MAHMUT OK, CENK ER, RAMAZAN YILDIZ, Ramazan ÇÖL, UĞUR AYDOĞDU, İSMAİL ŞEN, HASAN GÜZELBEKTEŞ

Evaluation of Acute Phase Proteins, Some Cytokines and Hemostatic Parameters in Dogs with Sepsis

Bu çalışmanın amacı; sepsisli köpeklerde akut faz proteinler, bazı sitokinler ve hemostatik sistem parametrelerin değişimlerini değerlendirerek, hastalığın tanısında bu parametrelerin önemini ortaya koymaktır. Bu çalışmanın materyalini 30 sepsisli ve 9 sağlıklı köpek oluşturdu. Sepsisli köpeklerde iştahsızlık, durgunluk, depresyon, vücut ısısında artış, mukoz membranlarda konjesyon, kapiller dolum zamanında uzama, taşikardi, takipnea, lökositozis veya lökopeni belirlendi. Bütün köpeklerin interlökin-1ß (IL-1ß), tümör nekroz faktör α (TNF α), interferon γ (INF- γ), C-reaktif protein (CRP), serum amiloid A (SAA) ve protrombin zamanı (PT), aktive edilmiş parsiyel tromboplastin zamanı (aPTT), antitrombin III (AT III), fibrinojen, protein C ve D-dimer seviyeleri ölçüldü. Sepsisli köpeklerde serum IL-1ß, TNF-α, INF-γ, CRP ve SAA düzeylerinde önemli artış belirlendi. Sepsisli köpeklerde plazma PT ve APTT sürelerinde önemli uzama, fibrinojen, D-dimer ve protein C düzeylerinde önemli artış, AT-III düzeyinde ise önemli azalma tespit edildi. Sonuç olarak sepsisli köpeklerde SAA, IL-1ß ve TNFα paramterelerinin yangısel olaylarda önemli rol aldığı belirlendi. Sepsisli köpeklerde tespit edilen hemostatik anormallikler dissemine intravaskuler koagulasyon (DİK) gelişimi ile ilgili olabilir.

Sepsisli Köpeklerde Akut Faz Proteinler, Bazı Sitokinler ve Hemostatik Parametrelerin Değerlendirilmesi

The aim of this study was to evaluate the alterations in acute phase proteins, cytokines and hemostatic parameters in dogs with sepsis and to determine the importance of these parameters in diagnosis of the sepsis. Thirty dogs with sepsis and 9 healthy dogs were used in this study. Anorexia, depression, lethargy, hyperthermia, tachycardia, tachypnea, congestion in the mucosal membranes, prolonged capillary refill time, and leukocytosis or leucopenia were identified in the dogs with sepsis. The serum interleukin-1ß (IL-1ß), tumor necrosis factor α (TNF-α), interferon γ (INF-γ), C-reactive protein (CRP), serum amyloid A (SAA), prothrombin time (PT), activated partial thromboplastin time (aPTT), antithrombin III (AT III), fibrinogen, protein C (PC), and D-dimer levels were measured in all dogs. We found that the serum IL-1ß, TNF-α, INF-γ, CRP and SAA concentrations were significantly elevated in dogs with sepsis as compared with healthy controls. In addition, the plasma PT and APTT levels were notably prolonged, the plasma fibrinogen, D-dimers and protein C concentrations were significantly increased. However, the antithrombin III activity was significantly decreased in the dogs with sepsis. In conclusion, the results of this study indicate that the SAA, IL-1ß and TNF-α parameters play important roles in the inflammatory process in dogs with sepsis. The hemostatic abnormalities observed in dogs with sepsis may be due to the development of disseminated intravascular coagulation (DIC).

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