ŞİMA ŞAHİNDURAN, ÖZLEM ÖZMEN, REYDA KIYICI

Determination of Telomerase Enzyme Level in Enzootic Intranasal Tumors of Goats

Enzootic intranasal adenocarcinoma (ENA) of goats are contagious tumoral disease that characterized occurrence of tumoral masses in nasal cavity of the animals. The etiological agent of the disease is retrovirus and the disease cause high economical loses in goat industry. Retroviruses, respectively inducing lung or nasal adenocarcinomas. The true economic impact of ENA is not known because affected animals are given insights from the herd before actual diagnosis, and suspected disease incidence is rarely reported. Telomerase regulates the proliferative capacity of cells and this enzyme has critical role in tumor progression. The aim of this study is to evaluate telomerase activity in serum and tumoral tissue in goat with ENA. For this aim 11 goat’s serum were analyses for telomerase activity. Tumoral tissue sections of totally 14 death goats were examined by immunohistochemically for telomerase expression. In addition, 10 normal goat’s serum and nasal tissues that belong slaughtered healthy animals used as control. This study showed that increased serum and tissue telomerase activity in goats with ENA comparing to controls. Also our results showed that telomerase activity becomes a useful prediction marker for tumor development in infected but clinically healthy goats in infected flocks

Keçilerin Enzootik Intranazal Tümörlerinde Telomeraz Enzim Düzeyinin Belirlenmesi

Keçilerin enzootik intranazal adenokarsinomu (ENA), hayvanların burun boşluğunda tümoral kitlelerin şekillenmesi ile karakterize bulaşıcı bir hastalıktır. Hastalığın etkeni retrovirüs olup, keçi endüstrisinde ekonomik kayıplara neden olmaktadır. Retroviruslar sırasıyla akciğer veya nazal adenokarsinomlara yol açar. Hastalıktaki ekonomik kayıp tam olarak bilinmemektedir. Çoğu zaman hasta hayvanlar teşhisten önce sürüden çıkarıldıkları için hastalığın gerçek insidansı ile ilgili bildirimler nadirdir. Telomeraz, hücrelerin proliferatif kapasitesini düzenler ve bu enzim tümörün yayılmasında kritik bir role sahiptir. Bu çalışmanın amacı ENA’lı keçilerin kan serumu ve tümöral dokularında telomeraz aktivitesini değerlendirmektir. Bu amaçla, 11 ENA’lı keçi serumunda telomeraz aktivitesi için analizler yapıldı. Ayrıca toplam 14 ölü keçinin tümoral doku kesitleri, telomeraz ekspresyonu için immunohistokimyasal olarak incelendi. Kontrol olarak, kesimi yapılan 10 sağlıklı keçinin serumu ve burun dokusu kullanıldı. Bu çalışmada ENA’lı keçilerde serum ve tümör dokularında telomeraz aktivitesinin kontrol grubundaki keçilere kıyasla arttığı tespit edildi. Enfekte olmuş fakat klinik olarak sağlıklı görünen keçilerde telomeraz aktivitesinin tümör gelişimi için yararlı bir belirteç olabileceği görüldü.

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