Ling YANG, Fang TANG, Lan LIU, Weimin ZHAO, Yiting TANG, Ying CHENG, Jingliang WEI, Shulin YANG, Yulian MU, Shutang FENG, Kui LI

Effect of knockdown TAGLN on the migration capacity of wuzhishan pig's bone marrow mesenchymal stem cells

Mezenkimal kök hücreleri (MSCs) kemik iliğinden kolayca izole edilebilen ve takibinde in vitro olarak çoğalabilen mültipotent olgun kök hücreleridir. Kemik iliği MSC (BMMSC)lerinin zayıf immunojenik özellikte olduğu görülmekte olup doku yenileme tedavisinde en sıklıkla kullanılan MSClerdir ve BMMSClerin göç etme kapasiteleri yenileme tedavisindeki başarının anahtarıdır. Wuzhishan domuzu (WZSP) fizyolojik ve genel biyokimyasal belirtileri açısından insana oldukça benzerdir ve boyutu düşünüldüğünde insana organ nakli bakımından oldukça yüksek potansiyele sahiptir. Bu çalışmada WZSPden elde edilen BMMSClerin TAGLN tarafından düzenlenen göç kapasiteleri in vitro olarak çalışıldı. TAGLN için spesifik kısa hairpin RNA (shRNA) TAGLN genini bloke etmek için dizayn edildi. Scratch metodu ve transwell göç metodu bloke TAGLN genin WZSPden elde edilen BMMSClerin göç kapasiteleri üzerine etkisini araştırmak amacıyla uygulandı. Sonuçlar TAGLN genini bloke etmede kullanılan spesifik shRNAnın BMMSC için etkili olduğunu ve in vitro ortamda TAGLN blokajının WZSPden elde edilen BMMSClerin göç kapasiteleri üzerine anlamlı bir etkisinin var olduğunu gösterdi. Sonuç olarak TAGLN BMMSClerde göç kapasitesini sağlamada önemli bir faktördür. Bu durum; WZSPden elde edilen BMMSClerin insanlarda yenileme tedavilerinde kullanılmasına olanak sağlayabilir.

TAGLN Geninin bloke edilmesinin wuzhishan domuz kemik iliği mezenkimal kök hücrelerinde göç etme kapasitesi üzerine etkisi

Mesenchymal stem cells (MSCs) are multipotent adult stem cells, can easily be isolated from the bone marrow and subsequently expand in vitro . Bone marrow MSCs (BMMSCs) appear to be poorly immunogenic, and are the most widely used MSCs in tissue regenerative medicine, and the migration capacity of BMMSCs is the key determinant for the efficiency of their regenerative therapy. The Wuzhishan pig (WZSP) is characterized by its physiological and general biochemical indices very similar to humans, and may be potential organ donors for human being owing to its size. In this study, the BMMSCs from WZSP were used to study their migration capacity which regulated by TAGLN in vitro . The specific short hairpin RNA (shRNA) for TAGLN was designed to knock down TAGLN gene, and the scratch assay and transwell migration assay was employed to estimate efect of knockdown TAGLN on the migration capacity of BMMSCs from WZSP. The results showed that the specific shRNA for knocking down TAGLN efficiently was found for the BMMSCs, and there was a significant efect on the migration capacity of the BMMSCs from WZSP with knockdown TAGLN in vitro . In conclusion, TAGLN was an important factor in maintaining the migration capacity of the BMMSCs, which may be benefit for the BMMSCs from WZSP to be used in regenerative therapy for human being.

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