Faisal SIDDIQUE, Rao Zahid ABBAS, Asif IQBAL, Masood RABBANI, Azhar RAFIQUE, Irshad HUSSAIN, Rais AHMED, M. Shahid MAHMOOD, Alireza LOTFI

Development of Humoral Immune Response to Thermostable Newcastle Disease Vaccine Strain I-2 in Ring-Necked Pheasant (Phasianus colchicus)

Newcastle Disease (ND) was ranked as a List A and trade limited disease by the World Health Organization. It is one of the deadly diseases of domestic and zoo birds especially pheasants that are highly susceptible species to Newcastle disease virus (NDV). This study was designed to determine the humoral immune response (HIR) of thermostable NDV vaccine strain I-2 in same age pheasants. For this purpose, forty-five pheasants of the same age were separated and placed in cages. Thermostable NDV I-2 vaccine was mixed with feed and administered through oral route to the same age pheasants. HIR was detected using haemagglutination inhibition test (HI) and enzyme linked immunosorbent assay (ELISA) on 0, $7^{th}$, $14^{th}$, $21^{th}$ and $28^{th}$ days post-vaccination (DPV). Optimum geometric mean anti-NDV-ELISA (2380) and anti-NDV-HI (Log27.5) antibodies titers were identified on $14^{th}$ DPV. It was concluded that the oral administration of NDV I-2 strain is able to elicit a protective immune response in pheasants. Moreover, the use of this novel vaccine technique at the same age of pheasants overwhelms the attempt to catch these birds for single vaccination.

Halka Boyunlu Sülün’de (Phasianus colchicus) Termostabil Newcastle Hastalığı Aşı Suşu I-2’ye Karşı Humoral İmmun Yanıtın Gelişimi

Newcastle Hastalığı, Dünya Sağlık Örgütü’nün A listesinde yer alan ve ticareti sınırlayan bir hastalıktır. Evcil ve hayvanat bahçesi kuşlarının, özellikle de Newcastle Hastalığı Virüsüne (NDV) oldukça duyarlı türler olan sülünlerin ölümcül hastalıklarından birisidir. Bu çalışma, aynı yaştaki sülünlerde termostabil NDV aşı suşu I-2’ye karşı şekillenen humoral immün yanıtı belirlemek için tasarlanmıştır. Bu amaç doğrultusunda kırk beş sülün ayrılarak kafeslere yerleştirildi. Yemle karıştırılan termostabil NDV I-2 aşısı, sülünlere oral yolla verildi. Humoral immun yanıt, aşılamadan sonraki 0., 7., 14., 21. ve 28. günlerde (DPV) Hemaglütinasyon İnhibisyon (HI) testi ve Enzim İşaretli İmmünosorbent Testi (ELISA) kullanılarak tespit edildi. Optimum geometrik ortalama anti-NDV-ELISA (2380) ve anti-NDV-HI (Log27.5) antikor titreleri aşı uygulamasını takiben 14. günde saptandı. NDV I-2 suşunun oral yolla uygulamasının sülünlerde koruyucu bir immün yanıt oluşturabileceği kanaatine varıldı. Ayrıca, aynı yaştaki sülünlerde kullanılan bu yeni aşı uygulama tekniği, aşılama sırasında bu kuşların tek tek yakalanma girişimlerinin üstesinden de gelmektedir.

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