Ahmad RAZA, Saira BASHIR, Romana TABASSUM

Evaluation of Cellulases and Xylanases Production from Bacillus spp. Isolated from Buffalo Digestive System

Cellulases and xylanases have high industrial demand due to their paramount importance in biological processes. The present study was aimed to evaluate the fiber degrading potential of Bacillus spp. isolated from buffalo digestive system. A total of fourteen isolates from rumen and eight isolates from dung were screened on carboxymethyl cellulose (CMC; 1%) agar plates, showing clear zone of CMC hydrolysis. All screened isolates were confirmed by targeting the 16S rRNA gene, sequencing and phylogenetic analysis. The enzyme activity index (EAI) of all screened isolates was calculated and it was observed that BR28 (Bacillus subtilis), BR96 (Bacillus amyloliquefaciens), BD69 (Bacillus tequilensis) and BD92 (Bacillus sonorensis) exhibit 2.11, 2.05, 2.56 and 2.45 EAI, respectively. The isolates with high EAI (>2) were selected for further enzyme production studies. The results of enzyme activities showed that BD92 had higher endoglucanse (carboxymethyl-cellulase, CMCase, 240.76±4.12 U/L) and avicelase (153.56±7.28 U/L) activities after 72 h of incubation. However, BR96 showed highest xylanase (3379.27±10.58 U/L) activity than others isolates and previously reported studies. This study provides a helpful insight into the identification of Bacillus spp. from the buffalo digestive system with higher production of cellulases and xylanases for their application in the animal feed industry.

Bizon Sindirim Sisteminden İzole Edilen Bacillus spp.’den Selülaz ve Ksilanaz Üretiminin Değerlendirilmesi

Selüloz ve ksilanaza biyolojik süreçlerdeki önemlerinden dolayı yüksek endüstriyel talep vardır. Bu çalışmanın amacı, bizon sindirim sisteminden izole edilen Bacillus spp.’nin lifleri parçalama potansiyelinin değerlendirilmesidir. Rumenden on dört ve dışkıdan sekiz izolat karboksimetil selüloz (CMC; %1) agar plakasında CMC hidrolizin açık renkli bölge gösterme durumu bakımından incelendi. İncelenen tüm izolatlar 16S rRNA gen hedeflemesi, sekans ve filogenetik analizlerle onaylandı. İncelenen izolatların tümünün enzim aktivite endeksi (EAE) hesaplandı ve BR28 (Bacillus subtilis), BR96 (Bacillus amyloliquefaciens), BD69 (Bacillus tequilensis) ve BD92 (Bacillus sonorensis)’un sırasıyla 2.11, 2.05, 2.56 ve 2.45 EAE gösterdiği belirlendi. Yüksek (>2) EAE değerine sahip olan izolatlar daha sonra enzim üretim çalışmalarında değerlendirildi. Enzim aktiviteleri, 72 saat inkübasyon sonrasında BD92’nin daha yüksek endoglukanaz (Karboksimetil selülaz, 240.76±4.12 U/L) ve aviselaz (153.56±7.28 U/L) aktivitelerine sahip olduğunu gösterdi. Ancak, BR96 diğer izolat ve daha önceden rapor edilenlerden daha yüksek ksilanaz (3379.27±10.58 U/L) aktivitesine sahipti. Bu çalışma, hayvancılık endüstrisinde uygulama bulabilecek bizon sindirim sisteminden. yüksek düzeyde selülaz ve ksilanaz üretebilecek Bacillus spp. üretimine faydalı bir bakış sunmaktadır.

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