SENA ÇENESİZ, A. Kadir DEVRİM, NECATİ KAYA, AYLA ÖZCAN, Mahmut SÖZMEN

Azoksimetanla kolonik anormal kript oluşturulmuş farelerde vitamin C' nin aspartat aminotransferaz (AST) ve alanın aminotransferaz (ALT) aktiviteleri üzerine etkisi

Bu çalışmada azoksimetanla kolonik anormal kript formasyonu oluşturulan farelerde vitamin C'nin serum ve karaciğer dokusundaki AST ve ALT aktiviteleri üzerine etkisinin araştırılması amaçlanmıştır. Materyal olarak ortalama 31.49 g ağırlıkta, 30 adet 12 haftalık Swiss Albİno türü fare kullanıldı. Fareler uç gruba ayrıldı. 1. Grup kontrol, 2. Grup azoksimetan, 3. Grup ise azoksimetan+vitamin C verilen grubu oluşturdu. Tüm gruplara deneme boyunca kuru pelet yem ve su ad libitum olarak verildi. İkinci ve 3 Grup hayvanlara haftada 2 gün 7 hafta boyunca 5 mg/kg azoksimetan, 3. Gruba ise. 500 mg/kg vitamin C her gün derialtına enjekte edildi. Enjeksiyonların ardından 6 haftalık bekleme süresinin sonunda hayvanlardan kan ve karaciğer doku örnekleri alındı. Yapılan analizler sonucunda kontrol grubu ile karşılaştırıldığında serum AST aktivitesinde azoksimetan grubunda artış (p

Anahtar Kelimeler:

azoksimetan, istatistiksel analizler, neoplazma, fareler, karaciğer fonksiyonları, karaciğer, enzim aktivitesi, kolon, aspartat aminotransferaz, askorbik asit, alanin aminotransferaz

Effect of vitamin C supplementation on the aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities of mice which was ınduced colonic aberrant crypt foci formation by azoxymethane

In this study we investigated the effect of vitamin C on AST and ALT activities in the sera and liver tissues of the mice which have colonic abnormal crypt formation formed by azoxymethane. 30 Swiss Albino mice which are on avarage 12 weeks old and 31.49 g weight were used in this study. The mice were seperated into 3 groups. First Group was the control group. We applied azoxymethane to the Second Group and azoxymethane+vitamin C to the Third Group. Mice were fed ad libitum by dry grain feed and drunk water during the experiment period. During 7 weeks and two times a week, 5 mg/kg azoxymethane were injected SC to the Second and Third Groups and 500 mg/kg vitamin C were injected SC to the Third Group. At the end of the experiment we got the sera and liver tissue samples from all of the groups. In the sera AST activities, we observed increases in the Second Group when compared with First Group (p<0.001). Although, statistically we did not observed any alterations in the Third Group. Additionally in the sera ALT activities, we did not observed any alterations in the Second and Third Groups when compared with First Group. AST activities in the liver tissue did not show any alterations statistically. On the contrary, in ALT activities in the liver tissues we observed an increase in the Third Group (p<0.05) and a major increase in the Second gGroup (p<0.001). Consequently, it is observed that application of vitamin C which is used as an anticancerogen, in the abnormal crypt formation which is the development period of colon cancer has a positive effect on AST and ALT activities which are important markers of the hepatic damage.

Keywords:

azoxymethane, statistical analysis, neoplasms, mice, liver function, liver, enzyme activity, colon, aspartate aminotransferase, ascorbic acid, alanine aminotransferase,

PDF

___

1.Brady LJ, Gallaher DD, Busta FF: The role of probiotic cul tures in the prevention of colon cancer. / Nutr, 130:410-414, 2000.
2.Jacobs EJ, Conell CJ, Patel AV, Chao A, Rodriguez C, Sey mour J, McCulloogh ML, Calle EE, Thun MJ: Vitamin C and vitamin E supplement use and colorectal cancer mortalit- yin a large American cancer society cohort. Cancer Epidemiology, Biomarkers & Prevention, 10:17-23, 2001.
3.Langman M, Boyle P: Chemoprevention of cancer. Gut, 43:578-585,1998.
4.Kuchmar JF, Moss DW: Fundamentals of Clinical Chemistry. Enzymes (Nobert W.T.) WB Saunders Company. Philadelphia, 562-698,1982.
5.Cornelius CE: Liver function. In, Kaneko JJ (Ed): Clinical Biochemistry of Domestic Animals. 3rd ed. New York, Lon don, Academic Press, 230-242, 1980.
6.Whitby LG, Percy-Robb IW, Smith AT: Enzyme test in diagnosis. Blackwell Sci Pub, 138-69,1984. -: ' .
7.Kuchel PW, Ralston GB: Schaum's outline of theory and problems of biochemistry. McGraw-Hill Inc, 411-49,1988.
8.Chung H, Wu D, Han SN, Gay R, Goldin B, Bronson RE, Mason JB, Smith DE, Meydani SN: Vitamin E supplemen tation does not alter azoxymethane-mduced colonic aberrant crypt foci formation in young or old mice. J Nutr, 133:528- 532, 2003.
9.Koul A, Mishra A, Nehru B: Modulation of oxidative stress by ascorbic acid and/or a-tocopherol. J Nutr&Envir Med, 10, 233-238, 2000.
10 Richterich R: Clinical Chemistry. Theory and Practice. S. Kargel, Basel (Switzerland). Academy Press, 321- 431. 1969.
11 Byers T, Nestle M, McTiernan A, Doyle C, Currie-Williams A, Gangster T, Thun M: CA Cancer J Clin, 52:92-119, 2002.
12 Cameron E, Pauling L: Supplemental ascorbate in the sup portive treatment of cancer: prolongation of survival times in ter minal human cancer. Proc Natl Acad Sci, 73: 3685-3689: 1976.
13 Kakizoe T: Chemoprevention of cancer-focusing on clinical trials. Jpn J Clin Oncol, 33(9) 421-442, 2003.