Lei ZHANG, Song REN, Herong LIAO, Jie SUN

High-throughput Sequencing Analysis of miRNA Expression in Embryonic Chicken Breast Muscle

The embryonic period is a critical period for the development of muscle and adipose tissue in chickens. MicroRNAs (miRNAs) have been shown to play important roles in various biological processes, but little is known about miRNAs in chicken embryos. To investigate functional miRNAs regulating the meat quality of adult chickens, embryonic breast muscle tissues of Cobb broilers (CB) and rose-crowned chickens (RC) were collected and analyzed with high-throughput small RNA deep sequencing. The results showed that 842 known miRNAs and 598 novel miRNAs were identified from the four sequencing libraries, of which 592 were diff erentially expressed (DE) miRNAs (P

Embriyonik Tavuk Göğüs Kasında miRNA Ekspresyonunun Yüksek Verimli Dizi Analizi

Embriyonik dönem, tavuklarda kas ve yağ dokusunun gelişimi için kritik bir dönemdir. MikroRNA (miRNAs)’ların çeşitli biyolojik süreçlerde önemli roller üstlendikleri gösterilmiştir, fakat tavuk embriyolarında miRNA’lar hakkında çok az şey bilinmektedir. Yetişkin tavuklarda et kalitesini düzenleyen işlevsel miRNA’ları araştırmak için Cobb broylerlerinin (CB) ve gül taçlı tavukların (RC) embriyonik göğüs kas dokuları toplandı ve yüksek verimli küçük RNA dizi analizleri gerçekleştirildi. Sonuçlar, 592’si farklı olarak eksprese olan (DE) miRNA’lar (P

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