Analysis of Pilus Biogenesis Genes in Bacteria Expressing Type IV Pili

The structural and sequences similarities of type-IV pili proteins were analysed in Pseudomonas aeruginosa, Vibrio cholerae, Neisseria meningitidis and Neisseria gonorrhoeae. Pathogenic Neisseria species possess pili-genes that code structural and assembly proteins of type- IV pili. A collection of assembly proteins reveal homology amongst P. aeruginosa and Neisseria. While in V. cholerae, these proteins ensure functional counterparts. The pilus retraction and assembly ATPases, PilB/PilF and PilT, PilU are homologous in Neisseria and P. aeruginosa, whereas V. cholerae holds only one ATPase called TcpT. In this work only type-IV pilus machinery of P. aeruginosa shows high likeness with pathogenic Neisseria.

Tip IV Pili Eksprese Eden Bakterilerde Pilus Biogenez Genlerinin Analizi

Pseudomonas aeruginosa, Vibrio cholerae, Neisseria meningitidis ve Neisseria gonorrhoeae bakterilerinde Tip IV pili proteinlerinin yapısal ve sekans benzerlikleri incelendi. Patojenik Neisseria türleri tip IV pilinin yapısal ve montaj proteinlerini kodlayan pili genlerine sahiptir. Bir grup montaj proteinleri P. aeruginosa ve Neisseria arasında homoloji göstermektedir. V. cholerae’da ise bu proteinler fonksiyonel karşılıkları sağlamaktadır. Pilus retraksiyon ve montaj ATPaz’ları, PilB/PilF ve PilT, PilU Neisseria ve P. aeruginosa’da homolog olup V. cholera sadece TcpT isimli bir ATPaz’a sahiptir. Bu çalışmada sadece P. aeruginosa’nın tip-IV pilus organeli patojenik Neisseria ile yüksek benzerlik göstermiştir.

Kaynakça

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