ALİ ÇALIK, Sakine YALÇIN, Seher KÜÇÜKERSAN, Pınar SAÇAKLI, Gültekin YILDIZ, Muhammad Shazaib RAMAY, Ozan AHLAT, Fatma Kübra ERBAY ELİBOL, Süleyman TABAN

Effects of Calcium Soaps of Animal Fats on Performance, Abdominal Fat Fatty Acid Composition, Bone Biomechanical Properties, and Tibia Mineral Concentration of Broilers

This study evaluated the effects of a graded concentration of dietary calcium soaps of tallow (CST) supplementation on broiler performance, carcass characteristics, abdominal fat fatty acid profile, bone biomechanical properties, and bone mineral composition. One hundred and forty 11-d-old male broiler chickens were randomly allocated to 4 experimental groups with 5 replicate pens containing 7 birds per each. The birds received cornsoybean meal based diet and CST (CST0, CST10, CST20, and CST30, respectively) was included in the grower (0, 10, 20, and 30 g/kg) and finisher (0, 15, 30, and 45 g/kg) diet at the expense of vegetable acid oil and limestone. Dietary supplementations had no significant effect on BWG and FI during the entire experimental period. However, FCR was improved in CST0 and CST10 groups in comparison to those of the CST20 group from d 11 to d 42. According to the present study result, dietary supplementation of low level of CST significantly influenced intestinal microarchitecture of the jejunum and ileum by improving villus height/crypt ratio and villus height, respectively. Femur (P=0.001) and tibia (P=0.020) stiffness increased linearly with the increasing level of dietary CST. Tibia Ca (P=0.009) and P (P=0.009) concentration of CST10 and CST30 groups were lower than the CST0 group. Increasing levels of CST in diets significantly reduced the Fe and Mn concentrations in tibia samples. In conclusion, supplementation of CST has no detrimental effect on broiler performance parameters and might be used as an alternative dietary fat source in the broiler industry.

Hayvansal Yağın Yağ Asitleri Kalsiyum Tuzunun Broyler Performansı, İç Yağın Yağ Asidi Kompozisyonu, Kemiklerin Biyomekanik Özellikleri ve Tibia Mineral Düzeyi Üzerine Etkisi

Bu çalışmada rasyonlara artan düzeylerde ilave edilen hayvansal yağın yağ asiti tuzunun (CST) broylerlerde performans, karkas karakteristikleri, abdominal yağın yağ asiti kompozisyonu, kemiklerin biyomekanik özellikleri ve kemik mineral kompoziyonu üzerine olan etkisi incelenmiştir. Toplamda 140 adet 11 günlük erkek broyler 4 deneme grubuna ve her biri 7 civcivden oluşan 5 tekerrür grubuna rasgele olacak şekilde ayrılmıştır. Broylerler mısır-soyadan oluşan rasyon ile beslenmiştir. Büyütme (0, 10, 20 ve 30 g/kg) ve bitirme (0, 15, 30 ve 45 g/kg) dönemi rasyonları artan düzeylerde CST (CST0, CST10, CST20 ve CST30, sırasıyla) kapsayacak şekilde düzenlenmiştir. Karma yeme ilave edilen yağın, tüm deneme periyodu boyunca, canlı ağırlık artışı ve yem tüketimi üzerine önemli bir etkisi olmadığı görülmüştür. Ancak, araştırmanın 11-42 günleri arasında, CST0 ve CST10 gruplarının CST20 grubu ile karşılaştırıldığında daha iyi yemden yararlanma değerine sahip olduğu görülmüştür. Elde edilen araştırma sonuçlarına göre, karma yeme düşük düzeyde ilave edilen hayvansal yağın jejunum villus yüksekliği/kript derinliği oranını ve ileum villus yüksekliğini arttırmak suretiyle bağırsak histomorfolojisi üzerine olumlu bir etkisi olduğu tespit edilmiştir. Femur (P=0.001) ve tibia (P=0.020) sertliği rasyonlara artan düzeyde CST ilavesi ile birlikte doğrusal bir artış göstermiştir. CST10 ve CST30 gruplarının tibia Ca (P=0.009) ve P (P=0.009) konsantrasyonu, CST0 grubu ile karşılıştırıldığında daha düşük bulunmuştur. Karma yemde CST düzeyinin artması, tibia örneklerindeki Fe ve Mn konstrasyonunu önemli ölçüde azaltmıştır. Sonuç olarak, rasyonlara ilave edilen hayvansal yağın yağ asiti tuzlarının broyler performansı üzerine olumsuz bir etkisi olmadığı ve ucuz bir alternatif yağ kaynağı olarak kullanılabileceği görülmüştür.

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1. NRC: Nutrient Requirements of Poultry. Washington, DC: National Academies Press. 1994.

2. Ravindran V, Tancharoenrat P, Zaefarian F, Ravindran G: Fats in poultry nutrition: Digestive physiology and factors influencing their utilisation. Anim Feed Sci Technol, 213, 1-21, 2016. DOI: 10.1016/j.anifeedsci.2016.01.012

3. Poorghasemi M, Seidavi A, Qotbi AAA, Laudadio V, Tufarelli V: Influence of dietary fat source on growth performance responses and carcass traits of broiler chicks. Asian-Australas J Anim Sci, 26 (5): 705-710, 2013. DOI: 10.5713/ajas.2012.12633

4. Mateos GG, Sell JL: Nature of the extrametabolic effect of supplemental fat used in semipurified diets for laying hens. Poult Sci, 60 (8): 1925-1930, 1981. DOI: 10.3382/ps.0601925

5. Mateos GG, Sell JL, Eastwood JA: Rate of food passage (transit time) as influenced by level of supplemental fat. Poult Sci, 61 (1): 94-100, 1982. DOI: 10.3382/ps.0610094

6. Kim JH, Seo S, Kim CH, Kim JW, Lee BB, Lee GI, Shin HS, Kim MC, Kil DY: Effect of dietary supplementation of crude glycerol or tallow on intestinal transit time and utilization of energy and nutrients in diets fed to broiler chickens. Livest Sci, 154 (1-3): 165-168, 2013. DOI: 10.1016/j. livsci.2013.03.005

7. Ketels E, De Groote G: Effect of ratio of unsaturated to saturated fatty acids of the dietary lipid fraction on utilization and metabolizable energy of added fats in young chicks. Poult Sci, 68 (11): 1506-1512, 1989. DOI: 10.3382/ps.0681506

8. Sibbald IR: The true metabolizable energy values of mixtures of tallow with either soybean oil or lard. Poult Sci, 57 (2): 473-477, 1978. DOI: 10.3382/ ps.0570473

9. Sibbald IR, Kramer JKG: The effect of the basal diet on the true metabolizable energy value of fat. Poult Sci, 57 (3): 685-691, 1978. DOI: 10.3382/ps.0570685

10. Tancharoenrat P, Ravindran V: Influence of tallow and calcium concentrations on the performance and energy and nutrient utilization in broiler starters. Poult Sci, 93 (6): 1453-1462, 2014. DOI: 10.3382/ps. 2013-03817

11. Blanch A, Barroeta AC, Baucells MD, Puchal F: The nutritive value of dietary fats in relation to their chemical composition. Apparent fat availability and metabolizable energy in two-week-old chicks. Poult Sci, 74 (8): 1335-1340, 1995. DOI: 10.3382/ps.0741335

12. Tancharoenrat P, Ravindran V, Zaefarian F, Ravindran G: Influence of age on the apparent metabolisable energy and total tract apparent fat digestibility of different fat sources for broiler chickens. Anim Feed Sci Technol, 186 (3-4): 186-192, 2013. DOI: 10.1016/j.anifeedsci.2013.10.013

13. Sanz M, Flores A, Lopez-Bote CJ: The metabolic use of energy from dietary fat in broilers is affected by fatty acid saturation. Br Poult Sci, 41 (1): 61-68, 2000. DOI: 10.1080/00071660086411

14. Newman RE, Bryden WL, Fleck E, Ashes JR, Storlien LH, Downing JA: Dietary n-3 and n-6 fatty acids alter avian metabolism: molecular-species composition of breast-muscle phospholipids. Br J Nutr, 88 (1): 19-28, 2002. DOI: 10.1079/BJN2002581

15. Salinas J, Ramírez RG, Domínguez MM, Reyes-Bernal N, Trinidad- Lárraga N, Montaño MF: Effect of calcium soaps of tallow on growth performance and carcass characteristics of Pelibuey lambs. Small Rumin Res, 66 (1-3): 135-139, 2006. DOI: 10.1016/j.smallrumres.2005.07.058

16. Rising R, Maiorino PM, Mitchell R, Reid BL: The utilization of calcium soaps from animal fat by laying hens. Poult Sci, 69 (5): 768-773, 1990. DOI: 10.3382/ps.0690768

17. Aviagen: Ross broiler Management Handbook. Huntsville, AL, Aviagen Group, 2014.

18. AOAC: Official Methods of Analysis of AOAC International. Gaithersburg, MD. 2000.

19. Carpenter KJ, Clegg KM: The metabolizable energy of poultry feeding stuffs in relation to their chemical composition. J Sci Food Agr, 7 (1): 45-51, 1956. DOI: 10.1002/jsfa.2740070109

20. Calik A, Ekim B, Bayraktaroğlu AG, Ergün A, Saçaklı P: Effects of dietary probiotic and synbiotic supplementation on broiler growth performance and intestinal histomorphology. Ankara Univ Vet Fak Derg, 64, 183-189, 2017. DOI: 10.1501/Vetfak_0000002797

21. Luna LG: Manual of histologic staining methods of the Armed Forces Institute of Pathology. Newyork: McGraw-Hill Book Co., 1968.

22. Xu ZR, Hu CH, Xia MS, Zhan XA, Wang MQ: Effects of dietary fructooligosaccharide on digestive enzyme activities, intestinal microflora and morphology of male broilers. Poult Sci, 82 (6): 1030-1036, 2003. DOI: 10.1093/ps/82.6.1030

23. Sakamoto K, Hirose H, Onizuka A, Hayashi M, Futamura N, Kawamura Y, Ezaki T: Quantitative study of changes in intestinal morphology and mucus gel on total parenteral nutrition in rats. J Surg Res, 94 (2): 99-106, 2000. DOI: 10.1006/jsre.2000.5937

24. Gürgül S, Erdal N, Yılmaz ŞN, Yıldız A, Ankaralı H: Deterioration of bone quality by long-term magnetic field with extremely low frequency in rats. Bone, 42 (1): 74-80, 2008. DOI: 10.1016/j.bone.2007.08.040

25. Boss CB, Fredeen KJ: Concepts, instrumentation and techniques in inductively coupled plasma optical emission spectrometry. 710 Bridgep Ort. Avenue Shelton, CT 06484–04794 USA: PerkinElmer Life and Analytical Sciences. 2004.

26. SPSSInc: SPSS 14.0.1 for Windows, user’s guide: SPSS Inc. Chicago, IL. 2004.

27. Preston CM, McCracken KJ, Bedford MR: Effect of wheat content, fat source and enzyme supplementation on diet metabolisability and broiler performance. Br Poult Sci, 42 (5): 625-632, 2001. DOI: 10.1080/ 00071660120088443

28. Atteh JO, Leeson S: Effects of dietary fatty acids and calcium levels on performance and mineral metabolism of broiler chickens. Poult Sci, 62 (12): 2412-2419, 1983. DOI: 10.3382/ps.0622412

29. Atteh JO, Leeson S: Effects of dietary saturated or unsaturated fatty acids and calcium levels on performance and mineral metabolism of broiler chicks. Poult Sci, 63 (11): 2252-2260, 1984. DOI: 10.3382/ps.0632252

30. Griffiths L, Leeson S, Summers JD: Fat deposition in broilers: Effect of dietary energy to protein balance, and early life caloric restriction on productive performance and abdominal fat pad size. Poult Sci, 56 (2): 638- 646, 1977. DOI: 10.3382/ps.0560638

31. Sanz M, Flores A, Perez De Ayala P, Lopez-Bote CJ: Higher lipid accumulation in broilers fed on saturated fats than in those fed on unsaturated fats. Br Poult Sci, 40 (1): 95-101, 1999. DOI: 10.1080/00071669987908

32. Leeson S, Summers JD: Production and carcass characteristics of the broiler chicken. Poult Sci, 59 (4): 786-798, 1980. DOI: 10.3382/ps.0590786

33. Wongsuthavas S, Yuangklang C, Vasupen K, Mitchaothai J, Srenanual P, Wittayakun S, Beynen AC: Assessment of de-novo fatty acid synthesis in broiler chickens fed diets containing different mixtures of beef tallow and soybean oil. Int J Poult Sci, 6 (11): 800-806, 2007. DOI: 10.3923/ ijps.2007.800.806

34. Potença A, Murakami AE, Fernandes JIM, Matsushita M, Nakagawa EL: Performance, abdominal fat deposition and bone characteristics of broilers fed diets containing different lipid sources. Rev Bras Cienc Avic, 10 (4): 239-244, 2008. DOI: 10.1590/S1516-635X2008000400008

35. Scaife JR, Moyo J, Galbraith H, Michie W, Campbell V: Effect of different dietary supplemental fats and oils on the tissue fatty acid composition and growth of female broilers. Br Poult Sci, 35 (1): 107-118, 1994. DOI: 10.1080/00071669408417675

36. Zollitsch W, Knaus W, Aichinger F, Lettner F: Effects of different dietary fat sources on performance and carcass characteristics of broilers. Anim Feed Sci Technol, 66 (1-4): 63-73, 1997. DOI: 10.1016/S0377-8401(96)01126-1

37. Crespo N, Esteve-Garcia E: Dietary fatty acid profile modifies abdominal fat deposition in broiler chickens. Poult Sci, 80 (1): 71-78, 2001. DOI: 10.1093/ps/80.1.71

38. Calik A, Ergun A: Effect of lactulose supplementation on growth performance, intestinal histomorphology, cecal microbial population, and short-chain fatty acid composition of broiler chickens. Poult Sci, 94 (9): 2173-2182, 2015. DOI: 10.3382/ps/pev182

39. Sagher FA, Dodge JA, Johnston CF, Shaw C, Buchanan KD, Carr KE: Rat small intestinal morphology and tissue regulatory peptides: Effects of high dietary fat. Br J Nutr, 65 (1): 21-28, 1991. DOI: 10.1079/BJN19910062

40. Li DF, Thaler RC, Nelssen JL, Harmon DL, Allee GL, Weeden TL: Effect of fat sources and combinations on starter pig performance, nutrient digestibility and intestinal morphology. J Anim Sci, 68 (11): 3694-3704, 1990. DOI: 10.2527/1990.68113694x

41. Khatun J, Loh TC, Akit H, Foo HL, Mohamad R: Influence of different sources of oil on performance, meat quality, gut morphology, ileal digestibility and serum lipid profile in broilers. J Appl Anim Res, 46 (1): 479- 485, 2018. DOI: 10.1080/09712119.2017.1337580

42. Shim MY, Karnuah AB, Mitchell AD, Anthony NB, Pesti GM, Aggrey SE: The effects of growth rate on leg morphology and tibia breaking strength, mineral density, mineral content, and bone ash in broilers. Poult Sci, 91 (8): 1790-1795, 2012. DOI: 10.3382/ps.2011-01968

43. Wohl GR, Loehrke L, Watkins BA, Zernicke RF: Effects of high-fat diet on mature bone mineral content, structure, and mechanical properties. Calcif Tissue Int, 63 (1): 74-79, 1998. DOI: 10.1007/s002239900492

44. Zhong X, Gao S, Wang JJ, Dong L, Huang J, Zhang LL, Wang T: Effects of linseed oil and palm oil on growth performance, tibia fatty acid and biomarkers of bone metabolism in broilers. Br Poult Sci, 55 (3): 335-342, 2014. DOI: 10.1080/00071668.2014.891097

45. Currey JD: Physical characteristics affecting the tensile failure properties of compact bone. J Biomech, 23 (8): 837-844, 1990. DOI: 10.1016/0021- 9290(90)90030-7

46. Turner CH: Biomechanics of bone: Determinants of skeletal fragility and bone quality. Osteoporos Int, 13 (2): 97-104, 2002. DOI: 10.1007/ s001980200000

47. Forestier-Zhang L, Bishop N: Bone strength in children: Understanding basic bone biomechanics. Arch Dis Child Educ Pract Ed, 101 (1): 2-7, 2016. DOI: 10.1136/archdischild-2015-308597

48. Lories RJU, Peeters J, Bakker A, Tylzanowski P, Derese I, Schrooten J, Thomas JT, Luyten FP: Articular cartilage and biomechanical properties of the long bones in Frzb‐knockout mice. Arthritis Rheum, 56 (12): 4095- 4103, 2007. DOI: 10.1002/art.23137

49. Kim WK, Bloomfield SA, Ricke SC: Effects of age, vitamin D3, and fructooligosaccharides on bone growth and skeletal integrity of broiler chicks. Poult Sci, 90 (11): 2425-2432, 2011. DOI: 10.3382/ps.2011-01475

50. Rath NC, Huff GR, Huff WE, Balog JM: Factors regulating bone maturity and strength in poultry. Poult Sci, 79 (7): 1024-1032, 2000. DOI: 10.1093/ps/79.7.1024

51. Rath NC, Balog JM, Huff WE, Huff GR, Kulkarni GB, Tierce JF: Comparative differences in the composition and biomechanical properties of tibiae of seven- and seventy-two-week-old male and female broiler breeder chickens. Poult Sci, 78 (8): 1232-1239, 1999. DOI: 10.1093/ ps/78.8.1232