MEHMET ERAY ALÇIĞIR, TUNCER KUTLU, Ekrem Çağatay ÇOLAKOĞLU, Ceyda DİNÇ

Desmoplastic Small Round-Cell Tumor in a Dog

A 13-year-old, neutered female Husky dog was brought to the clinic with the complaints of anorexia, vomiting, abdominal distension and respiratory distress. It was suddenly died during intervention. At the necropsy, a mass was seen in the abdominal cavity. The mass had 16x15x9 cm in diameters. Histological examination revealed clusters of cells with slightly eosinophilic and scanty cytoplasm, small round hyperchromatic nuclei, and inconspicuous nucleoli encompassed by hypocellular extensive desmoplastic connective tissue stroma comprising few spindle-shaped connective tissue cells. In immunohistochemical examination the cytoplasms of tumour cells were detected to be mildly positive by nestin. The tumour cells were negative for α-SMA (Alpha-Smooth Muscle Actin), vimentin and pancytokeratin, but stromal cells were positive for α-SMA and vimentin. Despite the presence of partially incompatible immunohistochemical findings, the tumor in this case was diagnosed as desmoplastic small round-cell tumor because its aggressiveness, localization, and histopathology was similar to that observed for this tumor in humans. Previously this tumor has not been identified in animals.

Bir Köpekte Küçük Yuvarlak Hücreli Dezmoplastik Tümör

Husky ırkı, 13 yaşlı, kısırlaştırılmış dişi bir köpek, anoreksi, kusma, karın şişliği ve solunum sıkışması şikayetiyle kliniğe getirildi. Hayvana müdahele sırasında aniden öldü. Nekropside karın boşluğunda 16x15x9 cm boyutlarında bir kitleyle karşılaşıldı. Histolojik incelemede hiposelüler, az sayıda mekik şekilli bağ doku hücrelerinden oluşan geniş desmoplastik bağ doku stromasıyla adacıklara ayrılan; hafif eozinofilik dar sitoplazmalı, küçük yuvarlak-oval hiperkromatik çekirdekli, çekirdecikleri fark edilmeyen hücre kümeleri gözlendi. İmmunohistokimyal incelemede tümör hücre sitoplazmalarının nestin yönünden hafif pozitif olduğu saptandı. Tümör hücreleri α-SMA (Alpha-Smooth Muscle Actin), vimentin ve pansitokeratin negatif olup stroma α-SMA ve vimentin pozitifti. İmmunohistokimyasal yönden kısmen farklı bulgular göstermekle beraber gerek lokalizasyonu gerekse histomorfolojik açıdan insanlardakine benzerliklerinden dolayı bu tümöre desmoplastik küçük yuvarlak hücreli tümör teşhisi konmuştur. Daha önce bu tümör hayvanlarda tanımlanmamıştır.

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