Ebrahim RAHIMI, Mehdi RAISSY, Morteza RAZZAGHIMANESH, Asiye DASTGERDI AHMADI, Manouchehr SHAHRAKI MOMENI

Occurrence of Aeromonas hydrophila in fish, shrimp, lobster and crab in Iran

Bu çalışma İranın güney kıyılarında yakalanan balık, karides, ıstakoz ve yengeçlerde Aeromonas hydrophila prevalansını ve etkenin antimikrobiyal dayanıklılığını belirlemek amacıyla yapılmıştır. Çalışmada 4 ayrı türden, Otollithes ruber, Pamous argenteus, Parastromateus niger ve Psettodes erumel, toplam 133 balık; 4 ayrı türden, Penaeus monodon, P. semisulcatus, P. indicus, ve P. merguiensis, toplam 240 adet karides; 108 ıstakoz (Panulirus homarus ) ve 60 yengeç (Panulirus homarus ) İranın güney sahillerinde İran Körfezi boyunca 3 bölgeden olmak üzere toplam 541 örnek toplanmıştır. Örnekler Eylül 2011 ile Mayıs 2012 arası her ayın sonunda toplanmıştır. Rutin bakteriyolojik metot ile 66 A. hydrophila izolatı belirlenmiş ve bunların 62si PCR ile A. hydrophila 16S rDNA gen hedefi ile teyit edilmiştir. A. hydrophila sitotoksik enterotoksin gen hedefi ile uygulanan PCR metodu ile 57 (%10.5) izolat pozitif olarak tespit edilmiştir. A. hydrophila prevalansı en yüksek olarak balıklarda (%19.5), sonrasında ise sırasıyla ıstakozlarda (%9.3), karideslerde (%9,2) ve yengeçlerde (%6.7) belirlenmiştir. Mevsimsel olarak en yüksek A. hydrophila prevalansı yaz ayında (%21.3) olurken bunu sırasıyla sonbahar (%12.0), ilkbahar (%10.8) ve kış (%5.6) izledi. Bizim bilgimiz kapsamında bu çalışma İranda balık, karides, ıstakoz ve yengeçlerde A. hydrophila izolasyonunun rapor edildiği ilk çalışmadır.

İranda balık, karides, Istakoz ve yengeçlerde Aeromonas hydrophila mevcudiyeti

This study was conducted to determine the prevalence rate and antimicrobial resistance of Aeromonas hydrophila in fish, shrimp, lobster and crab caught of the south coast of Iran. A total of 541 samples including, 133 freshly caught fish of 4 diferent types including Otollithes ruber, Pamous argenteus, Parastromateus niger and Psettodes erumel, 240 shrimp of 4 diferent species including Penaeus monodon, P. semisulcatus, P. indicus, and P. merguiensis, 108 lobster (Panulirus homarus ) and 60 crab (Panulirus homarus) were collected in 3 provinces along Persian Gulf in the south coast of Iran. Samples were collected at the end of each month from September 2011 to May 2012. Using conventional bacteriological techniques, 66 A. hydrophila isolates were identified in which 62 strains were confirmed by PCR assay targeting 16S rDNA gene of A. hydrophila. Using PCR assays targeting the A. hydrophila cytolytic enterotoxin gene, 57 (10.5%) isolates were positive. The highest prevalence of A. hydrophila was found in fish (19.5%), followed by shrimp (9.2%), lobster (9.3%) and crab (6.7%). The highest prevalence of A. hydrophila occurred in summer (21.3%) followed by fall (12.0%), spring (10.8%), and winter (5.6%). To our knowledge, the present study is the first report of the isolation of A. hydrophila from fish, shrimp, lobster and crab in Iran.

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1. Kirov SM: Aeromonas species. In, Hocking AD (Ed): Foodborne Microorganisms of Public Health Significance. 553-575, AIFST Inc., 2003.
2. Ottaviani D, Santarelli S, Bacchiocchi S, Masini L, Ghittino C, Bacchiocchi I: Occurrence and characterization of Aeromonas spp. in mussels from the Adriatic Sea. Food Microbiol, 23, 418-422, 2006.
3. Hanninen ML, Salmi S, Mattila L, Taipalinen R, Siitonen A: Association of Aeromonas spp. with travellers diarrhoea in Finland. J Med Microbiol, 42, 26-31, 1995.
4. Parker JL, Shaw JG: Aeromonas spp. clinical microbiology and disease. J Infect, 62, 109-118, 2011.
5. Chopra AK, Houston CW: Enterotoxins in Aeromonas associated gastroenteritis. Microbes Infect, 1, 1129-1137, 1999.
6. Pin C, Benito Y, Garcia ML, Selgas D, Tormos J, Casas C: Infuence of temperature, pH, sodium chloride, and sodium nitrite on the growth of clinical and food motile Aeromonas spp. strains. Arch Lebensmittelhygiene , 47, 35-56, 1996.
7. Devlieghere F, Lefevere I, Magnin A, Debevere J: Growth of Aeromonas hydrophila in modified-atmospherepacked cooked meat products. Food Microbiol, 17, 185-196, 2000.
8. Fiorentini C, Barbieri E, Falzano L, Matarrese P, Bafone W, Pianetti A, Katouli M, Kuhn I, Mollby R, Bruscolini F, Casiere A, Donelli G: Occurrence, diversity and pathogenicity of mesophilic Aeromonas in estuarine waters of the Italian coast of the Adriatic Sea. J Appl Bacteriol, 85, 501-511, 1998.
9. Bin Kingombe CI, Huys G, Howald D, Luthi E, Swings J, Jemmi T: The usefulness of molecular techniques to assess the presence of Aeromonas spp. harboring virulence markers in foods. Int J Food Microbiol, 94, 113- 121, 2004.
10. Kariptas E, Erdem B, Görgülü Ö: Protein profiles in diferent strains of Aeromonas hydrophila isolated from retail foods. Kafkas Univ Vet Fak Derg, 15 (6), 885-890, 2009.
11. Vivekanandhan G, Hatha AAM, Lakshmanaperumalsamy P: Prevalence of Aeromonas hydrophila in fish and prawns from the seafood market of Coimbatore, South India. Food Microbiol, 22, 133-137, 2005.
12. P alu´ AP, Gomes LM, Miguel MAL, Balassiano IT, Queiroz MLP, Freitas-Almeida AC, de Oliveir SS: Antimicrobial resistance in food and clinical Aeromonas isolates. Food Microbiol, 23, 504-509, 2006.
13. Illanchezian S, Jayaraman S, Manoharan MS, Valsalam S: Virulance and cytotoxicity of seafood borne Aeromonas hydrophila . Braz J Microbiol, 41, 978-983, 2010.
14. Avsever ML, Polat SH, Türk N, Metin DY: Saprolegnia sp. and Aeromonas hydrophila isolation from freshwater-crayfish (Astacus leptodactylus). Kafkas Univ Vet Fak Derg, 17 (5), 873-875, 2011.
15. Xan thopoulos V, Tzanetakis N, Litopoulou-Tzanetaki E: Occurrence and characterization of Aeromonas hydrophila and Yersinia enterocolitica in minimally processed fresh vegetable salads. Food Control, 21, 393-398, 2010.
16. Sambrook J, Russell DW: Molecular Cloning: A Laboratory Manual. 3 rd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, 2001.
17. Chu WH, Lu CP: Multiplex PCR assay for the detection of pathogenic Aeromonas hydrophila. J Fish Dis, 28, 437-441, 2005.
18. Dorsch M, Ashbolt NJ, Cox PT, Goodman AE: Rapid identification of Aeromonas species using 16S rDNA targeted oligonucleotide primers: A molecular approach based on screening of environmental isolates. J Appl Bacteriol, 77, 722-726, 1994.
19. Chopra AK, Houston CW, Peterson JW, Jin GF: Cloning, expression, and sequence analysis of a cytolytic enterotoxin gene from Aeromonas hydrophila. Can J Microbiol, 39, 513-523, 1993.
20. Radu S, Ahmad N, Ling FH, Reezal A: Prevalence and resistance to antibiotics for Aeromonas species from retail fish in Malaysia. Int J Food Microbiol, 81, 261-266, 2003.
21. Tsai GJ, Chen TW: Incidence and toxigenicity of Aeromonas hydrophila in seafood. Int J Food Microbiol, 31, 121-131, 1996.
22. Hudson JA, De Lacey KM: Incidence of motile aeromonads in New Zealand retail foods. J Food Prot, 54, 696-699, 1991.
23. Durmaz Y, Türk N: Isolation of motil aeromonases and to determine a ntimicrobial susceptibility from farms of trout. Kafkas Univ Vet Fak Derg , 15 (3), 357-361, 2009.
24.Gobat PF, Jemmi T: Distribution of mesophilic Aeromonas species in raw and ready-to-eat fish and meat products in Switzerland. Int J Food Microbiol, 20, 117- 120, 1993.
25. Colakoglu FA, Sarmasik A, Koseoglu B: Occurrence of Vibrio spp. and Aeromonas spp. in shellfish harvested of Dardanelles of Turkey. Food Control, 17, 648-652, 2006.
26. Thayumanavan T, Vivekanandhan G, Savithamani K, Subashkumar R, Lakshmanaperumalsamy P: Incidence of haemolysin-positive and drug-resistant Aeromonas hydrophila in freshly caught finfish and prawn collected from major commercial fishes of coastal South India. FEMS Immunol Med Microbiol, 36, 41-45, 2003.
27. Yogananth N, Bhakyaraj R, Chanthuru A, Anbalagan T, Mullai Nila K: Detection of virulence gene in Aeromonas hydrophila isolated from fish samples using PCR technique. Global J Biotechnol Biochem, 4, 51-53, 2009.
28. Wang C, Silva JL: Prevalence and characterization of Aeromonas species isola ted from processed channel catfish. J Food Prot, 62, 30-34, 1999.