ALİYE GÜLMEZ SAĞLAM, EKİN EMRE ERKILIÇ, Fatih BÜYÜK, ALİ HAYDAR KIRMIZIGÜL, Gürbüz GÖKÇE, LOKMAN BALYEN, ENES AKYÜZ, Uğur AYDIN, BURHAN ÖZBA, Salih OTLU

Moraxella ovis and Mycoplasma conjunctivae Isolation from an Ovine Infectious Keratoconjunctivitis Outbreak and Fortified Treatment Approaches

The aim of this study was to determine the etiology of naturally occurring infectious keratoconjunctivitis (IKC) outbreak observed in a Tuj sheep herd rearing semi-intensively and to try fortified treatment options in symptomatic sheeps. Conjunctival samples from 42 sheep with keratoconjunctivitis were collected aseptically using sterile cotton swabs. All swab were cultivated for bacterial isolation. To test treatment options symptomatic animals were randomly divided into 3 groups each were composed of adult ewes. The first group (n=13) was treated with the subconjunctival administration of penicillin G potassium (250.000 IU/animal), the second group (n=14) was treated with oxytetracycline and polymyxin B sulfate, and the third group (n=15) was treated with amikacin sulfate (diluted with isotonic NaCl to 25 mg per mL), ceftriaxone disodium and fluconazole (2 mg/mL) combination. As the results of the bacteriological examination, 36 (85.71%) of 42 conjunctival samples were found positive for Moraxella ovis. Also, Mycoplasma conjunctivae was identified in 3 (7.14%) conjunctival samples positive for M. ovis. After treatment, 84.61% (11/13) of the first group; 71.42% (10/14) of the second group and all animals in the third group were observed to be healed. This study demonstrated that M. ovis was the primary pathogen causing IKC in Tuj sheeps and M. conjunctivae was the second rank. The combination of amikacin sulfate, ceftriaxone disodium and fluconazole have been identified as the most effective treatment option of IKC in sheep.

Bir İnfeksiyöz Koyun Keratokonjonktivit Salgınından Moraxella ovis ve Mycoplasma conjunctivae İzolasyonu ve Güçlendirilmiş Tedavi Yaklaşımı

Bu çalışmada, semi-entansif olarak yetiştirilen bir Tuj koyun sürüsünde doğal olarak gözlenen bir infeksiyöz keratokonjonktivit (IKC) salgınının etyolojisinin belirlenmesi ve semptom gösteren koyunlarda kuvvetlendirilmiş tedavi seçeneklerinin denenmesi amaçlanmıştır. Klinik keratokonjunktivitis belirtisi olan 42 koyunun konjunktival örnekleri, steril sıvap kullanılarak aseptik koşullarda toplandı. Tüm örnekler bakteri izolasyonu için kültüre edildi. Tedavi seçeneklerini test etmek için ergin koyunlardan oluşan semptomatik hayvanlar rastgele 3 gruba ayrıldı. Birinci grupta (n=13) penisilin G potasyum’un (250.000 IU/hayvan) subkonjunktival uygulanarak, ikinci grupta (n=14) oksitetrasiklin ve polimiksin B sülfat’ın gözkapağı içine mercimek tanesi büyüklüğünde konularak ve üçüncü grupta (n=15) ise amikasin sülfat, seftriakson disodyum (0.5 g) ve flukonazol (2 mg/mL) kombinasyonu damlatma şeklinde uygulanarak tedavi denemeleri yapıldı. Bakteriyolojik muayene sonucunda, 42 konjunktival sıvap örneğinin 36’sında (%85.71) Moraxella ovis ve bu pozitif örneklerin 3’ünde (%7.14) Mycoplasma conjunctivae izole edildi. Tedavi sonrası, birinci grubun %84.61’sı (11/13); ikinci grubun %71.42’si (10/14) ve üçüncü grubun ise tümünde iyileşme görüldü. Bu çalışma, M. ovis’in Tuj koyunlarında IKC’ye neden olan primer patojen, M. conjunctivae’nın ise sekonder etken olduğunu göstermiştir. Amikasin sülfat, seftriakson disodyum ve flukonazol kombinasyonu, koyunlarda IKC’nin en etkili tedavi seçeneği olarak tanımlanmıştır.

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