Xiaofeng CHI, Xuemei DING, Xi PENG, XİAOCONG LI, Jing FANG

Broilerlerde Kitosan Olisakkarit İlavesinin İmmun Organlarda Hücre Siklusu Üzerine Etkileri

Bu çalışmanın amacı Kitosan Oligosakkaritlerin (COS) broiler tavuklarda hücre siklusu ile timus, bursa Fabrisius (BF) ve dalak organ ağırlıklarına etkisini araştırmaktır. Üç yüz altmış adet bir günlük sağlıklı Arbor Acres erkek broiler rastgele olarak kontrol, COS I, COS II ve COS III olmak üzere gruplara ayrıldı ve sırasıyla gruplara 0, 200, 350 ve 500 mg/kg COS altı hafta süresince verildi. İmmun organların orantısal ağırlıkları 42. günde COS II grubunda kontrol grubuna göre daha yüksekti. Kontrol grubu ile karşılaştırıldığında 21. günde COS II ve COS III gruplarında yüzde G2/M faz timositler artmıştı. 42. günde COS II grubunda kontrol grubuna göre daha yüksekti. Kontrol grubu ile karşılaştırıldığında 21. günde COS II ve COS III gruplarında G0/G1 faz hücrelerin yüzdesi azalmış, G2/M faz hücrelerin yüzdesi ile timusun proliferasypn indeksi (PI) ise artmıştı. 21. günde COS II grubunda G0/G1 faz BF hücrelerin yüzdesi daha düşük ve S faz hücrelerin yüzdesi ile BF'nin PI'sı daha yüksekti. 42. günde COS II grubunda G2/M faz splenositlerde ve PI'da artma ile G0/G1 faz splenositlerde azalma görülebilir.

Effects of Chitosan Oligosaccharides Supplementation on the Cell Cycle of Immune Organs in Broilers

The objective of this study was to investigate effects of COS on cell cycle and relative weights of thymus, bursa of fabricius (BF) and spleen in broilers. Three hundred and sixty 1-day-old healthy Arbor Acres male broilers were randomly divided into control, COS I, COS II and COS III groups, which were respectively fed with diets containing 0, 200, 350 and 500 mg/kg COS for six weeks. The results showed that the relative weights of immune organs were higher in the COS group II than those in the control group at 42 d. When compared with the control group, increased percentages of G2/M phase thymocytes in the COS groups II and III at 21 d, and decreased percentage of G0/G1 phase cells, increased percentage of G2/M phase cells and PI (proliferation index) of thymus were observed at 42 d. The percentage of G0/G1 phase BF cells was lower, and the percentage of S phase cells and PI of BF were higher in the COS group II at 21 d. The increase of G2/M phase splenocytes and PI, decrease of G0/G1 phase splenocytes in the COS group II could be seen at 42 d.

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