Xunliang LI, Pengchong LI, Huijie CHEN, Fandan MENG, Xiaofeng REN, Guangxing LI

The DNA Vaccine Combining the Adjuvant Porcine IL-12 with the Spike Gene of Transmissible Gastroenteritis Virus Enhances the Immune Response in Swine

Transmissible gastroenteritis (TGE), cause by transmissible gastroenteritis virus (TGEV), is an acute digestive and highly infectious disease in piglets. In this study, porcine interleukin-12 immunological adjuvant combined with a DNA vaccine bearing the TGEV-S1 gene was used to immunize piglets, and assessed the immune response of piglets. The results showed that CD4+ and CD8+CD28+ T lymphocytes were significantly increased in immunized piglets compared to control groups. In addition, the observed increase of IFN-γ suggested that T helper cells tended to convert to Th1 cells during immune response. HE stain and indirect immunofluorescence assays indicated obvious differences between the immunized piglets and the controls, suggesting that immunized piglets suffered less pathological changes relative to piglets in the control groups. Moreover, the levels of anti-TGEV and neutralizing antibodies in serum also indicated an effective immune response in the immunized piglets by which the pathogen was rapidly controlled. Altogether, our results suggest that the combination of pVAX1-(pIL-12) and pVAX1-(TGEV-S1) in a vaccine is capable of producing a significantly enhanced immune response in piglets.

Adjuvant Domuz IL-12 ile Kombine Transmissible Gastroenteritis Virüs Spike Geni Taşıyan DNA Aşı Domuzlarda Bağışıklık Yanıtı İyileştirir

Transmissible gastroenteritis virüs (TGEV)’un neden olduğu Transmissible gastroenteritis (TGE) akut sindirim sistemi hastalığı olup domuzlarda oldukça enfeksiyöz bir hastalıktır. Bu çalışmada, TGEV-S1 geni taşıyan DNA aşısı ile kombine olarak domuz interlökin-12 immunolojik adjuvant domuzları immunize etmek amacıyla kullanıldı ve domuzlarda meydana gelen immun yanıt değerlendirildi. Sonuçlar, kontrol grubu ile karşılaştırıldığında immunize edilen domuzlarda CD4+ ve CD8+CD28+ T lenfositlerin anlamlı derecede arttığını gösterdi. Ayrıca, artan IFN-γ immun yanıt süresince yardımcı T lenfositlerin Th1 hücrelerine dönüştüğüne işaret etmekteydi. HE boyaması ve indirek immunofloresans tekniği immunize edilenler ile kontrol arasında bariz farkların olduğunu gösterdi. Bu durum immunize edilen domuzların kontrol grubundakine oranla daha az patolojik değişikliklere maruz kaldığına işaret etmekteydi. Serumdaki anti-TEGV seviyesi ve nötralize edici antikorların miktarı immunize edilen domuzlarda daha etkili immun yanıtın oluştuğunu ve patojenin hızlı bir şekilde kontrol altına alındığını gösterdi. Elde edilen sonuçlar, pVAX1-(pIL-12) ve pVAX1-(TGEV-S1)’nin aşıda kullanılmasının domuzlarda immun yanıtı anlamlı oranda iyileştireceğini göstermiştir.

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