İSMAİL SEVEN, BURCU GÜL BAYKALIR, PINAR TATLI SEVEN, GÜRDAL DAĞOĞLU

The ameliorative efects of propolis against cyclosporine A induced hepatotoxicity and nephrotoxicity in rats

Bu çalışma, Siklosporin A (CsA) uygulanan ratlarda propolisin etkilerini belirlemek amacıyla planlanmıştır. Çalışmada 24 adet Sprague-Dawley erkek rat kullanılmıştır. Ratlar tesadüfi olarak kontrol ve 3 muamele grubuna ayrılmıştır. Grup 1 (Kontrol)e katkı yapılmadı; CsA her gün canlı ağırlığa (BW) 15 mg/kg s.c. olarak verildi (grup 2); Propolis her gün 100 mg/kg BW gastrik gavajla verildi (grup 3); CsA+Propolis her gün 15 mg/kg BW CsA s.c. olarak ve 100 mg/kg BW propolis gastrik gavajla verildi (grup 4). 21 günlük peryottaki yem tüketimi, Kontrol ve Propolis gruplarında CsA ve CsA+Propolis gruplarından önemli derecede daha yüksek oldu (P

Ratlarda hepatotoksisite ve nefrotoksisite oluşturan siklosporin Aya karşı propolisin iyileştirici etkileri

This study was planned to determine the effects of propolis in rats applied Cyclosporine A (CsA). In this study, 24 male Sprague-Dawley rats were used. Rats were randomly divided into 4 groups including control and 3 treatment groups. Group 1 (Control) were no supplement; CsA (group 2) were given as s.c. 15 mg/kg body weight (BW) every day; Propolis (group 3) were given by gavage 100 mg/kg BW every day; CsA+Propolis (group 4) were given as s.c. 15 mg/kg BW of CsA and by gavage 100 mg/kg BW of propolis every day. The feed intake were significantly higher (P<0.01) in Control and Propolis groups than CsA and CsA+Propolis groups within time period of 21 days. Further, body weight was significantly lower (P<0.01) in groups administrated with CsA (Group 2 and 4) than the other groups. Cortisol, AST, ALT and urea levels in serum of Control, Propolis and CsA+Propolis groups were found significantly lower (P<0.01) than those of CsA group. Malondialdehyde levels in kidney and liver tissues were significantly higher (P<0.01) than in the CsA groups compared to other groups. The catalase and reduced glutathione activities in kidney tissue of CsA+Propolis group were significantly higher (P<0.01) than those of CsA group. The present study demonstrated that propolis provided amelioration in terms of hepatotoxicity and nephrotoxicity consisting rats applied to CsA.

PDF

___

1.Sander M, Victor RG: Hypertension after cardiac transplantation: Pathophysiology and management. Curr Opin Nephrol Hy, 4 (5): 443-451, 1995.
2.Xue H, Buroski RD, McCarron DA, Bennett WM: Production of contraction in isolated rat aorta by cyclosporin. Transplantation, 43 (5): 715-718, 1987.
3.Rezzani R, Giugno L, Bufoli B, Bonomini F, Bianchi R: The protective efect of cafeic acid phenethyl ester against cyclosporine A-induced cardiotoxicity in rats. Toxicology, 212 (2-3): 155-164, 2005.
4.Tavares P, Fontes Ribeiro CA, Teixeira F: Cyclosporin effect on noradrenaline release from the sympathetic nervous endings of rat aorta. Pharmacol Res, 47 (1): 27-33, 2003.
5.Kumano K, Yashida K, Iwamura M, End T, Sakai T, Nakamura K, Kuwoo T: The role of reactive oxygen species in cyclosporin A induced nephrotoxicity in rats. Transplantation Proceedings, 21 (1): 941-942, 1989.
6. Tatli Seven P: The efects of dietary Turkish propolis and vitamin C on performance, digestibility, egg production and egg quality in laying hens under diferent environmental temperatures. Asian-Aust J Anim Sci, 21 (8): 1164-1170, 2008.
7. Tatli Seven P, Yilmaz S, Seven I, Cerci IH, Azman MA, Yilmaz M: The efect of propolis on selected blood indicators and antioxidant enzyme activities in broilers under heat stress. Acta Vet Brno , 78, 75-83, 2009.
8.Halliwell B: Free radicals, antioxidants, and human disease: Curiosity, cause, or consequence? Lancet, 344 (8924): 721-724, 1994.
9.Seven I, Tatli Seven P, Yilmaz S: Responses of broilers under cold conditioning (15°C) to dietary triiodothyronine and iodine combined to antioxidants (selenium and vitamin C). Kafkas Univ Vet Fak Derg , 15 (4): 499-504, 2009.
10.Tatli Seven P, Yilmaz S, Seven I, Tuna Kelestemur G: Efects of propolis in animals exposed oxidative stress. In, Lushchak VI (Ed): Oxidative Stress-Environmental Induction and Dietary Antioxidants. 267- 288, TECH BOOK (ISBN: 978-953-51-0553-4), Rijeka, Croatia, 2012. DOI: 10.5772/2536.
11. Burdo ck GA: Review of the biological properties and toxicity of bee propolis (Propolis). Food Chem Toxicol, 36 (4): 347-363, 1998.
12. Banskota AH, Tezuka Y, Adnyana IK, Midorikawa K, Matsushige K, Message D, Huertas AA, Kadota S: Cytotoxic, hepatoprotective and free radical scavenging efects of propolis from Brazil, Peru, the Netherlands and China. J Ethnopharmacol, 72 (1-2): 239-246, 2000.
13.Sathiavelu J, Senapathy GJ, Devaraj R, Namasivayam N: Hepato- protective efect of chrysin on prooxidant-antioxidant status during ethanol-induced toxicity in female albino rats. J Pharm Pharmacol, 61 (6): 809-817, 2009.
14.El-khawaga OA, Salem TA, Elshal MF: Protective role of Egyptian propolis against tumor in mice. Clin Chim Acta , 338 (1-2): 11-16, 2003.
15. Mannaa F, El-Shamy KA, El-Shaikh KA, El-Kassaby M: Efficacy of fish liver oil and propolis as neuroprotective agents in pilocarpine epileptic rats treated with valproate. Pathophysiology, 18 (4): 287-294, 2011.
16.Seo KW, Park M, Song YJ, Kim SJ, Yoon KR: The protective efects of propolis on hepatic injury and its mechanism. Phytother Res, 17 (3): 250-253, 2003.
17. Coneac G, Gafiţanu E, Hădărugă DI, Hădărugă NG, Pînzaru IA, Bandur G, Urşica L, Păunescu V, Gruia A: Flavonoid contents of propolis from the west side of Romania and correlation with the antioxidant activity. Chem Bull POLITEHNICA Univ (Timisoara), 53 (67): 1-2, 2008.
18. Velioglu YS, Mazza G, Gao L, Oomah BD: Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. J Agr Food Chem , 46 (10): 4113-4117, 1998.
19. Kim D, Jeong SW, Lee CY: Antioxidant capacity of phenolic phytochemicals from various cultivars of plums. Food Chemistry, 81 (3): 321-326, 2003.
20. Rai S, Wahile A, Mukherjee K, Saha BP, Mukherjee PK: Antioxidant activity of Nelumbo nucifera (sacred lotus) seeds. J Ethnopharmacol, 104 (3): 322-327, 2006.
21. Apak R, Guclu K, Ozyurek M, Karademir SE: Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method. J Agr Food Chem , 52 (26): 7970-7981, 2004.
22. Ahn MR, Kumazawa S, Usui Y, Nakamura J, Matsuka M, Zhu F, Nakayama T: Antioxidant activity and constituents of propolis collected in various areas of China. Food Chemistry, 101 (4): 1383-1392, 2007.
23.Bino RJ, de Vos CHR, Lieberman M, Hall RD, Bovy A, Jonker HH, Tikunov Y, Lommen A, Moco S, Levin I: The light-hyperresponsive high pigment-2 dg mutation of tomato: Alterations in the fruit metabolome. New Phytologist, 166 (2): 427-438, 2005.
24. Candan N, Tuzmen N: Very rapid quantification of malondialdehyde (MDA) in rat brain exposed to lead, aluminium and phenolic antioxidants by high-performance liquid chromatography-fuorescence detection. NeuroToxicology, 29 (4): 708-713, 2008.
25.Ellman GL: Tissue Sulfhydryl groups. Arch Biochem Biophys, 82 (1): 70-77, 1959.
26. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ: Protein measurement with the folin phenol reagent. J Biol Chem , 193 (1): 265-275, 1951.
27.Aebi H: Catalase in vitro methods in enzymology. In, Willam BJ (Ed): Methods in Enzymology. 121-126, Academic Press. New York, USA, 1984.
28. SPSS: IBM SPSS Statistics 21 CLIENT. IBM Corp. (c) Copyright, 2012.
29.Hagar HH: The protective efect of taurine against cyclosporine A-induced oxidative stress and hepatotoxicity in rats. Toxicol Lett, 151 (2): 335-343, 2004.
30.Andrés D, Sanz N, Zaragoza A, Alvarez AM, Cascales M: Changes in antio xidant defense systems induced by cyclosporine A in cultures of hepatocytes from 2- and 12- month-old rats. Biochem Pharmacol, 59 (9): 1091-1100, 2000.
31.Wolf A, Trendelenburg CF, Diez-Fernandez C, Prieto P, Houy S, Trommer WE, Cordier A: Cyclosporine A-induced oxidative stress in rat hepatocytes. JPET, 280 (3): 1328-1334, 1997.
32. Ca˘ta˘lin Mot A, Silaghi-Dumitrescu R, Sarbu C: Rapid and efective evaluation of the antioxidant capacity of propolis extracts using DPPH bleaching kinetic profiles, FT-IR and UV-vis spectroscopic data. J Food Compos Anal, 24 (4-5): 516522, 2011.
33. Gülçin I, Bursal E, Sehitoğlu MH, Bilsel M, Gören AC: Polyphenol contents and antioxidant activity of lyophilized aqueous extract of propolis from Erzurum, Turkey. Food Chem Toxicol, 48 (8-9): 2227-2238, 2010.
34. Ozyurek M, Guclu K, Apak R: The main and modified CUPRAC methods of antioxidant measurement. TrAC, 30 (4): 652-664, 2011.
35.Phillips A, Wainberg MA, Coates R, Klein M, Rachlis A, Read S, Shepherd F, Vellend H, Walmsley S, Halloran P, Fanning M: Cyclosporine- induced deterioration in patients with AIDS. CMAJ, 140 (12): 1456-1460, 1989.
36.Wongmekiat O, Gomonchareonsiri S, Thamprasert K: Caffeic acid phenethyl ester protects against oxidative stress-related renal dysfunction in rats treated with cyclosporin A. Fund Clin Pharmacol, 25 (5): 619-626, 2011.
37.Tariq M, Morais C, Sobki S, Al Sulaiman M, Al Khader A: N- acetylcysteine attenuates cyclosporin-induced nephrotoxicity in rats. Nephrol Dial Transplant, 14 (4): 923-929, 1999.
38.Wongmekiat O, Thamprasert K: Investigating the protective efects of aged garlic extract on cyclosporin-induced nephrotoxicity in rats. Fundam Clin Pharmacol, 19 (5): 555-562, 2005.
39.Mohamadin AM, El-Beshbishy HA, El-Mahdy MA: Green tea extract attenuates cyclosporine A-induced oxidative stress in rats. Pharmacol Res, 51 (1): 51-57, 2005.
40. Wongmekiat O, Leelarugrayub N, Thamprasert K: Beneficial efect of shallot (Allium ascalonicum L. ) extract on cyclosporin nephrotoxicity in rats. Food Chem Toxicol, 46 (5): 1844-1850, 2008.
41. Shalmany SK, Shivazard M: The efect of diet propolis suplementation on Ross broiler chicks performance. IJPS , 5 (1): 84-88, 2006.
42. Seven I, Aksu T, Tatli Seven P: The efects of propolis and vitamin c supplemented feed on performance, nutrient utilization and carcass characteristics in broilers exposed to lead. Livest Sci, 148 (1-2): 10-15, 2012.
43. Tatli Seven P, Seven I, Yilmaz M, Şimşek ÜG: The efects of Turkish propolis on growth and carcass characteristics in broilers under heat stress. Anim Feed Sci Technol, 146 (1-2): 137-148, 2008.
44. Hirano T, Kawamura T, Fukuda S, Kohsaka S, Yoshikawa N, Yoshida M, Oka K: Implication of cholesterol in cyclosporine pharmacodynamics in minimal change nephrotic syndrome. Clin Pharmacol Ther, 74 (6): 581- 590, 2003.
45.Pari L, Sivasankari R: Efect of ellagic acid on cyclosporine A-induced oxidative damage in the liver of rats. Fundam Clin Pharmacol, 22 (4): 395- 401, 2008.
46.Zhong Z, Li X, Yamashina S, von Frankenberg M, Enomoto N, Ikejima K, Kolinsky M, Raleigh JA, Thurman RG: Cyclosporine A causes a hypermetabolic state and hypoxia in the liver: Prevention by dietary glycine. J Pharmacol Exp Ther, 299 (3): 858-865, 2001.
47. Kim KA, Lee WK, Kim JK, Seo MS, Lim Y, Lee KH, Chae G, Lee SH, Chung Y: Mechanism of refractory ceramic fiber-and rock wool-induced cytotoxicity in alveolar macrophages. Int Arch Occup Environ Health , 74 (1): 9-15, 2001.
48. Priydarsini KI, Khopde SM, Kumar SS, Mohan H: Free radical studies of ellagic acid, a neutral phenolic antioxidant. J Agric Food Chem , 50 (7): 2200-2206, 2002.
49. Mutlu N, Ersan Y, Nur G, Koç E: Protective efect of cafeic acid phenethyl ester against lead acetate-induced hepatotoxicity in mice. Kafkas Univ Vet Fak Derg , 17 (Suppl A): S1-S5, 2011.
50. Yılmaz N, Ilhan S, Nazıroğlu M, Oktar S, Nacar A, Arıca V, Tutanç M: Ceftriaxone ameliorates cyclosporine A-induced oxidative nephrotoxicity in rat. Cell Biochem Funct, 29 (2): 102-107, 2011.
51. Al-Malki AL, Moselhy SS: The protective efect of epicatchin against oxidative stress and nephrotoxicity in rats induced by cyclosporine. Hum Exp Toxicol, 30 (2): 145-151, 2011.
52. Havsteen BH: The biochemistry and medical significance of the favonoids. Pharmacol Ther, 96 (2-3): 67-202, 2002.