Jalil HEIDARI, Jamal SEIFDAVATI, Hossein MOHEBODINI, Reza SEYED SHARIFI, Hossein ABDI BENEMAR

Effect of Nano Zinc Oxide on Post-Thaw Variables and Oxidative Status of Moghani Ram Semen

The damages of freezing technique of sperm including motility, survival and membrane disorders involves the formation of ice crystals, oxidative stress, osmotic changes and the re-organization of lipid-protein in the cell membrane. Zinc plays basic role in membrane stability and physical properties of the attached fibers, sperm tail morphology and sperm motility. The aim of this study was to evaluate effect of Nano zinc oxide (Nano-znO) on post-thaw variables and oxidative status of Moghani ram semen. In order to collect semen of four Moghani rams, 3-4 years old with an average weight of 80-90 kg were used to twice a weekly. Sperm samples (in five replicates) were frozen in five groups: control group, 0.01, 0.1, 0.5 and 1 mg/mL of Nano-znO. The hypo-osmotic swelling test (HOST) was used to evaluate the integrity of the plasma membrane of the sperm. The results showed that the average of integrity of the plasma membrane of sperm and MDA production in the medium containing 0.1 mg/mL of Nano-znO was with the highest and lowest percentages, respectively (P

Nano Çinko Oksitin Moghani Koç Semeninin Çözdürme Sonrası Değişkenler ve Oksidatif Durumuna Etkisi

Spermin dondurulma tekniğine bağlı olarak şekillenen mortalite, canlılık ve membran bozuklukları hasarları buz kristallerinin oluşmasını, oksidatif stresi, ozmotik değişiklikleri ve hücre zarında lipit-protein reorganizasyonunu içermektedir. Çinko membran stabilitesi, ataçlanmış fiberlerin fiziksel özellikleri, sperm kuyruk morfolojisi ve sperm motilitesi üzerinde önemli rol oynar. Bu çalışmanın amacı nano çinko oksitin (Nano-ZnO) Moghani koç semeninin çözdürme sonrası değişkenler ve oksidatif durumuna etkisini araştırmaktır. Ortalama ağırlıkları 80-90 kg olan 3-4 yaşlı dört Moghani koçundan haftada iki kere semen toplandı. Sperm örnekleri (beş tekrar olarak) kontrol, 0.01, 0.1, 0.5 ve 1 mg/mL Nano-ZnO ile dondurulan gruplara ayrıldı. Spermlerin plazma membran bütünlüklerini değerlendirmek amacıyla Hipo ozmotik şişme testi (HOST) kullanıldı. 0.1 mg/mL Nano-ZnO ile dondurulan spermanın sperm plazma membran bütünlüğü ortalaması en yüksek iken MDA üretimi ortalaması en düşük olarak belirlendi (P

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