Aytül KÜRÜM, Hakan KOCAMIŞ, Siyami KARAHAN, Mustafa TÜRK, Yasin ÖZKABADAYI

OVGP1 Expression in BOEC and Oviduct: An Immunohistochemical and Immunocytochemical Study

Oviduct is an important tubular organ fostering critical physiological processes such as transport of gametes and embryos, capacitation, fertilization, early embryo development, and maturation of gametes. The aim of the present study was to evaluate oviduct specific glycoprotein-1 (OVGP1) expression in the oviduct regions at diff erent phases of the sexual cycle and bovine oviduct epithelial cells (BOEC). In the study, oviduct samples collected from 6 cows in estral and luteal phases were used. The oviduct samples were collected from the ampulla, isthmus and fimbria and evaluated through routine histology and immunohistochemical studies for OVGP1. The primary BOEC were obtained from the ampulla region and characterized by cytokeratin expression. The immunohistochemistry assay indicated that OVGP1 is expressed in secretory cells of the bovine oviduct. OVGP1 expression varies by the oviduct regions and phases of the sexual cycle. Changes in OVGP1 expression during the sexual cycle suggestively indicates a hormonal infl uence. Regional diff erence in OVGP1 expression is most likely related to the physiological events that occur in diff erent regions of the oviduct. BOEC isolated from the oviduct of estral and luteal phases also expresses OVGP1. Further studies should focus on possible role of OVGP1 in adaption of BOEC to very tedious condition like cell culture.

BOEC ve Ovidukt’ta OVGP1 Ekspresyonu: İmmunohistokimyasal ve İmmunositokimyasal Çalışm

Ovidukt, gametlerin olgunlaşması, erken dönemde embriyo gelişimi fertilizasyon, kapasitasyon embriyo ve gametlerin taşınması gibi kritik fizyolojik süreçleri destekleyen önemli bir tubuler organdır. Bu çalışmanın amacı, seksüel siklusun farklı evrelerinde oviduktun bölgelerinde ve sığır ovidukt epitel hücrelerinde (BOEC) ovidukta özgü glikoprotein-1 (OVGP1) ekspresyonunu değerlendirmektir. Bu çalışmada östral ve luteal dönemdeki 6’şar inekten alınan ovidukt örnekleri kullanıldı. Oviduktun ampulla, istmus ve fimbriya bölgelerinden alınan örneklerde, OVGP1 immünohistokimyasal çalışmalarla değerlendirildi. BOEC ampulla bölgesinden elde edildi ve sitokeratin ekspresyonu ile karakterize edildi. İmmünohistokimya ile, OVGP1’in sığır oviduktunda özellikle sekretorik hücrelerde bulunduğu gösterildi. OVGP1 ekspresyonu, oviduktun bölgelerine ve seksüel siklusun dönemlerine göre değişmektedir. Seksüel siklus sırasında OVGP1 ekspresyonundaki değişiklikler muhtemel bir hormonal bir etkiyi düşündürür. OVGP1 ekspresyonundaki bölgesel farklılık, büyük olasılıkla yumurta kanalının farklı bölgelerinde meydana gelen fizyolojik olaylarla ilgilidir. Estral ve luteal dönemlerde ovidukttan izole edilen BOEC’te OVGP1’i ekspresse etmektedir. Bundan sonraki çalışmalar, OVGP1’in BOEC’in zorlu hücre kültürü şartlarına, adaptasyonundaki rolüne odaklanmalıdır.

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