Bahar ARGUN KARSLI, Kemal KARABAĞ, Taki KARSLI, Eymen DEMİR, Hüseyin Göktuğ FİDAN, Mehmet ASLAN, Sedat AKTAN, Serdar KAMANLI, Emine ŞAHİN SEMERCİ, Murat Soner BALCIOĞLU

Beş Farklı Beyaz Yumurtacı Saf Hatta Yumurta Verimi ve Kalitesi İle İlişkili Bazı Aday Genlerdeki Polimorfizmler

Mevcut çalışma Ankara Tavukçuluk Araştırma Enstitüsü tarafından yetiştirilen beyaz yumurtacı saf tavuk hatlarında (Black, Brown, Blue, Maroon ve D-229) daha önce yumurta verimi ve kalitesiyle ilişkili olduğu bildirilen aday genlerdeki polimorfizmlerin PCR-RFLP yöntemiyle belirlenmesini amaçlayan ilk kapsamlı çalışmadır. Toplam 11 gen bölgesi 192 tane tavuk için çoğaltılmış ve genotiplerin belirlenmesi için özgün restriksiyon endonükleazlar ile kesilmiştir. Bütün tavuk hatları VIPR-1/HhaI and VIP-501/VspI gen bölgeleri için monorfik olmasına rağmen geriye kalan dokuz gen bölgesinde polimorfizm belirlenmiştir. Bu çalışma Ankara Tavukçuluk Araştırma Enstitüsü’nde yetiştirilen beyaz yumurtacı saf hatlarda (D-229 hariç) yumurta kalitesini iyileştirmek için gelecekte yapılacak MAS çalışmalarında GHR-intron 2/HindIII and GHR-intron 5/NspI polimorfizmlerinin kullanılabileceğini ortaya çıkarmıştır. Ayrıca, yumurta verimini arttırmak için DRD2 ve MR1C genlerinin çalışılan bütün tavuk hatlarında, VIP-I2/HinfI polimorfizminin sadece Maroon hattında, VIPR-2/Ta qI polimorfizminin ise Brown ve D-229 hatlarında MAS çalışmalarında kullanılabileceği belirlenmiştir.

Polymorphisms in Some Candidate Genes Associated with Egg Yield and Quality in Five Different White Layer Pure Lines

This is the first comprehensive study aimed to determine polymorphisms in candidate genes (OCX32-exon4, OCX32-exon2, GHR-intron 2, GHR-intron 5, DRD1, DRD2, VIP-501, VIP-I2, VIPR-1, VIPR-2 and MR1C) reported to associated with egg yield and quality in white layer pure chicken lines (Black, Brown, Blue, Maroon and D-229) raised by Ankara Poultry Research Institute by using PCR-RFLP technique. A total of 11 gene regions were amplificated for 192 chickens and were then digested with specific restriction endonucleases to determine the genotypes. Although all chicken lines were monomorphic for VIPR-1/HhaI and VIP-501/VspI gene region, polymorphisms were detected in the rest of nine gene regions. The present study revealed that GHR-intron 2/HindIII and GHR-intron 5/NspI polymorphsims can be integrated with MAS studies to increase egg quality in white layer chicken lines (D-229 excluded) raised in Ankara Poultry Research Institute in the future. In addition, in order to increase egg yield DRD2 and MR1C gene may be used in MAS for all studied chicken lines, while VIP-I2/HinfI polymorphism can be used for Maroon line and VIPR-2/Ta qI polymorphism for Brown and D-229.

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