Muhamed KATICA, Emina SPAHIĆ, Anes JOGUNČIĆ, Aida KATICA, Sabaheta HASIĆ

Antemortem ve Postmortem Analizlerde Sıcaklığın ve Sıcaklığa Maruz Kalma Süresinin Eritrositlerin Morfolojik Özellikleri Üzerine Etkisi: Wistar Ratlarda Deneysel Çalışma

Kırmızı kan hücresi morfolojisindeki değişiklikler çeşitli uyaranlarla indüklenebilir. Farklı sıcaklık yoğunluklarının (37°C, 41°C ve 44°C) ve maruz kalma süresinin (20 dakika ve ölüm zamanına kadar) eritrosit morfolojisi üzerindeki etkilerini araştırmak için ısı stresi uygulanan bir rat modelinde kullanıldı. Otuz beş Wistar sıçanı üç gruba ayrıldı: kontrol grubu olarak 37°C, deneme grubu olarak 41°C ve 44°C. Deney grupları antemortem 20 dakikalık maruziyet süresi ve postmortem gruplar ölüme kadar maruziyet süresi olarak sınıflandırıldı. Anestezi uygulanan sıçanlar su banyosu kullanılarak önceden ısıtılmış suya maruz bırakıldı. Abdominal aorttan alınan kan örneklerine May-Grünwald-Giemsa boyama tekniği uygulandı. Poikilositleri saptamak için ışık mikroskobu kullanıldı (Motic Tip 102M). Antemortem gruplarında hedef hücreler ve anülositler predominant iken, postmortem gruplarda, 41°C ve 44°C’de sırasıyla anülositler ve sferositler şeklindeydi. Antemortem ve postmortem gruplar arasında 41°C ve 44°C’deki poikilosit sayısında fark bulunmadı. Sferosit sayısı postmortem grubunda, antemortem 41°C grubuna göre (P=0.001) ve dakriyositler sferositlere göre postmortem 44°C grubunda (sırasıyla P=0.002, P=0.017) daha yüksekti. Poikilositozun, sıcaklık yoğunluğu ve maruz kalma süresi ile ilişkili olduğu belirlendi. Postmortem gruplarda en sık sferositoz ve anülositoz görülürken, antemortem gruplarda hedef hücreler ve anülositler görülmekteydi. Ölümcül bir sonucu takiben 41°C’de sferositler ve 44°C’de sferositli dakriyositler, kendilerine karşılık gelen antemortem gruplarından önemli ölçüde daha fazlaydı.

Impact of Temperature and the Length of Exposure on Morphological Characteristics of Erythrocytes in Antemortem and Postmortem Analysis: Experimental Study on Wistar Rats

Changes in red blood cell morphology can be induced by various stimuli. In order to investigate the effects of different temperature intensities (37°C, 41°C, and 44°C) and exposure time (20 min and until the time of death) on erythrocyte morphology, heat stress was used on a rat model. Thirty-five Wistar rats were divided into three groups: 37°C as control group, 41°C and 44°C as trial groups. The trial groups were classified into antemortem the exposure time of 20 min and postmortem groups exposure time until fatal outcome. The anaesthetised rats were exposed to preheated water using the water bath. May-Grünwald-Giemsa colouring technique was applied on blood samples taken from the abdominal aorta. The light microscopy was performed (Motic Type 102M) to detect poikilocytes. Target cells and anulocytes were predominant in antemortem groups, while anulocytes and spherocytes in postmortem groups 41°C and 44°C, respectively. No difference in poikilocyte number was found between antemortem and postmortem groups 41°C and 44°C. Spherocyte number was significantly higher in postmortem than antemortem group 41°C (P=0.001) and dacryocytes with spherocytes in postmortem group 44°C (P=0.002, P=0.017, respectively). Poikilocytosis is associated with the exposure length and temperature intensity. Spherocytosis and anulocytosis are the most frequent in postmortem groups while target cells and anulocytes in antemortem groups. Following a fatal outcome, spherocytes at 41°C and dacryocytes with spherocytes at 44°C were significantly more than in corresponding antemortem groups.

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