OSMAN SELÇUK ALDEMİR, Feyzullah GÜÇLÜ

Diagnosis of Sarcocystis species in cattle in Konya Region

Bu araştırma Konya bölgesi sığırlarında Sarcocystis türlerinin prevalansının tespiti amacıyla tiripsin tekniği kullanılarak gerçekleştirilmiştir. Sığırların kalp, diyafram, ve özefaguslarında %92 oranında mikroskobik kistlere rastlanmış olup, makroskobik kistler tespit edilememiştir. Kist duvarının yapısına göre üç tip mikroskobik kist bulunmuş olup, S. bovicanis74 (%74), S. bovifelis 15 (%15) ve S. bovihominis 3 (%3) oranlarında tespit edilmiştir. Miks olarak ise S. bovicanis + S. bovihominis7 (%7), S. bovicanis + S. bovifelis3 (%3), S. bovihominis + S. bovifelis 3 (%3) ve S. bovicanis + S. bovihominis + S. bovifelis 20 (%20) oranlarında teşhis edilmiştir. Sonuç olarak; Konya bölgesi sığırlarında Sarcocystis spp'ye en fazla özefaguslarda rastlanıldığı, en yaygın türün S. bovicanis olduğu tespit edilmiştir.

Anahtar Kelimeler:

Sarcocystis, sığır, teşhis, hastalık yaygınlığı, metodoloji, tripsin

Konya yöresi sığırlarında Sarcocystis türlerinin teşhisi

: In this study, the trypsin trypsin tech-nique was used to investigate the presence and prevalence of Sarcocystis spp. in the cattle in Konya province. Trypsin technique was able to detect Sarcocystis ssp. in 92 (92%) of 100 cattle (samples of heart, diaphragma and oesophagus) in which macroscopically cysts were not observed. Corresponding values for the Sarcocystis species were as follows: single infections 74 (74 %) S. bovicanis, 15 (15 %) for S. bovifelis and 3 (3 %) S. bovihominis; in mixed infections 7 (7 %) S. bovicanis + S. bovihominis, 3 (3 %) S. bovicanis + S. bovifelis,3 (3 %) S. bovihominis + S. bovifelis and 20 (20 %) S. bovicanis + S. bovihominis + S. bovifelis respectively. In conclusion, microscopically cysts observed the highest number in eosophaugs among samples examined. In this study, Sarcocystis spp. are very common and S. bovicanis is the dominant species in cattle in the Konya region.

Keywords:

Sarcocystis, cattle, diagnosis, disease prevalence, methodology, trypsin,

___

1.Özer E: An experimental investigation on life cycle and pathogenicity of Sarcocys capracanis. PhD Thesis. FÜ Sağ Bil Enst, Elazığ, 1983.
2.Sevinç F, Altınöz F, Uslu U, Aldemir OS: The prevalence of Sarcocystis species in Sheep Slaughtered at Konet Abattoir in Konya. Vet Bil Derg, 16(2): 75-79,2000.
3.Tenter AM: Current research on Sarcocystis species of domestic animals. Int JParasitol, 25(11): 1311-1330, 1995.
4.Dufoey JP, Fayer R: Sarcocystosis- present and future. J Vet Parasitol, 1: 1-6, 1987.
5.Aldemir OS, Dik B: Koyunlardaki Sarcocystis Türlerinin RAPD-PCR ile Teşhisi. TParazitol Derg; 27 (4),2004.
6.Böttner A, Charleston AG, Pomray WE, Rommel M: The prevalence and identify of Sarcocystis in Beef cattle in New Zelland. Vet Parasitol, 24: 157-168,1987.
7.Cerna Z, Merhautova V: Sarcocystis in cattle and sheep at Prague abatoir. Folia Parasitol, 28: 125-129, 1981.
8.Dubey JP, Speer CA, Fayer R: "Sarcocystosis of Animals and Man". CRC Press, Florida 1989.
9.Daugschies A, Rommel M, Hoppen HO: Prostanoids during acute Sarcocystiosis in growing pigs. Parasitol Res, 76: 115- 118,1989.
10.Heydorn A, Gestrich R, Mehlorn M, Rommel M: Proposol for a new nomanculature of the Sarcosporidia. Z Parazitenkd, 48: 73-82, 1975.
11.Kudi AC, Aganga AO, Ogbogu VC, Umoh JU: Prevalence of Sarcocystis species in sheep and goats. Rew Elev Med Vet Pays Ttrop, AA: 59-60,1991.
12.Leek RG, Fayer R: Sheep experimentally infected with Sarcocystis from dogs. II. Abortion and disease in ewes. Cornell Vet, 68: 108-123, 1978.
13.MacPherson JM, Gajadhar AA: Specific amplification of Sarcocystis cruzi DNA using a RAPD. Vet Parasitol, 55(4): 267-77,1994.
14.Munday BL: The prevalence of Sarcosporidiosis in Australian meat animals. Aust VetJ, 51: 478-480, 1975.
15.O'donoghue PJ, Ford GE: The prevalence and intensity of Sarcocystis spp infections in sheep. Aust Vet J, 63: 273-278, 1986.
16.Penner GA, Bush A, Wise R, Scoles G, Molnar SJ, Fedak G: Reproducibility of RAPD analysis among laboratories, PCR Methods Appl, 2(4): 341-345, 1993.
17.Ranki M: Phylogenetic analysis of coccidia based on 18S rDNA sequence comparison indicates that Isospora is most closely related to Toxoplasma and Neospora. J Eukaryot Microbiol, 45(2): 184-188, 1991.
18.Özer E: Investigation on Sarcocystis species and Their incidences in Cattle and Water Bufalo. Doğa Tr Vet and Anim Sci, 12: 130-139. 1988.
19.Okur H, Kandemir O, Şahin İ: An investigation on Sarcocystis species in the cattle and the sheep in Bayburt. T Parazitol Derg, 19: 113-118,1995.
20.Sayın F, Özer E: Doğu Anadolu'da keçilerde Sarcosporidiosis'in yayılışı üzerine araştırmalar. AÜ Vet Fak Derg, 31(2): 316-323,1984.