549,
2015. DOI: 10.1590/S1517-838246220140058
- O’Brien TP: Management of bacterial keratitis: Beyond exorcism towards
consideration of organism and host factors. Eye, 17 (8): 957-974, 2003. DOI:
10.1038/sj.eye.6700635
- Shiun XW, Lo XJ, Ogawa-Lin: Effectiveness of povidone–iodine to treat
infectious conjunctivitis in dogs. RECVET, 3 (1): 1-5, 2008.
- Tümbay E: Sistemik etkili yeni bir antifungal kemoterapötik: Flukonazol.
Turk J Infect, 3 (1): 1989.
- Savani DV, Perfect JR, Cobo LM, Durack DT: Penetration of new
azole compounds into the eye and efficacy in experimental Candida
endophthalmitis. Antimicrob Agents Chemother, 31, 6-10, 1987. DOI: 10.1128/
AAC.31.1.6
- Köse S, Pamukçu K, Akkın C, Yağcı A, Tümbay E: Fungal keratitlerde
sistemik flukonazol tedavisinin etkinliği. T Oft Gaz, 23, 380-3, 1993.
- Behrens-Baumann W, Klinge B, Rüshel R: Topical fluconazole for
experimental Candida keratitis in rabbits. Br J Ophthalmol, 74, 40-42, 1990.
DOI: 10.1136/bjo.74.1.40
- Chiquet C, Romanet JP: Prescribing fortified eye drops. J Fr Ophtalmo,
30 (4): 423-430, 2007.
- Panda A, Ahuja R, Sastry SS: Comparison of topical 0.3% ofloxacin with
fortified tobramycin plus cefazolin in the treatment of bacterial keratitis. Eye,
13, 744-747, 1999. DOI: 10.1038/eye.1999.220
- O’Brien TP, Maguire MG, Fink NE, Alfonso E, McDonnell P: Efficacy of
ofloxacin vs cefazolin and tobramycin in the therapy for bacterial keratitis.
Arch Ophthalmol, 113 (10): 1257-1265, 1995. DOI: 10.1001 archopht.1995.
01100100045026
- Hyndiuk RA, Eiferman RA, Caldwell DR, Rosenwasser GO, Santos
CI, Katz HR, Badrinath SS, Reddy MK, Adenis JP, Klauss V: Comparison
of ciprofloxacin ophthalmic solution 0.3% to fortified tobramycin cefazolin
in treating bacterial corneal ulcers. Ophthalmology, 103 (11): 1854-1863,
1996. DOI: 10.1016/S0161-6420(96)30416-8
- Smith MC, Sherman DM: Goat Medicine, 2nd ed., June 2009, WileyBlackwell,
2011.
- O’Connor AM, Shen HG, Wang C, Opriessnig T: Descriptive epidemiology
of Moraxella bovis, Moraxella bovoculi and Moraxella ovis in beef calves with
naturally occurring infectious bovine keratoconjunctivitis (Pinkeye). Vet
Microbiol, 155 (2-4): 374-380, 2012. DOI: 10.1016/j.vetmic.2011.09.011
- Bath GF, Van Wyk JA, Pettey KP: Control measures for some important
and unusual goat diseases in southern Africa. Small Rumin Res, 60 (1-2): 127-
140, 2005. DOI: 10.1016/j.smallrumres.2005.06.007
- Giacometti M, Janovsky M, Belloy L, Frey J: Infectious keratoconjunctivitis
of ibex, chamois and other Caprinae. Rev Sci Tech, 21, 335-345,
2002.
- Dagnall GJR: The role of Branhamella ovis, Mycoplasma conjunctivae and
Chlamydia psittaci in conjunctivitis of sheep. Br Vet J, 150 (1): 65-71, 1994.
DOI: 10.1016/S0007-1935(05)80097-1
- Fernandez-Aguilar X, Rossi L, Cabezón O, Giorgino A, Llopis IV, Frey
J, López-Olvera JR: Infectious keratoconjunctivitis and occurrence of
Mycoplasma conjunctivae and Chlamydiaceae in small domestic ruminants
from Central Karakoram, Pakistan. Vet Rec,181 (9): 1-7, 2017. DOI: 10.1136/
vr.103948
- Åkerstedt J, Hofshagen M: Bacteriological investigation of infectious
keratoconjunctivitis in Norwegian Sheep. Acta Vet Scand, 45 (1): 19-26, 2004.
DOI: 10.1186/1751-0147-45-19
- Angelos JA: Overview of infectious keratoconjunctivitis. https://www.
msdvetmanual.com/eye-and-ear/infectious-keratoconjunctivitis/overviewof-infectious-keratoconjunctivitis;
Accessed: 25.10.2017.
- Radostits OM, Gay C, Hinchcliff KW, Constable PD: A textbook of the
diseases of cattle, horses, sheep, pigs and goats. Veterinary Medicine. 10th
ed., 1548-1551, London: Saunders, 2007.