Gülşen ÇIĞŞAR, Osman Nuri KELEŞ, Serpil CAN, Mehmet KARACA, İSMAİL CAN, HÜSEYİN SERKAN EROL, Dilek SAĞIR, Özlem Özgül ABUÇ, AHMET ÇAKIR, Zişan Gönül ŞAHİN, Bünyami ÜNAL, BARIŞ ANIL, MESUT BÜNYAMİ HALICI

The Protective Effects of Osajin on Ischemia/Reperfusion Injury to Rat Ovaries: Biochemical and Histopathological Evaluation

İnflamasyon ve Reaktif Oksijen Sınıfları (ROS) iskemi reperfüzyon (I/R) over hasarlarında çok önemli rol oynar. Bu in vivo çalışmanın amacı tek taraflı I/R ile oluşturulan over hasarı ve antiinflamatuvar ve antioksidant özellikli bir flavonoid olan osaj inin oksidatif denge üzerine etkilerini incelemektir. Çalışmada 48 yetişkin dişi Wistar albino rat kullanıldı. Kontrol (CN) grubuna yalnızca laparatomi uygulandı. CNOsaj in grubunda, sadece 200 mg/kg dozda osaj in verildi. IRVEHICLE grubunda, 3 saatlik iskemik periyodu 3 saatlik reperfüzyon takip etti; daha sonra bilateral yumurtalıklar alındı. IROsaj in100 ve IROsaj in200 gruplarında 3 saatlik iskemiden sonra sırasıyla 100 ve 200 mg/kg osaj in reperfüzyondan önce oral olarak verildi.; 3 saatlik reperfüzyondan sonra yumurtalıklar alındı. Deneylerden sonra, PMNlerin aktiviteleri ve oksidatif durumları için MPO, SOD ve CAT enzim aktiviteleri ve LPO düzeyleri belirlendi. Ek olarak histopatoloj ik değişimler tüm rat ovaryum dokularında incelendi. İstatistiksel analizler one-way ANOVA kullanılarak yapıldı (Duncan ile). Biyokimyasal ve histopatoloj ik sonuçlara göre, yüksek antioksidan SOD ve CAT enzim aktivitesine rağmen, I/R PMNlerin infiltrasyonunu, MPO aktivitelerini ve LPO düzeylerini artırdı. İskemik reperfüzyondan önce, osaj inin her doz seviyesi büyük bir düşüşe sebep olan yüksek dozla birlikte IRVEHICLE grubunda olanlarla karşılaştırıldığında LPO düzeyi, MPO aktivitesi ve PMN infiltrasyonunu önemli derecede düşürdü. Ek olarak sonuçlar dönüşümsüz ovaryum hasarının iyileştirmeden gelişmesine karşın osaj in ile tedavi I/R tarafından uyarılmış olduğunu gösterdi. Bu sonuçlar osaj inin ovariyan iskemik reperfüzyon hasarına karşı koruma sağladığını düşündürmektedir. Bunun mekanizmaları oksidatif stresi ve aktivitelerini ve PMN infiltrasyonunu azaltması ile ilgili olabilir.

Sıçan Ovaryumlarında İskemi/Reperfüzyon Hasarı Üzerine Osajinin Koruyucu Etkileri: Biyokimyasal ve Histopatolojik Değerlendirme

Reactive oxygen species (ROS) and inflammation play important roles in the pathogenesis of ischemia/reperfusion (I/R) ovarian inj ury. The purpose of this in-vivo study is to evaluate the effect of osaj in, a prenylated flavonoid with antioxidant and anti-inflammatory properties, on oxidative balance and ovarian damage induced by unilateral I/R. The study used 48 adult, female Wistar albino rats. In the controls (CN), only laparotomy was performed. In group CNOsaj in, 200 mg/kg osaj in was administered. In group IRVEHICLE, an ischemic period of 3 h was followed by reperfusion for 3 h; the bilateral ovaries were then removed. In groups IROsaj in100 and IROsaj in200, after 3 h of ischemia, 100 and 200 mg/kg of osaj in was given orally before reperfusion, respectively; after 3 h of reperfusion, the ovaries were removed. After the experiments, MPO, SOD and CAT enzyme activities and LPO levels was determined for the oxidative state and activities of PMNs. In addition, histopathological changes were examined in all rat ovarian tissues. Statistical analysis was performed using one-way ANOVA (with Duncan). According to biochemical and histopathological results, I/R increased LPO levels and MPO activities and infiltration of PMNs despite high-antioxidant SOD and CAT enzyme activity. Both dosage levels of osaj in before I/R significantly decreased LPO level and MPO activity and PMN infiltration compared to those of the IRVEHICLE group, with the higher dosage causing greater decreases. In addition, results showed that treatment with osaj in against ameliorated development of irreversible ovarian damage induced by I/R. These results suggest that osaj in provides protections against ovarian I/R inj ury. Its mechanisms could be related to mitigation of oxidative stress and activities and to PMN infiltration.

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