Fereshteh KHOMEJANI FARAHANI, Hamidreza FATTAHIAN, Abdol-Mohammad KAJBAFZADE

Experimental Study on Ostrich Acellular Dermal Matrix in Repair of Full-Thickness Wounds of Guinea Pig

ADM (Asellüler dermal matriks) tüm katmanları kapsayan yaralarda standart bir tedavi şekli olabilir. Bu çalışmanın amacı biyomedikal bir uygulama olarak devekuşu desellüler matriksin oluşturulması ve yara iyileşmesinde ADM uygulamasının başarısının değerlendirilmesidir. On sekiz (n=18) kobay çalışmada kullanıldı ve bu hayvanların yan taraflarında 2X2 cm boyutlarında deri yaraları oluşturuldu. Deneme grubu olarak 9 hayvana asellüler devekuşu dermal matriksi uygulanırken diğer 9 hayvan kontrol grubu olarak kullanıldı ve normal devekuşu derisi uygulandı. Epitelizasyon ve diğer histoloj ik bulgular 3., 7. ve 26. günlerde değerlendirildi (Kruskal-wallis ve Mann Whitney U testi) ve operasyondan 26 gün sonra yara durumuna bakıldı (ANOVA ve Tukey testi). Her iki uygulama yara kontraktürünü azaltırken çalışma sonunda anlamlı bir fark tespit edilmedi (P˃0.05). Gruplar arasında fibroblast infiltrasyonu ve kolajen çatı altında damarlaşma açısından bir fark belirlenmedi (P=0.06). ADM uygulanan grupta yara epitelizasyonunun ve neodermisin histoloj ik değerlendirmesinde çalışma süreci sonunda daha kalın neodermis oluşumu gözlemlendi. Çalışma sonunda elde edilen bulgular yara iyileşmesinde asellüler dermal matriksin geçici olarak kullanılabileceğini göstermektedir.

Kobaylarda Tüm-Deri Katmanlarını İçeren Yaralarda Devekuşu Asellüler Dermal Matriksin Etkisinin Araştırıldığı Deneysel Bir Çalışma

ADM (Acellular dermal matrix) can be a standard treatment for full-thickness wound defects. The aim of the present study was to achieve a decellularized matrix of the ostrich as a biomedical application and assess the evaluation of wound healing, which were implanted with ADM. Eighteen (n=18) guinea pigs were conducted and they received a full- thickness 2×2 cm flank skin wounds, nine experiment were implanted with acellular ostrich dermal matrix as experimental group and nine experiment as control group, which implanted with normal ostrich skin. At days of 3, 7 and 26 were evaluated for epithelialization and the other histological characteristics (Kruskal-wallis and Mann Whitney U tests), and the wounds were assessed for wound contracture at 26 days after surgery (ANOVA and Tukey test). Each dermal substitute decreased wound contracture and show no significant different at the endpoint of study (P˃0.05). Between groups, increasing infiltration of fibroblast and new blood vessels under collagen scaffold showed no significant different (P=0.06). Histological evaluation of wound epithelialization and neo-dermis which was produced by ADM, was thicker neo-dermis at the end of study in experimental group. The study revealed that ostrich acellular dermal matrix can be used as a temporary substitute with acceptable result in healing of full-thickness wounds.

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