Early Markers of Mesenteric Artery Ischemia in Rats

Acute mesenteric ischemia (AMI) is a disease with high mortality (60%-80%). Although it has different etiological causes, the common outcomes are intestinal necrosis and gangrene. The survival rate is reported to be 30-40% on average. Therefore, there is a need for biomarkers to assist the diagnosis. In this study, the early diagnostic values of procalcitonin, lactate, amylase and ferritin levels in AMI were investigated. The rats were divided into two groups:: Control group (Group 1, n=8) consisted of rats which underwent laparotomy only, Study group (Group 2, n=8) consisted of rats who underwent ligation of the superior mesenteric artery (SMA) from the aortic outflow tract to create an experimental ischemia model. Blood samples were taken from both groups at hours 0, 2, 6, 12, and 24, and procalcitonin, lactate, amylase and ferritin levels were compared. Lactate and amylase levels increased in blood in the study group, starting at the 6h after SMA ligation until 24h. Amylase and lactate levels can be used early diagnostic biomarkers in AMI. Increase in procalcitonin level was only significant at the 6h. Ferritin levels did not change in the first 24h and hence can not be considered as an acute phase reactant.

Ratlarda Oluşturulan Mezenterik Arter İskemisinin Erken Belirteçleri

Akut mezenterik iskemi (AMI) potansiyel olarak ölümcül bir hastalıktır (%60-80). Farklı etiyolojik nedenleri olmasına rağmen, mezenterik iskeminin ortak sonuçları bağırsak nekrozu ve gangrendir. Hayatta kalma oranı ortalama %30-40’tır. Bu nedenle tanıda rehberlik edecek biyobelirteçlere ihtiyaç vardır. Bu çalışmada AMI’deki prokalsitonin, laktat, amilaz, ferritin düzeylerinin erken tanısal değerleri araştırıldı. Sıçanlar sadece laporotomy yapılan kontrol grubu (Grup 1, n=8) ve aortik çıkış yolundan superior mezenterik arterin bağlanması ile deneysel bir iskemi modeli oluşturulan deneysel grup (Grup 2, n=8) olarak iki gruba ayrıldı. Sıçanlardan 0., 2., 6., 12. ve 24. saatte alınan kan örneklerindeki PCT, Laktat, Amilaz, Ferritin seviyeleri iki grup arasında karşılaştırıldı. Laktat ve Amilaz’ın kan düzeyleri SMA (Superior Mezenterik Arter) oklüzyonun 6. saatinden başlayarak 24. saate kadar artış gösterdi. Amilaz ve laktat, AMI’de erken tanısal belirteçler olarak kullanılabilir. Prokalsitonin artışı sadece 6. saatte anlamlıydı. Ferritin ise ilk 24 içinde hiç artış göstermediğinden AMI için erken faz belirteci olarak kabul edilmedii.

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