Nikolina NOVAKOV, Miodrag RADINOVIC, Bojana VIDOVIĆ, Milanko ŠEKLER, Dejan VIDANOVIĆ, Gordana NEDELJKOVIĆ, Dragan ROGAN, Aleksandar POTKONJAK

Sırbistan’da Aşılı Sürülerden İzole Edilen Enfeksiyöz Bronşitis Virus Suşlarının Moleküler Karakterizasyonu ve Komşu Ülkelerden İzole Edilen Suşlar İle Karşılaştırılması

Bu çalışmanın amacı, Sırbistan’da Enfeksiyöz bronşitis virus suşlarını izole etmek, genetik karakterizasyonlarını yapmak ve diğer suşlar ile olan filogenetik alakasını belirlemektir. Çalışmada, 13 farklı ticari yumurtacı tavuk sürülerinden trakeal swab yoluyla toplanan toplam 480 örnek kullanıldı. 2016 ile 2017 arasında alınan örneklerin gerçek-zamanlı RT-PCR, multiple nested RT-PCR ve S1 gen sekanslaması ile moleküler analizi gerçekleştirildi. Kısmi S1 sekanslama temelli filogenetik analiz altı suşun D274 genotipi, iki suşun QX genotipi ve iki suşun 4/91 genotipi olduğunu gösterdi. D274 grubuna ait Sırp izolatları arasında nükleotid benzerliğindeki farklılık 0 ile %1.2 arasında değişti. Elde edilen suşlar ile D274 (X15832) karşılaştırıldığında 0 ile %0.9 arasında farklılık tespit edildi. Belirlenen QX suşlarının en yüksek nükleotid benzerliği %98.8 ile %99.1 arasında olmak üzere Çin QXIBV (KC795604) ileydi. 4/91 gentopine ait iki Sırp suşu ile aşı suşu olan 4/91 (KF377577) arasında %99.7 ile %98.8 nükleotid benzerliği gözlemlendi. Bu çalışma D274, QX ve 4/91 genotiplerine ait virusların 2016 ile 2017 yılları arasında Sırbistan’daki kanatlı kümeslerinde bulunduğunu göstermiştir.

Molecular Characterization of Infectious Bronchitis Virus Strains Isolated from Vaccinated Flocks in Serbia and Their Comparison with the Isolated Strains from Neighboring Countries

The aim of this study was to isolate, genetically characterize and determine phylogenetic relationship of strains detected in Serbia incomparison to other strains. A total of 480 samples collected from 13 different commercial layer flocks, obtained from tracheal swabs wereincluded. Samples taken from 2016 to 2017 were molecularly analyzed by real-time RT-PCR, multiplex nested RT-PCR, and by sequencingof the S1 gene. Phylogenetic analyses based on partial S1 sequences revealed that six strains were classified as the D274 genotype, twostrains as the QX genotype and two strains as the 4/91 genotype. The difference in nucleotide similarity between the Serbian isolatesbelonging to the D274 group ranges from 0 to 1.2%. Comparison of the obtained strains and D274 (X15832) showed differences from0 to 0.9%. The greatest nucleotide similarity of detected QX strains was with Chinese QXIBV (KC795604), ranging from 98.8% to 99.1%.Two Serbian strains belonging to the 4/91 genotype had 99.7% and 98.8% nucleotide similarities with vaccine strain 4/91 (KF377577).This study has shown that viruses belonging to D274, QX, and 4/91 genotypes were circulating in poultry flocks in Serbia during 2016and 2017.

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