The present study was performed to demonstrate the tissue differentiation of uterine ultrasonographic image by computer assisted analysis programs in mares with endometrial cyst and early pregnancy. A total of 124 thoroughbred Arabian mares were used in the study, with early pregnancy and endometrial cyst. Mares were divided into six groups according to stage of early pregnancy (Group I, n=12 (13th day pregnancy); Group II, n=15 (14th day pregnancy); Group III, n=29 (15th day pregnancy); Group IV, n=17 (16th day pregnancy); Group V, n=12 (17th day pregnancy) and as endometrial cysts (Group VI, n=39). The mean gray value (MGV), heterogeneity (HET) and contrast (CON) of the uterine images that were recorded during ultrasound scan were measured through computer assisted program. The MGV values of the echotexture parameters were significantly higher in the pregnant mare (55.20±4.95, 58.31±3.88, 56.23±1.88, 54.15±3.47 and 56.08±2.94, respectively) than the endometrial cyst mares (42.71±0.71) (P
Sunulan çalışma kısraklarda erken gebelik ile endometriyal kistli uterusun ultrasonografik görüntüsünün bilgisayar destekli analiz programları ile ayrımını ortaya koymak amacıyla yapıldı. Çalışmada erken gebe ve endometriyal kistli olmak üzere toplam 124 safkan Arap kısrak kullanıldı. Kısraklar erken gebelik dönemlerine (Grup I, n=12 (13 günlük gebelik); Grup II, n=15 (14 günlük gebelik); Grup III, n=29 (15 günlük gebelik); Grup IV, n=17 (16 günlük gebelik); Grup V, n=12 (17 günlük gebelik) ve endometriyal kist (Grup VI, n=39) olmak üzere altı gruba ayrıldı. Ultrasonografik muayene sırasında kaydedilen ve daha sonra bilgisayara aktarılan uterus görüntülerinin ortalama gri değeri (MGV), heterojenitesi (HET) ve kontrastı (CON) ölçüldü. Ekotekstür parametrelerinden MGV değeri gebe kısraklarda (sırasıyla; 55.20±4.95, 58.31±3.88, 56.23±1.88, 54.15±3.47 ve 56.08±2.94), endometrial kistli kısraklardan (42.71±0.71) daha yüksekti (P
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Arashiro EK, Fonseca JF, Siqueira LGB, Fernandes CA, Brandao FZ, Oba E, Viana JH: Assessment of luteal function in goats by ultrasonographic image attribute analysis. Small Ruminant Res, 94, 176-179, 2010. DOI: 10.1016/j. smallrumres.2010.07.007
Simoes J, Almeida JC, Baril G, Azevedo J, Fontes P, Mascarenhas R: Assessment of luteal function by ultrasonographic appearance and measurement of corpora lutea in goats. Anim Reprod Sci, 97, 36-46, 2007. DOI: 10.1016/j.anireprosci.2006.01.006
Ginther OJ: Tubular genitalia. In, Ginther OJ (Ed): Ultrasonic Imaging and Animal Reproduction. 105-128, Equiservices Publishing, Cross Plains, USA, 1998.
Griffin PG, Ginther OJ: Dynamics of uterine diameter and endometrial morphology during the estrous cycle and early pregnancy in mares. Anim Reprod Sci, 25, 133-142, 1991. DOI: 10.1016/0378-4320(91)90038-2
Bonafos LD, Kot K, Ginther OJ: Physical characteristics of the uterus during the bovine estrous cycle and early pregnancy. Theriogenology, 43, 713- 721, 1995. DOI: 10.1016/0093-691X(95)00014-Y
Siqueira LGB, Torres CAA, Amorim LS, Souza ED, Camargo LSA, Fernandes CAC, Viana JHM: Interrelationships among morphology, echotexture, and function of the bovine corpus luteum during the estrous cycle. Anim Reprod Sci, 115 (1-4): 18-28, 2009. DOI: 10.1016/j.anireprosci.2008.11.009
Tom JW, Pierson RA, Adams GP: Quantitative echotexture analysis of bovine corpora lutea. Theriogenology, 49, 1345-1352, 1998. DOI: 10.1016/ S0093-691X(98)00081-8
Pierson RA, Ginther OJ: Ultrasonic evaluation of the preovulatory follicle in the mare. Theriogenology, 24, 359-368, 1985. DOI: 10.1016/0093- 691X(85)90228-6
Bertmann J, Diaz L, Weber F, Stolla R, Bollwein H: Changes inuterine blood flow and endometrial echostructure during the earlypregnancy of cows. Wiener Tierärzt Monat, 91, 11, 2004.
Griffin RL, Bennett SD: Nd:YAG laser photoablation of endometrial cysts: A review of 55 cases (2000-2001). Proceedings AAEP, Orlando, Fla, 48, 58-60, 2002.
Ragon AC: Diagnosis and management of uterine cysts. Proceedings American College of Theriogenologists and Society for Theriogenology Mare Reproduction Symposium. August, Hastings, Neb, 21-26, 1996.
Adams GP, Kastelic JP, Bergfelt DR, Ginther OJ: Effect of uterine inflammation and ultrasonically-detected uterine pathology on fertility in the mare. J Reprod Fertil Suppl, 35, 445-454, 1987.
Wilson GL: Equine hysteroscopy: equipment for diagnostic endoscopy and photography. Vet Med, 80, 76-88, 1985.
Tannus RJ, Thun R: Influence of endometrial cysts on conception rate of mares. Zentralbl Veterinarmed A, 42, 275-283, 1995. DOI: 10.1111/j.1439- 0442.1995.tb00378.x
Bilkslager AT, Tate LP, Weinstock D: Effects of neodymium:yttrium aluminum garnet laser irradiation on endometrium and on endometrial cysts in six mares. Vet Surg, 22, 351-356, 1993. DOI: 10.1111/j.1532-950X.1993. tb00411.x
Bracher V, Mathias S, Allen WR: Videoendoscopic evaluation of the mare’s uterus: II. Findings in subfertile mares. Equine Vet J, 24, 279-284, 1992. DOI: 10.1111/j.2042-3306.1992.tb02835.x
Ginther OJ: The single embryo. Ultrasonic imaging and reproductive events in the mare. Cross Plains, Wis: Equiservices, 212-213, 1986.
Erdoğan G, Küçük N, Kanca H, Aksoy M: In vivo and in vitro assessment of ovarian echotexture through computer assisted real time ultrasonography in bitches. Ankara Üniv Vet Fak Derg, 64, 171-176, 2017.
McDowell KJ, Sharp DC, Grubaugh W, Thatcher WW, Wilcox CJ: Restricted conceptus mobility results in failure of pregnancy maintenance in mares. Biol Reprod, 39, 340-348, 1988. DOI: 10.1095/biolreprod39.2.340
Ley WB, Higbee RG, Holyoak GR: Laser ablation of endometrial and lymphatic cysts. Clin Tec Equine Pract, 1 (1): 28-31, 2002. DOI: 10.1053/ ctep.2002.33174
Kenney RM, Ganjam VK: Selected pathological changes of the mare uterus and ovary. J Reprod Fertil Suppl, 23, 335-339, 1975.
Cengiz M, Çolak A, Hayırlı A, Cannazik O: Optical density changes in ultrasonographic images of the endometrium and corpus luteum in pregnant and cyclic cows. Turk J Vet Anim Sci, 41, 18-24, 2017. DOI: 10.3906/vet-1604-41
Scully S, Evans ACO, Carter F, Duffy P, Lonergan P, Crowe MA: Ultrasound monitoring of blood flow and echotexture of the corpus luteum and uterus during early pregnancy of beef heifers. Theriogenology, 83, 449-458, 2015. DOI: 10.1016/j.theriogenology.2014.10.009
Polat B, Cengiz M, Cannazik O, Çolak A, Oruç E, Altun S, Salar S, Baştan A: Endometrial echotexture variables in postpartum cows with subclinical endometritis. Anim Reprod Sci, 155, 50-55, 2015. DOI: 10.1016/j. anireprosci.2015.01.015
Noseir WMB, Sosa GAM: Treatment of ovarian cysts in Buffaloes with emphasis to echotexture analysis. J Dairy Vet Anim Res, 2 (2): 1-7, 2015. DOI: 10.15406/jdvar.2015.02.00030
Herzog K, Kiossis E, Bollwein H: Examination of cyclic changes in bovine luteal echotexture using computer-assisted statistical pattern recognition techniques. Anim Reprod Sci, 106, 289-297, 2008. DOI: 10.1016/j. anireprosci.2007.05.004
Cengiz M, Kanca H, Salar S, Bastan A, Kucukaslan I, Alkan H, Karakas K, Yuksel O, Hayirli A: Endometrial echotexture parameters in Turkish Saanen Goats (Akkeci) during oestrus and early pregnancy. Anim Reprod Sci, 146, 27- 33, 2014. DOI: 10.1016/j.anireprosci.2014.02.004
Kucukaslan I: Evaluation of endometrial echostructure following Lotagen® administiration in cows with chronic endometritis. PhD Thesis, Institute of Health Sciences, Ankara University, 2010.
Kauffold J, Von Dem Bussche B, Failing K, Wehrend A, Wendt M: Use of B-mode ultrasound and grey-scale analysis to study uterine echogenicity in the pig. J Reprod Dev, 56, 444-448, 2010. DOI: 10.1262/jrd.09-220T
Schmauder S, Weber F, Kiossis E, Bollwein H: Cyclic changes in endometrial echotexture of cows using a computer-assisted program for the analysis of first-and second-order grey level statistics of B Mode ultrasound images. Anim Reprod Sci, 106, 153-161, 2008. DOI: 10.1016/j.anireprosci.2007.12.022
Ginther OJ: How ultrasound technologies have expanded and revolutionized research in reproduction in large animals. Theriogenology, 81, 112-125, 2014. DOI: 10.1016/j.theriogenology.2013.09.007
Liu X, Dai Q, Hart EJ, Barrett DMW, Rawlings NC, Pierson RA, Bartlewski PM: Ultrasonographic characteristics of ovulatory follicles and associated endocrine changes in cyclic ewes treated with medroxyprogesterone acetate (MAP)-releasing intravaginal spongesand equine chorionic gonadotropin (eCG). Reprod Domest Anim, 42, 393-401, 2007. DOI: 10.1111/j.1439-0531.2006.00798.x
Davies KL, Bartlewski PM, Pierson RA, Rawlings NC: Computer assisted image analyses of corpora lutea in relation to peripheral concentrations of progesterone: A comparison between breeds ofsheep with different ovulation rates. Anim Reprod Sci, 96, 165-175, 2006. DOI: 10.1016/j.anireprosci. 2005.12.003
Vassena R, Adams GP, Mapletoft RJ, Pierson RA, Singh J: Ultrasound image characteristics of ovarian follicles in relation to oocyte competence and follicular status in cattle. Anim Reprod Sci, 76, 25-41, 2003. DOI: 10.1016/S0378- 4320(02)00234-8
Gabor G, Sasser RG, Kastelic JP, Mezes M, Falkay G, Bozo S, Csik JV, Barany I, Hidas A, Jr Szasz F, Boros G: Computer analysis of video and ultrasonographic images for evaluation of bull testes. Theriogenology, 50, 223- 228, 1998. DOI: 10.1016/S0093-691X(98)00129-0
Singh J, Adams GP, Pierson RA: Promise of new imaging technologies for assessing ovarian function. Anim Reprod Sci, 78, 371-399, 2003. DOI: 10.1016/ S0378-4320(03)00100-3
Griffin PG, Ginther OJ: Research application of ultrasonic imaging in reproductive biology. J Anim Sci, 70, 953-972, 1992. DOI: 10.2527/1992.703953x
Zagzebski JA: Physics of diagnostic ultrasound. In, Zagzebski JA (Ed): Essentials of Ultrasound Physics. 1-19, Mosby Year Book Inc, St. Louis, 1996.
Singh J, Pierson RA, Adams GP: Ultrasound image attributes of the bovine corpus luteum: structural and functional correlates. J Reprod Fertil, 109, 35-44, 1997. DOI: 10.1530/jrf.0.1090035
Pierson RA, Adams GP: Computer-assisted image analysis, diagnostic ultrasonography and ovulation induction: Strange bedfellows. Theriogenology, 43, 105-112, 1995. DOI: 10.1016/0093-691X(94)00014-L
Pierson RA, Ginther OJ: Ultrasonographic appearance of the bovine uterus during the estrous cycle. J Am Vet Med Assoc, 190, 995-1001, 1987.
Fissore RA, Edmondson AJ, Pashen RL, Bondurant RH: The use of ultrasonography for the study of the bovine reproductive tract. II. Nonpregnant, pregnant and pathological conditions of the uterus. Anim Reprod Sci, 12, 167-177, 1986. DOI: 10.1016/0378-4320(86)90037-0
Reeves JJ, Rantanen NW, Hauser M: Transrectal real-time ultrasound scanning of the cow reproductive tract. Theriogenology, 21, 485-494, 1984. DOI: 10.1016/0093-691X(84)90410-2
Pierson RA, Ginther OJ: Ultrasonography of the bovine ovary. Theriogenology, 21, 495-504, 1984. DOI: 10.1016/0093-691X(84)90411-4
Palmer E, Driancourt MA: Use of ultrasonic echography in equine gynecology. Theriogenology, 13, 203-216, 1980. DOI: 10.1016/0093-691X (80)90082-5