Osman ERGENE, Isfendiyar DARBAZ, Serkan SAYINER, Selim ASLAN

Comparison of Bovine Pregnancy Associated Glycoproteins (bPAGSerum and Milk), Bovine Pregnancy Specific Protein B (bPSP-B) Tests with Each Other and with Transrectal Ultrasonographic Findings for Early Pregnancy Diagnosis

The aim of this study was to compare the accuracy of bovine pregnancy-associated glycoproteins (bPAG) and bovine pregnancy-specific protein B (bPSP-B) tests with transrectal ultrasonography (TRUS) for early pregnancy diagnosis in dairy cows. The study was carried out on 58 Holstein cows. Blood and milk samples were collected on days 20, 25, 28, 30, 32 and 40 following artificial insemination (AI). TRUS was performed on all cows on days 30 and 40 following AI. The positive predictive values for bPAG-serum and bPAG-milk tests were 100% on days 28 and 30. For bPSP-B tests, positive predictive values were 93.7% and 100% on days 28 and 30, respectively. The Kappa test revealed a high agreement between TRUS and the three tests on days 28 and 30 with the values of 0.954 and 0.946 (P

Erken Gebelik Teşhisi Amacıyla Sığır Gebelik İlişkili Glikoprotein (bPAGSerum ve Süt) ve Sığır Gebelik Spesifik Protein B (bPSP-B) Testlerinin Birbirileriyle ve Transrektal Ultrasonografik Bulgularla Karşılaştırılması

Bu çalışmanın amacı, süt ineklerinin erken gebelik tanısında sığır gebelik ilişkili glikoproteinlerin (bPAG) ve sığır gebelik spesifik protein B (bPSP-B) testlerinin transrektal ultrasonografiyle (TRUS) doğruluğunun karşılaştırılmasıdır. Araştırma 58 adet Holstein ineğinde gerçekleştirildi. Suni tohumlamayı (AI) takiben 20, 25, 28, 30, 32 ve 40. günlerde kan ve süt örnekleri toplandı. TRUS, AI’yi takiben 30. ve 40. günlerde tüm ineklerde yapıldı. bPAG-serum ve bPAG-süt testleri için pozitif prediktif değerler, 28 ve 30. Günlerde %100 idi. bPSP-B testleri için, pozitif prediktif değerler sırasıyla 28 ve 30. Günlerde %93.7 ve %100 idi. Kappa testi TRUS ile 28 ve 30. günlerde yapılan üç test arasında sırasıyla 0.954 ve 0.946 (P

PDF

___

Howard J, Gabor G, Gray T, Passavant C, Ahmadzadeh A, Sasser N, Pals D, Sasser G: BioPRYN, a blood-based pregnancy test for managing breeding and pregnancy in cattle. Proc West Sect Am Soc Anim Sci,58, 295-298, 2007.

Green JA, Parks TE, Avalle MP, Telugu BP, McLain AL, Peterson AJ, McMillan W, Mathialagan N, Hook RR, Xie S, Roberts RM: The establishment of an ELISA for the detection of pregnancy-associated glycoproteins (PAGs) in the serum of pregnant cows and heifers. Theriogenology, 63 (5): 1481-1503, 2005. DOI: 10.1016/j.theriogenology. 2004.07.011

Zoli AP, Guilbault LA, Delahaut P, Ortiz WB, Beckers JF: Radioimmunoassay of a bovine pregnancy-associated glycoprotein in serum - Its application for pregnancy diagnosis. Biol Reprod, 46 (1): 83-92, 1992. DOI: 10.1095/biolreprod46.1.83

Breed MW, Guard CL, White ME, Smith MC, Warnick LD:Comparison of pregnancy diagnosis in dairy cattle by use of a commercial ELISA and palpation per rectum. J Am Vet Med Assoc, 235 (3): 292-298, 2009. DOI: 10.2460/javma.235.3.292

Gáspárdy A, Schwartz Z, Zöldág L, Veresegyházy T, Fekete S: Changes in daily energy amounts of main milk components (lactose, protein and fat) during the lactation of high-yielding dairy cows. Acta Vet Hung, 52 (4): 457-467, 2004. DOI: 10.1556/AVet.52.2004.4.8

Friedrich M, Kalscheuer E, Hoedemaker M, Holtz W: Pregnancy detection in dairy cows by measuring pregnancy-associated glycoprotein (PAG) in milk. Reprod Domest Anim, 42, 70, 2007.

López-Gatius F, Garbayo JM, Santolaria P, Yániz J, Ayad A, de Sousa NM, Beckers JF: Milk production correlates negatively with plasma levels of pregnancy-associated glycoprotein (PAG) during the early fetal period in high producing dairy cows with live fetuses. Domest Anim Endocrinol, 32 (1): 29-42, 2007. DOI: 10.1016/j.domaniend.2005.12.007

Le Roux Y, Colin O, Laurent F: Proteolysis in samples of quarter milk with varying somatic cell counts. 1. Comparison of some ındicators of endogenous proteolysis in milk. J Dairy Sci, 78, 1289-1297, 1995. DOI: 10.3168/jds.S0022-0302(95)76749-2

Silva E, Sterry RA, Kolb D, Mathialagan N, McGrath MF, Ballam JM, Fricke PM: Accuracy of a pregnancy-associated glycoprotein elısa to determine pregnancy status of lactating dairy cows twenty-seven days after timed artificial ınsemination. J Dairy Sci, 90 (10): 4612-4622, 2007. DOI: 10.3168/jds.2007-0276

Romano JE, Thompson JA, Kraemer DC, Westhusin ME, Forrest DW, Tomaszweski MA: Early pregnancy diagnosis by palpation per rectum: Influence on embryo/fetal viability in dairy cattle. Theriogenology, 67 (3): 486-493, 2007. DOI: 10.1016/j.theriogenology.2006.08.011

Friedrich M, Holtz W: Establishment of an ELISA for measuring bovine pregnancy-associated glycoprotein in serum or milk and its application for early pregnancy detection. Reprod Domest Anim, 45 (1): 142-146, 2010. DOI: 10.1111/j.1439-0531.2008.01287.x

Green JC, Volkmann DH, Poock SE, McGrath MF, Ehrhardt M, Moseley AE, Lucy MC: Technical note: A rapid enzyme-linked immunosorbent assay blood test for pregnancy in dairy and beef cattle. J Dairy Sci, 92 (8): 3819-3824, 2009. DOI: 10.3168/jds.2009-2120

Buragohain L, Nanda T, Ghosh A, Ghosh M, Kumar R, Kumar S, Gupta SS, Bharali A, Mohanty AK, Singh I, Balhara AK: Identification of serum protein markers for early diagnosis of pregnancy in buffalo. Anim Sci J, 88 (8): 1189-1197, 2017. DOI: 10.1111/asj.12754

Romano JE, Larson JE: Accuracy of pregnancy specific protein-B test for early pregnancy diagnosis in dairy cattle. Theriogenology, 74 (6): 932- 939, 2010. DOI: 10.1016/j.theriogenology.2010.04.018

Szenci O, Beckers JF, Humblot P, Sulon J, Sasser G, Taverne MAM, Varga J, Baltusen R, Schekk G: Comparison of ultrasonography, bovine pregnancy-specific protein B and bovine pregnancy-associated glycoprotein 1 tests for pregnancy detection in dairy cows. Theriogenology, 50 (1): 77-88, 1998. DOI: 10.1016/S0093-691X(98)00115-0

Quintela LA, Barrio M, Peña AI, Becerra JJ, Cainzos J, Herradón PG, Díaz C: Use of ultrasound in the reproductive management of dairy cattle. Reprod Domest Anim, 47 (Suppl 3): 34-44, 2012. DOI: 10.1111/j.1439- 0531.2012.02032.x

Holman A, Thompson J, Routly JE, Cameron J, Jones DN, GroveWhite D, Smith RF, Dobson H: Comparison of oestrus detection methods in dairy cattle. Vet Rec, 169 (2): 47, 2011. DOI: 10.1136/vr.d2344

Walsh SW, Williams EJ, Evans ACO: A review of the causes of poor fertility in high milk producing dairy cows. Anim Reprod Sci, 123 (3-4): 127- 138, 2011. DOI: 10.1016/j.anireprosci.2010.12.001