YASEMİN ÖNER, ABDÜLKADİR ORMAN, HAKAN ÜSTÜNER, ALPER YILMAZ

Investigation of polymorphisms on ABCG2, AA-NAT and FABP3 genes in the kıvırcık sheep reared in three diferent provinces of Turkey

Bu çalışmada Türkiyenin Bursa, Manisa ve İstanbul illerinden örneklenen Kıvırcık koyunlarında ABCG2 geninin 5.intron, AA-NAT geninin 3. ekzonunda ve FABP3 geninin 2. ekzonunda bulunan mutasyonlar PCR tabanlı yöntemlerle incelenmiştir. İncelenen tüm lokuslar polimorfik bulunmuştur. ABCG2 ve FABP3 lokuslarında iki allel ve üç genotip belirlenirken, AA-NAT lokusunda iki allel ve iki genotip belirlenmiştir. ABCG2 lokusunda - alleli, AA-NAT lokusunda A alleli ve FABP3 lokusunda G allellinin predominant olduğu gözlenmiştir. ABCG2, AA-NAT ve FABP3 lokuslarındaki predominant allelerin frekans değerleri sırasıyla 0.60, 0.87 ve 0.58 olarak bulunmuştur. İncelenen populayonun tamamı ABCG2 ve FABP3 lokusları bakımından Hardy-Weinberg dengesinden sapma gösterirken AA-NAT lokusu bakımından dengede bulunmuştur.

Türkiyenin üç faklı ilinde yetiştirilen kıvırcık koyunlarda ABCG2, AA-NAT ve FABP3 genlerindeki polimorfizmlerin incelenmesi

In this study mutations located in intron 5 of ABCG2, exon 3 of AA-NAT and exon 2 of FAB3 genes were investigated by PCR based methods in the Kıvırcık sheep sampled from Bursa, Manisa, and İstanbul provinces of Turkey. All loci investigated were found as polymorphic. While in the ABCG2 and FABP3 loci two alleles and three genotypes were found, in the AA-NAT locus two alleles and two genotypes were detected. In ABCG2 locus allele - , in AA-NAT allele A, in FABP3 locus allele G were observed as predominant. The frequency values of the predominat alleles at ABCG2, AA-NAT and FABP3 loci were found 0.60, 0.87 and 0.58, respectively. While the whole population investigated exhibits deviation from the Hardy- Weinberg equilibrium for ABCG2 and FABP3 loci, was found at Hardy-Weinberg equilibrium for AA-NAT locus.

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