Min ZHANG, Yu YE, Qiong WU, Fan-fan ZHANG, Yu-xin TANG, Nannan GUO, Dan LEI, Dong-yan HUANG, De-ping SONG, Hao LI

Çin’de Yaban Domuzunda (Sus scrofa) Astrovirusların Tanımlanması ve Genetik Karakterizasyonu

Domuz astrovirusu (PAstV) dünya çapında ishalli domuzlarda en sıklıkla belirlenen bir virustur. Bu çalışmada, Çin’in Jiangxi eyaletinde yaban domuzunda (Sus scrofa) astrovirusun ilk tespiti ve tüm genom sekans analizi gerçekleştirilmiştir. Astrovirus WBAstV/CH/2015’un tüm genom sekansı amplifiye edildi ve belirlendi. Sekans homoloji analizi, WBAstV/CH/2015’in dünye çapında PAstVları ile %40.8 ile %79.7 arasında benzerliğe sahip olduğunu ve Macaristan’da tespit edilen diğer bir yaban domuzu astrovirus (WBAstV) suşu olan WBAstV-1/2011/HUN ile en yüksek benzerliğe (%79.7) sahip olduğunu gösterdi. Filogenetik analiz WBAstV/CH/2015’in WBAstV-1/2011/HUN ile yakın akraba olduğunu ve PAstV 4 topluluğuna yakın olarak yer aldığını göstermiştir..

Identification and Genetic Characterization of Astrovirus in Wild Boar (Sus scrofa) in China

Porcine astrovirus (PAstV) is a frequently detected virus in pigs suffering from diarrhea worldwide. Here, we report the first identificationand complete genome sequence of astrovirus in wild boar (Sus scrofa) in Jiangxi, China. The complete genome sequence of a representativeastrovirus, WBAstV/CH/2015, was amplified and determined. Sequence homology analysis showed that WBAstV/CH/2015 had 40.8%to 79.7% homology with PAstVs worlwide, and shared the highest homology (79.7%) with another wild boar astrovirus (WBAstV) strainWBAstV-1/2011/HUN from Hungary. Phylogenetic analysis showed that WBAstV/CH/2015 was closely related to WBAstV-1/2011/HUN andlocated in the cluster of PAstV 4.

PDF

___

Dennehy PH, Nelson SM, Spangenberger S, Noel JS, Monroe SS, Glass RI: A prospective case-control study of the role of astrovirus in acute diarrhea among hospitalized young children. J Infect Dis, 184 (1): 10-15, 2001. DOI: 10.1086/321007
Padmanabhan A, Hause BM: Detection and characterization of a novel genotype of porcine astrovirus 4 from nasal swabs from pigs with acute respiratory disease. Arch Virol, 161 (9): 2575-2579, 2016. DOI: 10.1007/s00705-016-2937-1
Shan T, Li L, Simmonds P, Wang C, Moeser A, Delwart E: The fecal virome of pigs on a high-density farm. J Virol, 85 (22): 11697-11708, 2011. DOI: 10.1128/JVI.05217-11
Luo Z, Roi S, Dastor M, Gallice E, Laurin MA, L’Homme Y: Multiple novel and prevalent astroviruses in pigs. Vet Microbiol, 149, 316-323, 2011. DOI: 10.1016/j.vetmic.2010.11.026
Cai Y, Yin W, Zhou Y, Li B, Ai L, Pan M, Guo W: Molecular detection of Porcine astrovirus in Sichuan Province, China. Virol J, 13:6, 2016. DOI: 10.1186/S12985-015-0462-6
Shan T, Li L, Simmonds P, Wang C, Moeser A, Delwart E: The fecal virome of pigs on a high-density farm. J Virol, 85 (22): 11697-11708, 2011. DOI: 10.1128/JVI.05217-11
Xiao CT, Luo Z, Lv SL, Opriessnig T, Li RC, Yu XL: Identification and characterization of multiple porcine astrovirus genotypes in Hunan province, China. Arch Virol, 162 (4): 943-952, 2016. DOI: 10.1007/s00705- 016-3185-0
Song D, Huang D, Peng Q, Huang T, Chen Y, Zhang T, Nie X, He H, Wang P, Liu Q, Tang Y: Molecular characterization and phylogenetic analysis of porcine epidemic diarrhea viruses associated with outbreaks of severe diarrhea in piglets in Jiangxi, China 2013. PLoS One, 10 (3):e0120310, 2015. DOI: 10.1371/journal.pone.0120310
Zhang F, Ye Y, Song D, Guo N, Peng Q, Li A, Zhou X, Chen Y, Zhang M, Huang D, Tang Y: A simple and rapid identification method for newly emerged porcine Deltacoronavirus with loop-mediated isothermal amplification. Biol Res, 50:30, 2015. DOI: 10.1186/s40659-017-0135-6
Li JS, Li MZ, Zheng LS, Liu N, Li DD, Duan ZJ: Identification and genetic characterization of two porcine astroviruses from domestic piglets in China. Arch Virol, 160 (12): 3079-3084, 2015. DOI: 10.1007/ s00705-015-2569-x
Cai Y, Yin W, Zhou Y, Li B, Ai L, Pan M, Guo W: Molecular detection of Porcine astrovirus in Sichuan Province, China. Virol J, 13:6, 2016. DOI: 10.1186/s12985-015-0462-6
Machnowska P, Ellerbroek L, Johne R: Detection and characterization of potentially zoonotic viruses in faeces of pigs at slaughter in Germany. Vet Microbiol, 168 (1): 60-68, 2014. DOI: 10.1016/j.vetmic.2013.10.018
Reuter G, Nemes C, Boros A, Kapusinszky B, Delwart E, Pankovics P: Astrovirus in wild boars (Sus scrofa) in Hungary. Arch Virol 157 (6): 1143- 1147, 2012. DOI: 10.1007/s00705-012-1272-4
Bridger JC: Detection by electron microscopy of caliciviruses, astroviruses and rotavirus-like particles in the faeces of piglets with diarrhoea. Vet Rec, 107 (23): 532-533, 1980.
Shimizu M, Shirai J, Narita M, Yamane T: Cytopathic astrovirus isolated from porcine acute gastroenteritis in an established cell line derived from porcine embryonic kidney. J Clin Microbiol, 28 (2): 201-206, 1990.
Lee S, Jang G, Lee C: Complete genome sequence of a porcine astrovirus from South Korea. Arch Virol, 160 (7): 1819-1821, 2015. DOI: 10.1007/s00705-015-2436-9
Liu N, Wang F, Zhang D: Complete sequence of a novel duck astrovirus. Arch Virol, 159 (10): 2823-2827, 2014. DOI: 10.1007/s00705-014-2141-0
Sajewicz-Krukowska J, Domanska-Blicharz K: Nearly full-length genome sequence of a novel astrovirus isolated from chickens with ‘white chicks’ condition. Arch Virol 161 (9): 2581-2587, 2016. DOI: 10.1007/ s00705-016-2940-6
Brnic D, Jemersic L, Keros T, Prpic J: High prevalence and genetic heterogeneity of porcine astroviruses in domestic pigs. Vet J, 202 (2): 390- 392, 2014. DOI: 10.1016/j.tvjl.2014.08.015
Awe OO, Kang KI, Ibrahim M, Ali A, Elaish M, Saif YM, Lee CW: Agerelated susceptibility of turkeys to enteric viruses. Avian Dis, 59 (2): 207- 212, 2003. DOI: 10.1637/10907-071514-Reg