AHMET KOLUMAN, Teoman UNLU, Abdullah DİKİCİ, Atanur TEZEL, E. Nazan AKCELIK, Zeynep T. BURKAN

Presence of Staphylococcus aureus and Staphylococcal Enterotoxins in Diff erent Foods

Staphylococcal enterotoksinler tüm dünyada gıda intoksikasyonlarında öncü etmenler arasında yer almaktadır. Bu çalışma üç aşamada gerçekleştirilmiştir. İlk aşamasında 300 faklı gıda örneğinde Staphylococcus aureus varlığı iki faklı agarda incelenmiş, takip eden aşamada aynı gıdalarda enterotoksinlerin varlığı araştırılmıştır. Son olarak elde edilen kültürlerin antibiyotik dirençlilikleri ölçülmüş ve Metsisiline dirençli S. aureus (MRSA) varlığı mecA PCR ile aranmıştır. Toplam 221 adet S. aureus suşu elde edilmiştir. İlgili suşlar antibiyotik direnci yönünden incelendiğinde her suşun en az iki antibiyotiğe dirençli olduğunu ancak MRSA'nın incelediğimiz örneklerde bulunmadığı ortaya konulmuştur. Örneklerde enterotoksinler için VIDAS ile tarama yapılıp ELISA ile toksinler tiplendirilmiştir. Vidas SET2 ile yapılan enterotoksin analizinde 300 örneğin 72sinin (%24) SE içerdiği ve ELISA ile bu enterotoksinlerin dağılımının farklı olduğu saptanmıştır.

Farklı Gıdalarda Staphylococcus aureus ve Stafilokokal Enterotoksinlerin Varlığı

Staphylococcal food poisoning is one of the most prevalent causes of gastroenteritis throughout the world. This study has three steps. In the first step of the study, diff erent food matrices were analyzed for the presence of Staphylococcus aureus. In the second step, types of staphylococcal enterotoxins (SET) from these foods were analyzed. Lastly, antibiotic resistance of S. aureus isolated was determined using conventional and PCR methods. For this purpose, 300 diff erent food samples were evaluated for contamination with S. aureus. From these samples 221 S. aureus strains were isolated. The same strains were evaluated for antibiotic resistance and existence of mecA gene. All strains were at least resistant to 2 antibiotics, but no MRSA strain was detected. Staphylococcal Enterotoxins (SE) were determined with VIDAS and ELISA from the foods of concern. The results of the analysis held with Vidas SET2 showed 72 (24%) of 300 samples and with ELISA these enterotoxins showed diff erence in distribution.

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