SEFER ÜSTEBAY, Ömür ÖZTÜRK, ALİ BİLGE, Döndü Ülker ÜSTEBAY, Aysu Hayriye TEZCAN

Yumuşak Doku Yaralanması Sonrası Ozon Tedavisinin Etkileri ve IGF-1 Seviyesiyle Olan İlişkisi: Rat Modelinde Deneysel Bir Çalışma

Ozon tedavisinin yumuşak doku hasarı üzerindeki etkilerini araştırmak ve deneysel bir rat modelinde ozon tedavisinden sonra serum IGF-1 düzeylerinde bir değişikliğin olup olmadığını gözlemlemek amaçlandı. Yirmidört yetişkin Wistar albino 240-350 g ağırlığında erkek rat randomize olarak iki gruba ayrıldı. Her iki gruptaki ratların sol arka ekstremitelerine standartlaştırılmış deneysel yumuşak doku yaralanması oluşturuldu. Grup 1'e intraperitoneal olarak (20 µg/mL) günlük ozon tedavisi uygulandı. Grup 2'ye ise sadece beslenme ve rutin bakım yapıldı. Deneysel travmanın 5 ve 15. günlerinde tüm ratlar vücut ağırlığı, duyusal ve motor fonksiyon açısından değerlendirildi. Kan örnekleri Grup 1 ve Grup 2'de intrakardiak alındı ve serum IGF-1 seviyeleri 15. günde ölçüldü. Yumuşak doku hasarının oluşumundan sonra takip süresi boyunca Grup 2'deki üç rat topikal tetrasiklin tedavisine olumlu cevap veren kutanöz enfeksiyona sahipti. Her iki grupta, 5. ve 15. günlerde duyusal ve motor fonksiyonlarla ilgili benzer sonuçlar görüldü. Grup I'deki serum IGF-1 düzeyi grup 2'e göre anlamlı derecede yüksekti (P=0.03). Serum IGF-1 düzeyi, Grup 1'de 15. günde (P=0.04) motor fonksiyonu ile Grup 2'de 5. gündeki (P=0.011) motor fonksiyonlarıyla korele edildi. Ozon tedavisinin bağ dokusu ve kas dokusunda iyileşme ve rejenerasyon süreci üzerinde olumlu etkileri olabilir ve bu faydalı etkilere IGF-1 aracılı olabilir. IGF-1'in onarım sürecindeki rolünü aydınlatmak ve tedavi stratejilerine ek yeni anlayışlar sağlamak için daha fazla araştırma yapılması gereklidir.

Impacts of Ozone Treatment and Its Relationship with IGF-1 Levels After Injury of Soft Tissue: An Experimental Study in Rats Model

To investigate the effects of ozone treatment on soft tissue injury and to observe whether there is an alteration in serum IGF-1 levels after ozone treatment in an experimental rat model. Twenty-four adult Wistar albino 240-350 g male rats were randomly allocated into two groups. A standardized, experimental soft tissue injury was created on left hind limbs of animals. Group 1 underwent daily ozone treatment intraperitoneally (20 µg/mL), while Group 2 received only nutrition and routine care. All rats were evaluated regarding body weight and sensory and motor function on 5th and 15th days after experimental trauma. Blood samples were drawn from intracardiac in group 1 and group 2 serum levels of IGF-1 level were measured at the day of 15th. During follow-up period after formation of soft tissue injury, three rats in Group 2 had cutaneous infection that responded well to topical tetracycline treatment. Two groups displayed similar results regarding sensory and motor functions on 5th and 15th days. Serum IGF-1 level in Group 1 was significantly higher than that of Group 1 (P=0.03). Serum IGF-1 level was correlated with motor function on day 15 in Group 1 (P=0.04) and with motor function on day 5 in Group 2 (P=0.011). Ozone treatment may have favorable impacts on healing and regeneration process in connective and muscle tissues and these beneficial effects may be mediated by IGF-1. Further research is warranted to elucidate the role of IGF-1 in repair process and to provide additional new insights to the treatment strategies.

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