Siriwade CHOMDEJ, Chakkapong KUENSAEN, Waranee PRADİT, Korakot NGANVONGPAINT

Detection of DNA markers in dogs with patellar luxation by high annealing temperature - random amplified polymorphic DNA analysis

Patellar luksasyon küçük ırk köpeklerde en yaygın gözlenen bozukluklardan birisidir. Sonraki nesillere aktarılmak suretiyle köpeklerde süregelen bir problem olabilir. Ancak tespit edilmesi durumunda etkilenmiş köpekler üretim amaçlı kullanılmaz ve böylece görülme sıklığazaltılabilir. Bu çalışmada, patellar luksasyon ile doğan köpeklerin tespitinde kullanılmak amacıyla bir DNA markır bulmak amaçlanmıştırHigh annealing temperaturerandom amplified polymorphic DNA (HAT-RAPD) tekniği ile 39 köpeğin (16 normal, 23 patellar luksasyonlukan örnekleri kullanıldı. Üç aday parçacık (fragment) sekanse edildi ve BLAST algoritması kullanılarak Den Bank köpek genomukarşılaştırıldığında bunların üç farklı kromozomdan (10, 36 ve X) bölümler olduğu tespit edildi. Asosiasyon analizi chi-kare testi kullanılarak yapıldı. Sonuçlar 36. kromozomdaki parçacığın (OPB05 primeri ile üretilen) potansiyel olarak iki grup köpek ile ilgili olduğunu ortaya koydu (P=0.042). Bu çalışma köpek patellar luksasyon ile ilgili bir gene ilişkin ilk çalışma olup gelecek araştırmalara ihtiyaç vardır.

Patella Çıkığı Olan Köpeklerde DNA Belirteçlerinin Yüksek Bağlanma Sıcaklıklı RAPD Analizi ile Belirlenmesi

Patellar luxation is one of the orthopaedic disorders found mostly in small-breed dogs. It can be inherited by the next generation, causing continuing problems in the dogs health. However, if it can be detected, the affected dogs will not be selected for breeding, and hence the incidence will be less. In this study, the objective was to find a DNA marker representing dogs with patellar luxation which can be detected when first born. High annealing temperature-random amplified polymorphic DNA (HAT-RAPD) technique was used o amplify 39 dog blood samples (16 unaffected and 23 affected). It was also used to develop the polymorphic fragments capabledistinguishing patellar-luxation-affected dogs from those unaffected. Three candidate fragments were sequenced and found to be parts rom three different chromosomes (10, 36 and X) after comparison with the GenBank dog genome database using the BLAST algorithm. Association analysis was performed using a chi-square test. The results showed that the fragment (generated by the OPB05 primer) from chromosome 36 was potentially related to the two groups of dogs, with a P value of 0.042. This is the first finding of a gene which related o canine patellar luxation, and merits further investigation.

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