Kübra Asena KAPAKİN TERİM, Halit IMIK, RECEP GÜMÜŞ, Samet KAPAKIN, YAVUZ SELİM SAĞLAM

Effect of Vit E on secretion of HSP-70 in testes of broilers exposed to heat stress

Bu çalışmanın amacı broilerlerin testislerinde ısı stresinin sebep olduğu doku hasarı üzerine vitamin E (Vit E)’in iyileştirici etkisini araştırmak ve bu etkinin HSP-70 proteininin sekresyonu ile ilişkisini ortaya koymaktır. Çalışmada toplam olarak 30 adet (Ross 308 ırkı) broiler kullanıldı. Hayvanlar kontrol grubu da dahil olmak üzere üç gruba ayrıldı. Grup kontrol (C); sıcaklık stresine maruz bırakılmayan (24oC) ve diyetlerine Vit E eklenmeyen broiler. Grup stress (S); diyetlerine Vit E eklenmeyen ancak sıcaklık stresine maruz bırakılan (34oC) broiler. Grup Stres +Vitamin E (Grup S + vit E); sıcaklık stresine maruz bırakılan (34oC) ve diyetlerine vitamin E eklenen broiler. Histopatolojik inceleme S grubunun testislerinde dejeneratif değişiklikler gösterdi. Ancak S + Vit E grubunda dejenerasyonun şiddetinin azaldığı gözlendi. Grupların tamamının testislerinde farklı seviyede HSP-70 sekresyonu saptanırken, HSP-70’in en yüksek seviyesi grup C’de en düşük seviyesi grup S’deydi. Isı stresinin broilerlerin testislerinde HSP-70 sekresyonunu azaltığı, vitamin E uygulandığında, HSP-70 sekresyonunun yeniden artığı ve doku hasarını azalttığı sonucuna varıldı.

Anahtar Kelimeler:

yem zenginleştiriciler, ısı şoku proteinleri, etlik piliç, sıcaklık stresi, histopatoloji, kümes hayvanları, stres, stres yanıtı, testis, doku iyileşmesi, E vitamini, dejenerasyon, diyetler

Sıcaklık stresine maruz bırakılan broilerlerin testislerinde Vit E’ in HSP-70 sekresyonu üzerine etkisi

The objective of this experiment was to investigate the ameliorative effect of vitamin E (Vit E) on tissue damage caused by heat stress in the testes of broilers, and to correlate this effect with the secretion of the HSP-70 protein. In the study totally 30 broilers (breed Ross 308) were used. Animals were divided into three groups, including the control group. Group Control (C); these broilers (24oC) were not administered with Vit E in the diet and not subjected to heat stress. Group Stress (S); these broilers not administered with Vit E in the diet but subjected to heat stress (34oC). Group Stress +Vitamin E (Group S + vit E); broilers subjected to heat stress (34oC) and administered with vitamin E. Histopathological examination demonstrated the presence of degenerative alterations in testes tissue of the group S. However, there has been seen diminishing the severity of degeneration in the group S+Vit E. While secretion of HSP-70 was determined in the testes of all groups in varying degree, the highest secretion level of HSP-70 was in the group C, the lowest secretion level of HSP-70 was in the group S. It is concluded that heat stress reduces the secretion of HSP-70 in testes of broilers, when vitamin E is administered, the secretion of HSP-70 increases again and reduces tissues damage.

Keywords:

feed supplements, heat shock proteins, broilers, heat stress, histopathology, poultry, stress, stress response, testes, tissue repair, vitamin E, degeneration, diets,

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