Davut BAYRAM, Bilal AKYÜZ, Korhan ARSLAN, Fadime ÖZDEMİR, ESMA GAMZE AKSEL, Mehmet Ulaş ÇINAR

DGAT1, CAST and IGF-I Gene Polymorphisms in Akkaraman Lambs and Their Effects on Live Weights up to Weaning Age

Early live weight in sheep is important for lamb survival and average weight gain until slaughter. The aim of the present study was to investigate association between CAST-MspI, DGAT1-AluI and IGF-1-Bsp143II polymorphisms and early live weights between birth and weaning age in Akkaraman lambs. A total of 374 lambs were genotyped for CAST-MspI, DGAT1-AluI and IGF-1-Bsp143II polymorphisms by the polymerase chain reaction and restriction length polymorphism (PCR-RFLP) method. The results of PCR-RFLP analysis showed that the SNPs had three genotypes of CAST-MspI polymorphism, two genotypes of DGAT1-AluI polymorphism and one genotype of IGF-1-Bsp143II polymorphism of these, CAST-MspI-MM, DGAT1-AluI-CC and IGF-1-Bsp143II-BB were the predominant genotypes in the Akkaraman sheep breed. The result of Chi-square analysis indicated that the Akkaraman sheep breed was in Hardy-Weinberg equilibrium for the investigated polymorphic genes. At the DGAT1 locus, the CT genotype showed significantly heavier birth weight (P=0.044) compared to CC genotype. CAST gene did not show any association for the investigated traits. The results of this study demonstrate that the CT genotype had a positive effect on birth weight in Akkaraman sheep. We concluded that further investigations are needed in DGAT1-AluI polymorphism and live weight at different ages in sheep.

Akkaraman Kuzularında DGAT1, CAST ve IGF-I Gen Polimorfizmleri ve Sütten Kesim Yaşına Kadarki Canlı Ağırlık Artışı Üzerine Etkileri

Bu çalışmada, Akkaraman ırkı kuzularda, CAST-MspI, DGAT1-AluI ve IGF-1-Bsp143II polimorfizmleri ile bu polimorfizmler ve bazı canlı ağırlıklar arasındaki ilişkilerin araştırılması amaçlanmıştır. PCR-RFLP yöntemi ile toplam 374 kuzu CAST-MspI, DGAT1-AluI ve IGF-1-Bsp143II polimorfizmleri yönünden genotiplendirilmiştir. Akkaraman ırkı koyunların, CAST-MspI ve DGAT1-AluI polimorfizmleri yönünden polimorfik iken IGF-1-Bsp143II polimorfizmi yönünden monomorfik oldukları görülmüştür. Akkaraman ırkı koyunlarda CAST-MspI-MM, DGAT1-AluI-CC ve IGF-1-Bsp143II-BB genotiplerinin en yaygın genotipler olduğu görülmüştür. Ki-kare test sonuçlarına göre Akkaraman ırkı koyunların CASTMspI ve DGAT1-AluI polimofizmleri yönünden Hardy-Weinberg (HW) dengesinde oldukları görülmüştür. DGAT1 lokusu yönünden TC genotipli kuzular, diğer genotiplilerle karşılaştırıldığında bu genotipteki kuzuların daha yüksek doğum ağırlığına (P

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