Saeed AHMED, Ijaz AHMAD, Shakoor AHMAD, Zahir SHAH, Muqadar SHAH, Farhan Anwar KHAN

Analysis of Pilus Biogenesis Genes in Bacteria Expressing Type IV Pili

The structural and sequences similarities of type-IV pili proteins were analysed in Pseudomonas aeruginosa, Vibrio cholerae, Neisseria meningitidis and Neisseria gonorrhoeae. Pathogenic Neisseria species possess pili-genes that code structural and assembly proteins of type- IV pili. A collection of assembly proteins reveal homology amongst P. aeruginosa and Neisseria. While in V. cholerae, these proteins ensure functional counterparts. The pilus retraction and assembly ATPases, PilB/PilF and PilT, PilU are homologous in Neisseria and P. aeruginosa, whereas V. cholerae holds only one ATPase called TcpT. In this work only type-IV pilus machinery of P. aeruginosa shows high likeness with pathogenic Neisseria.

Tip IV Pili Eksprese Eden Bakterilerde Pilus Biogenez Genlerinin Analizi

Pseudomonas aeruginosa, Vibrio cholerae, Neisseria meningitidis ve Neisseria gonorrhoeae bakterilerinde Tip IV pili proteinlerinin yapısal ve sekans benzerlikleri incelendi. Patojenik Neisseria türleri tip IV pilinin yapısal ve montaj proteinlerini kodlayan pili genlerine sahiptir. Bir grup montaj proteinleri P. aeruginosa ve Neisseria arasında homoloji göstermektedir. V. cholerae’da ise bu proteinler fonksiyonel karşılıkları sağlamaktadır. Pilus retraksiyon ve montaj ATPaz’ları, PilB/PilF ve PilT, PilU Neisseria ve P. aeruginosa’da homolog olup V. cholera sadece TcpT isimli bir ATPaz’a sahiptir. Bu çalışmada sadece P. aeruginosa’nın tip-IV pilus organeli patojenik Neisseria ile yüksek benzerlik göstermiştir.

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1. Lory S, Strom MS: Structure-function relationship of type-IV prepilin peptidase of P. aeruginosa- A review. Gene, 192, 117-121, 1997. DOI: 10.1016/ S0378-1119(96)00830-X

2. Giltner CL, Nguyen Y, Burrows LL: Type IV pilin proteins: Versatile molecular modules. Microbiol Mol Biol Rev, 76, 740-772, 2012. DOI: 10.1128/ MMBR.00035-12

3. Peabody CR, Chung YJ, Yen MR, Vidal-Ingigliardi D, Pugsley AP, Saier MH: Type II protein secretion and its relationship to bacterial type IV pili and archaeal flagella. Microbiology, 149, 3051-3072, 2003. DOI: 10.1099/ mic.0.26364-0

4. Yildiz FH, Visick KL: Vibrio biofilms: So much the same yet so different. Trends Microbiol, 17, 109-118, 2009. DOI: 10.1016/j.tim.2008.12.004

5. Carbonnelle E, Helaine S, Nassif X, Pelicic V: A systematic genetic analysis in Neisseria meningitidis defines the Pil proteins required for assembly, functionality, stabilization and export of type IV pili. Mol Microbiol, 61, 1510- 1522, 2006. DOI: 10.1111/j.1365-2958.2006.05341.x

6. Brissac T, Mikaty G, Dumenil G, Coureuil M, Nassif X: The meningococcal minor pilin pilX is responsible for type IV pilus conformational changes associated with signaling to endothelial cells. Infect Immun, 80, 3297-3306, 2012. DOI: 10.1128/IAI.00369-12

7. Jain S, Moscicka KB, Bos MP, Pachulec E, Stuart MCA, Keegstra W, Boekema, EJ, van der Does C: Structural characterization of outer membrane components of the type IV pili system in pathogenic Neisseria. PLoS One, 6(1):e16624, 2011. DOI: 10.1371/journal.pone.0016624

8. Chattopadhyaya R, Ghose AC: Model of V. cholerae toxin coregulated pilin capable of filament formation. Protein Eng, 15, 297-304, 2002.

9. Strom MS, Lory S: Structure-function and biogenesis of the type IV pili. Annu Rev Microbiol, 47, 565-596, 1993. DOI: 10.1146/annurev.mi.47.100193.003025

10. Bose N, Taylor RK: Identification of a TcpC-TcpQ outer membrane complex involved in the biogenesis of the toxin-coregulated pilus of Vibrio cholerae. J Bacteriol, 187, 2225-2232, 2005. DOI: 10.1128/JB.187.7.2225-2232.2005

11. Belete B, Lu H, Wozniak DJ: Pseudomonas aeruginosa AlgR regulates type IV pilus biosynthesis by activating transcription of the fimU-pilVWXY1Y2E operon. J Bacteriol, 190, 2023-2030, 2008. DOI: 10.1128/JB.01623-07

12. Nguyen Y, Sugiman-Marangos S, Harvey H, Bell SD, Charlton CL, Junop MS, Burrows LL: Pseudomonas aeruginosa minor pilins prime type IVa pilus assembly and promote surface display of the PilY1 adhesin. J Biol Chem, 290, 601-611, 2005. DOI: 10.1074/jbc.M114.616904

13. Cehovin A, Simpson PJ, McDowell MA, Brown DR, Noschese R, Pallett M, Brady J, Baldwin GS, Lea SM, Matthews SJ, Pelicic V: Specific DNA recognition mediated by a type IV pilin. Proc Natl Acad Sci USA, 110, 3065-3070, 2013. DOI: 10.1073/pnas.1218832110

14. Hélaine S, Carbonnelle E, Prouvensier L, Beretti JL, Nassif X, Pelicic V: PilX, a pilus-associated protein essential for bacterial aggregation, is a key to pilus-facilitated attachment of Neisseria meningitidis to human cells. Mol Microbiol, 55, 65-77, 2005. DOI: 10.1111/j.1365-2958.2004.04372.x

15. Fullner KJ, Mekalanos JJ: Genetic characterization of a new type IV-A pilus gene cluster found in both classical and el tor biotypes of Vibrio cholerae. Infect Immun, 67, 1393-1404, 1999.