Oğuz İDİZ, Yeliz Emine ERSOY, Ramazan UÇAK, Ebru KANIMDAN, Emrah YÜCESAN, Beyza GONCU, Burcu ÖZDEMİR, Erhan AYSAN

Bir Hayvan Modeli Olarak: Koyunlarda Intravenöz Paratiroid Hücre Zenonaklinin Etkisi

Paratiroid hücre nakli, kalıcı hipoparatiroidizmin tedavisinde etkili bir yaklaşımdır. İntramüsküler ve intraperitoneal yollar daha önce yapılmış, ancak intravenöz uygulama daha önce yapılmamıştır. Amacımız, paratiroid hücreleri için uygun yerleşim bölgesini belirlemekti, bu amaçla paratiroid hücrelerini intravenöz olarak uyguladık ve sonuçları gözlemledik. Bu çalışma dokuz koyun üzerinde gerçekleştirildi. Verilen maddeye göre koyunlar iki gruba ayrıldı; yedisi paratiroid hücresi enjekte edilen grup, ikisi iztonik tuz çözeltisi enjekte edilen kontrol grubudur. Paratiroid hücreleri kronik böbrek yetmezliği olan bir hastadan elde edildi ve hücre izolasyonu sonrası intravenöz olarak koyunlara enjekte edildi. Sırasıyla; 20x106 hücre iki hayvana, 50x106 hücre iki hayvana, 100x106 hücre iki hayvana ve 200x106 hücre bir hayvana hayvana uygulandı ve enjeksiyon sonrası immünsupresyon uygulanmadı. Üç ay süresince hayvanların fiziksel kondisyonları ve kan biyokimyasal testleri gözlemlendi. Sakrifikasyon sonrası, karaciğerler ve böbrekler histopatolojik olarak incelendi. 20x106 ve 50x106 hücre nakledilen gruplarda serum PTH seviyeleri ilk yedi gün yükseldi ancak diğer gruplarda sabit kaldı. Böbreklerin ve karaciğerlerin histopatolojik incelemelerinde, infüze edilen hücre sayısıyla ilişkili fibrozis saptandı, ancak biyokimyasal fonksiyonlarda herhangi bir farklılık tespit edilmedi. İntravenöz paratiroid hücre naklinin, immünsupresyonsuz uygulanacak etkili ve kullanışlı bir teknik olduğu değerlendirildi. Bununla birlikte, klinik amaçla kullanım öncesi ilerleyen dönemde daha kabul edilebilir sonuçları olan uzun süreli çalışmalara ihtiyaç vardır.

The Effect of Intravenously Paratyroid Cell Xenotransplantation in Sheep: As an Animal Model

Parathyroid cell transplantation is an effective approach for the treatment of permanent hypoparathyroidism. Intramuscular and intraperitoneal routes were performed previously but intravenous adminstration has not been conducted previous studies. Our aim is to determine proper homing site for parathyroid cells, therefore we administered parathyroid cells intravenously and observed results. This study is carried out on nine sheep. According to the given substance the sheep were divided into two groups; seven were parathyroid cell injected group and two were isotonic saline solution injected control group. Parathyroid cells were obtained from a patient with chronic kidney failure and were injected intravenously into sheep after cell isolation.: 20x106 cells for two animals, 50x106 cells for two animals, 100x106 cells for two animals, and 200x106 cells for one animal respectively, with no post injection immunosuppresive therapy. Immunosuppresion was not administered. Physical conditions of animals and blood biochemical tests were observed for three months. After sacrifications, kidneys and livers were evaluated histopathologically. In 20x106and 50x106 cell transplanted groups serum PTH levels increased in the first seven days but in the other groups remained stable. Histopathological evaluations of kidneys and livers revealed fibrosis related to the number of infused cells, however biochemical functional differentiations were not detected. Intravenous parathyroid cell transplantation is considered as an effective and useful technique to perform without immunosuppression. However, further and long term studies need to have more acceptable results in future for clinical purpose.

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