MUSHAP KURU, METİN ÖĞÜN, RECAİ KULAKSIZ, ABDULSAMED KÜKÜRT, Hasan ORAL

Comparison of Oxidative/Nitrosative Stress, Leptin and Progesterone Concentrations in Pregnant and Non-pregnant Abaza Goats Synchronized with Controlled Internal Drug Release Application

The aim of this study was to determine the oxidative/nitrosative stress, leptin and progesterone concentrations in pregnant and non-pregnant Abaza goats after synchronization with controlled internal drug release (CIDR) during the breeding season. For this purpose, 40 clinically healthy Abaza goats, aged 2-4 years, were intravaginally exposed to CIDR on day 0, and injected with equine chorionic gonadotropin and prostaglandin F2α on day 9 of the experiment. CIDR was removed on day 11. At the end of the experiment, they were monitored for estrus and exposed to fertile males for mating. Blood samples were collected 8 days before synchronization, then on days 0, 11 of CIDR insertion and on mating day. A pregnancy diagnosis was conducted using transrectal ultrasonography 30 day after mating. The blood serum from 40 goats (30 pregnant + 10 non-pregnant) was used for biochemical analyses. Malondialdehyde (MDA), nitric oxide (NO), total oxidant capacity (TOC), total antioxidant capacity (TAC) and endothelial NO synthase (eNOS) activities were significantly higher on mating day in pregnant goats compared to non-pregnant goats (P

Controlled Internal Drug Release Uygulaması İle Senkronizasyon Yapılan Gebe ve Gebe Kalmayan Abaza Keçilerinde Oksidatif/Nitrozatif Stres, Leptin ve Progesteron Konsantrasyonlarının Karşılaştırılması

Bu çalışmanın amacı üreme sezonunda controlled internal drug release (CIDR) ile senkronizyon sonrasında gebe ve gebe olmayan Abaza keçilerinde oksidatif/nitrosatif stres, leptin ve progesteron konsantrasyonlarının belirlenmesidir. Bu amaçla, klinik olarak sağlıklı 2-4 yaşlı 40 Abaza keçisine 0. gün CIDR intravaginal olarak yerleştirildi ve 9. gün equine chorionic gonadotropin ile prostaglandin F2α enjekte edildi. CIDR 11. gün çıkarıldı. Uygulama sonrasında östrus takibi yapılarak keçiler fertil tekeler ile çiftleştirildi. Senkronizasyona başlamadan 8 gün önce, 0. gün, 11. gün ve çiftleşme günü kan alındı. Çiftleşmeden sonraki 30. gün transrektal ultrasonografi ile gebelik muayenesi yapıldı. Biyokimyasal ölçümler için 40 keçiden (30 gebe + 10 gebe olmayan) elde edilen kan serumu kullanıldı. Gebe olan keçilerle gebe olmayan keçiler karşılaştırıldığında malondialdehid (MDA), nitrik oksit (NO), total oksidan kapasite (TOC), total antioksidan kapasite (TAC) ve endotelyal NO sentaz (eNOS) aktiviteleri anlamlı olarak yüksek bulundu (P

PDF

___

Tatemoto H, Muto N, Sunagawa I, Shinjo A, Nakada T: Protection of porcine oocytes against cell damage caused by oxidative stress during in vitro maturation: Role of superoxide dismutase activity in porcine follicular fluid. Biol Reprod, 71, 1150-1157, 2004. DOI: 10.1095/ biolreprod.104.029264

Gao Q, Horvath TL: Cross-talk between estrogen and leptin signaling in the hypothalamus. Am J Physiol Endocrinol Metab, 294, E817-E826, 2008. DOI: 10.1152/ajpendo.00733.2007

Watanobe H: Leptin directly acts within the hypothalamus to stimulate gonadotropin-releasing hormone secretion in vivo in rats. J Physiol, 545 (1): 255-268, 2002. DOI: 10.1113/jphysiol.2002.023895

Meli R, Pacilio M, Raso GM, Esposito E, Coppola A, Nasti A, Di Carlo C, Nappi C, Di Carlo R: Estrogen and raloxifene modulate leptin and its receptor in hypothalamus and adipose tissue from ovariectomized rats. Endocrinol, 145, 3115-3121, 2004. DOI: 10.1210/en.2004-0129

Thorn SR, Meyer MJ, Van Amburgh ME, Boisclair YR: Effect of estrogen on leptin and expression of leptin receptor transcripts in prepubertal dairy heifers. J Dairy Sci, 90, 3742-3750, 2007. DOI: 10.3168/ jds.2007-0009

Fantuzzi G, Faggioni R: Leptin in the regulation of immunity, inflammation, and hematopoiesis. J Leukoc Biol, 68, 437-446, 2000.

Lago R, Gómez R, Lago F, Gómez-Reino J, Gualillo O: Leptin beyond body weight regulation-current concepts concerning its role in immune function and inflammation. Cell Immunol, 252, 139-145, 2008. DOI: 10.1016/j.cellimm.2007.09.004

Dixon AB, Knights M, Pate JL, Lewis PE, Inskeep EK: Reproductive performance of ewes after 5-day treatment with intravaginal inserts containing progesterone in combination with injection of prostaglandin F2α. Reprod Domest Anim, 41, 142-148, 2006. DOI: 10.1111/j.1439- 0531.2006.00656.x

Wheaton JE, Carlson KM, Windels HF, Johnston LJ: CIDR: A new progesterone-releasing intravaginal device for induction of estrus and cycle control in sheep and goats. Anim Reprod Sci, 33, 127-141, 1993. DOI: 10.1016/0378-4320(93)90111-4

Talukder S, Ingenhoff L, Kerrisk KL, Celi P: Plasma oxidative stress biomarkers and progesterone profiles in a dairy cow diagnosed with an ovarian follicular cyst. Vet Q, 34, 113-117, 2014. DOI: 10.1080 /01652176.2014.953264

Rupnow HL, Phernetton TM, Shaw CE, Modrick ML, Bird IM, Magness RR: Endothelial vasodilator production by uterine and systemic arteries. VII. Estrogen and progesterone effects on eNOS. Am J Physiol Heart Circ Physiol, 280, H1699-H1705, 2001. DOI: 10.1152/ ajpheart.2001.280.4.H1699

Penna B, Libonati H, Director A, Sarzedas AC, Martins G, Brandão FZ, Fonseca J, Lilenbaum W: Progestin-impregnated intravaginal sponges for estrus induction and synchronization influences on goats vaginal flora and antimicrobial susceptibility. Anim Reprod Sci, 142, 71-74, 2013. DOI: 10.1016/j.anireprosci.2013.09.006

Fujii J, Iuchi Y, Okada F: Fundamental roles of reactive oxygen species and protective mechanisms in the female reproductive system. Reprod Biol Endocrinol, 3: 43, 2005. DOI: 10.1186/1477-7827-3-43

Erel Ö: A new automated colorimetric method for measuring total oxidant status. Clin Biochem, 38, 1103-1111, 2005. DOI: 10.1016/j. clinbiochem.2005.08.008

Erel Ö: A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable abts radical cation. Clin Biochem, 37, 277-285, 2004. DOI: 10.1016/j.clinbiochem. 2003.11.015

Miranda KM, Espey MG, Wink DA: A rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite. Nitric Oxide, 5, 62-71, 2001. DOI: 10.1006/niox.2000.0319

Yoshioka T, Kawada K, Shimada T, Mori M: Lipid peroxidation in maternal and cord blood and protective mechanism against activatedoxygen toxicity in the blood. Am J Obstet Gynecol, 135, 372-376, 1979. DOI: 10.1016/0002-9378(79)90708-7

Kuru M, Oral H, Kulaksız R: Determination of gestational age in Abasian and Georgian goats where some embryonic and fetal parameters were measured ultrasonography. p.193. In, 12th International Conference on Goats, Antalya, Turkey, 2016.

Sarraf P, Frederich RC, Turner EM, Ma G, Jaskowiak NT, Rivet DJ, Flier JS, Lowell BB, Fraker DL, Alexander HR: Multiple cytokines and acute inflammation raise mouse leptin concentrations: Potential role in inflammatory anorexia. J Exp Med, 185, 171-175, 1997. DOI: 10.1084/ jem.185.1.171

Maeso Fortuny MC, Brito Díaz B, Cabrera de León A: Leptin, estrogens and cancer. Mini Rev Med Chem, 6, 897-907, 2006. DOI: 10.2174/138955706777934973

Batista AM, Gomes WA, Carvalho CCD, Monteiro PLJ Jr, Silva FLM, Almeida FC, Soares PC, Carneiro GF, Guerra MMP: Effect of leptin on in vivo goat embryo production. Reprod Domest Anim, 49, 476-480, 2014. DOI: 10.1111/rda.12314

Barbieri A, Palma G, Rosati A, Giudice A, Falco A, Petrillo A, Petrillo M, Bimonte S, Di Benedetto M, Esposito G, Stiuso P, Abbruzzese A, Caraglia M, Arra C: Role of endothelial nitric oxide synthase (eNOS) in chronic stress-promoted tumour growth. J Cell Mol Med, 16, 920-926, 2012. DOI: 10.1111/j.1582-4934.2011.01375.x

Agarwal A, Gupta S, Sharma RK: Role of oxidative stress in female reproduction. Reprod Biol Endocrinol, 3, 28, 2005. DOI: 10.1186/1477- 7827-3-28

Sönmez M, Bozkurt T, Türk G, Gür S, Kizil M, Yüce A: The effect of vitamin E treatment during preovulatory period on reproductive performance of goats following estrous synchronization using intravaginal sponges. Anim Reprod Sci, 114, 183-192, 2009. DOI: 10.1016/j. anireprosci.2008.09.007

Ozcan A, Ogun M: Biochemistry of reactive oxygen and nitrogen species. In, Gowder SJT (Ed): Basic principles and clinical significance of oxidative stress. 38-58, IntechOpen, Croatia, 2015. DOI: 10.5772/61193

Oral H, Öğün M, Kuru M, Kaya S: Evaluation of certain oxidative stress parameters in heifers that were administered short term PRID. Kafkas Univ Vet Fak Derg, 21, 569-573, 2015. DOI: 10.9775/kvfd.2015.12969

Kuru M, Merhan O, Kaya S, Oral H, Kukurt A: The effect of short term progesterone-releasing intravaginal device treatment on acute inflamation markers for Holstein heifers. Rev Med Vet, 166, 336-340, 2015.

Kuru M, Kükürt A, Oral H, Öğün M: Clinical use of progesterone and its relation to oxidative stress in ruminants. In, Drevensek G (Ed): Sex Hormones in Neurodegenerative Processes and Diseases. 303-327, IntechOpen, London, 2018. DOI: 10.5772/intechopen.73311

Kuru M, Sogukpinar O, Makav M, Cetin N: Effect of barium selenate injections on fertility of Pirlak ewes subjected to estrus synchronization during non-breeding season. Med Weter, 73, 479-482, 2017. DOI: 10.21521/mw.5758

Kuru M, Kuru Boğa B, Kulaksiz R, Arı UÇ, Oral, H: Effects of the progesterone-based estrus synchronization on some reproductive parameters in Abaza goats. Kocatepe Vet J, 10, 164-171, 2017.

Kaçar C, Kaya S, Kuru, M, Zonturlu AK: Koyun ve keçilerde üremenin denetlenmesinde güncel yöntemler. Turkiye Klinikleri J Vet Sci Obstet Gynecol - Special Topics, 2, 29-37, 2016.