Tuncer KUTLU, Mehmet Eray ALÇIĞIR, Ekrem Çağatay ÇOLAKOĞLU, Ceyda DİNÇ

Bir Köpekte Küçük Yuvarlak Hücreli Dezmoplastik Tümör

Husky ırkı, 13 yaşlı, kısırlaştırılmış dişi bir köpek, anoreksi, kusma, karın şişliği ve solunum sıkışması şikayetiyle kliniğe getirildi. Hayvana müdahele sırasında aniden öldü. Nekropside karın boşluğunda 16x15x9 cm boyutlarında bir kitleyle karşılaşıldı. Histolojik incelemede hiposelüler, az sayıda mekik şekilli bağ doku hücrelerinden oluşan geniş desmoplastik bağ doku stromasıyla adacıklara ayrılan; hafif eozinofilik dar sitoplazmalı, küçük yuvarlak-oval hiperkromatik çekirdekli, çekirdecikleri fark edilmeyen hücre kümeleri gözlendi. İmmunohistokimyal incelemede tümör hücre sitoplazmalarının nestin yönünden hafif pozitif olduğu saptandı. Tümör hücreleri α-SMA (Alpha-Smooth Muscle Actin), vimentin ve pansitokeratin negatif olup stroma α-SMA ve vimentin pozitifti. İmmunohistokimyasal yönden kısmen farklı bulgular göstermekle beraber gerek lokalizasyonu gerekse histomorfolojik açıdan insanlardakine benzerliklerinden dolayı bu tümöre desmoplastik küçük yuvarlak hücreli tümör teşhisi konmuştur. Daha önce bu tümör hayvanlarda tanımlanmamıştır.

Desmoplastic Small Round-Cell Tumor in a Dog

A 13-year-old, neutered female Husky dog was brought to the clinic with the complaints of anorexia, vomiting, abdominal distensionand respiratory distress. It was suddenly died during intervention. At the necropsy, a mass was seen in the abdominal cavity. The masshad 16x15x9 cm in diameters. Histological examination revealed clusters of cells with slightly eosinophilic and scanty cytoplasm, smallround hyperchromatic nuclei, and inconspicuous nucleoli encompassed by hypocellular extensive desmoplastic connective tissue stromacomprising few spindle-shaped connective tissue cells. In immunohistochemical examination the cytoplasms of tumour cells were detectedto be mildly positive by nestin. The tumour cells were negative for α-SMA (Alpha-Smooth Muscle Actin), vimentin and pancytokeratin, butstromal cells were positive for α-SMA and vimentin. Despite the presence of partially incompatible immunohistochemical findings, the tumorin this case was diagnosed as desmoplastic small round-cell tumor because its aggressiveness, localization, and histopathology was similarto that observed for this tumor in humans. Previously this tumor has not been identified in animals.

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